Potential Impact of Differential Production of the Cry2Ab and Cry1Ac Proteins in Transgenic Cotton in Response to Cold Stress

Addison, Stewart J.; Rogers, D.J.

doi:10.1603/ec09369

Cited by 19 publications

(9 citation statements)

References 83 publications

(106 reference statements)

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“…However, unlike our results showing similar seasonal declines of about threefold in Cry1Ac and Cry2Ab in DP 164 B2RF, previous studies with other cultivars found that the seasonal decline in toxin concentration was generally less for Cry2Ab (35)(36)(37) than Cry1Ac (38)(39)(40)(41)(42). In any case, seasonal declines in the concentration of Bt toxins could be an important factor affecting redundant killing because there is extensive variation among cotton cultivars in patterns of production of Bt toxins (43).…”

Section: Discussioncontrasting

confidence: 98%

Potential shortfall of pyramided transgenic cotton for insect resistance management

Brévault

Heuberger

Zhang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

150

222

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To delay evolution of pest resistance to transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt), the "pyramid" strategy uses plants that produce two or more toxins that kill the same pest. In the United States, this strategy has been adopted widely, with two-toxin Bt cotton replacing one-toxin Bt cotton. Although two-toxin plants are likely to be more durable than one-toxin plants, the extent of this advantage depends on several conditions. One key assumption favoring success of two-toxin plants is that they kill insects selected for resistance to one toxin, which is called "redundant killing." Here we tested this assumption for a major pest, Helicoverpa zea, on transgenic cotton producing Bt toxins Cry1Ac and Cry2Ab. Selection with Cry1Ac increased survival on two-toxin cotton, which contradicts the assumption. The concentration of Cry1Ac and Cry2Ab declined during the growing season, which would tend to exacerbate this problem. Furthermore, analysis of results from 21 selection experiments with eight species of lepidopteran pests indicates that some cross-resistance typically occurs between Cry1A and Cry2A toxins. Incorporation of empirical data into simulation models shows that the observed deviations from ideal conditions could greatly reduce the benefits of the pyramid strategy for pests like H. zea, which have inherently low susceptibility to Bt toxins and have been exposed extensively to one of the toxins in the pyramid before two-toxin plants are adopted. For such pests, the pyramid strategy could be improved by incorporating empirical data on deviations from ideal assumptions about redundant killing and cross-resistance. (Résumé d'auteur

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Section: Discussioncontrasting

confidence: 98%

Potential shortfall of pyramided transgenic cotton for insect resistance management

Brévault

Heuberger

Zhang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

150

222

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“…Similar studies have shown that the expression level of exogenous protein in insect-resistant Bt transgenic corn lines under high temperature, low temperature, drought, and flooding stress conditions was significantly lower than those for plants grown under normal, stress-free growth conditions (Traore et al, 2000; Trtikova et al, 2015). The expression level of exogenous protein in insect-resistant Bt tansgenic cotton lines under salt (Jiang et al, 2006; Luo et al, 2017), flooding (Luo et al, 2008), and low-temperature stress conditions (Addison and Rogers, 2010) was significantly lower than that in plants grown under normal, stress-free conditions. Taken together, the findings of the present and previous studies suggest that external stress conditions might reduce the expression of exogenous Bt protein in transgenic crops.…”

Section: Discussionmentioning

confidence: 99%

Fitness Cost of Transgenic cry1Ab/c Rice Under Saline-Alkaline Soil Condition

Song

Liu

et al. 2018

Front. Plant Sci.

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The environmental release and biosafety of transgenic Bt crops have attracted global attention. China has a large area of saline-alkali land, which is ideal for large-scale production of Bt transgenic rice. Therefore an understanding of the fitness of Bt transgenic rice in saline-alkaline soils and the ability to predict its long-term environmental effects are important for the future sustainable use of these crops. In the present study, we aimed to evaluate the fitness of cry1Ab/c transgenic rice in both farmland and natural ecosystems. Transgenic cry1Ab/c rice Huahui1, for which a national biosafety certificate was obtained, was grown on normal farmland and saline-alkaline soils in a glass greenhouse. The expression pattern of exogenous Cry1Ab/c protein, and vegetative and reproductive fitness of rice were assessed. The expression of the exogenous Cry1Ab/c protein in the transgenic rice grown on saline-alkaline soil was lower than that in the strain grown on farmland soil. Under both the soil conditions, vegetative growth abilities, as evaluated by tiller number and biomass, and reproductive growth abilities, as measured by filled grain number and filled grain weight per plant, showed a significantly higher fitness cost for Huahui1 than that for the parental rice Minghui63 grown under the same soil conditions. In saline-alkaline soil, the fitness cost of Huahui1was moderately higher than that of Minghui63. Therefore, the ecological risk of cry1Ab/c transgenic rice is not expected to be higher than that of parental rice Minghui63 if the former escapes into natural saline-alkaline soil. The results of the present study provide a scientific basis to improve environmental safety assessment of the insect-resistant transgenic rice strain Huahui1 before commercialization.

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“…Plant genotype and parental backgrounds have been recognised as important factors . Other important factors include environmental conditions such as temperature, light, soil properties, waterlogging and drought . In addition, ELISA results commonly vary between studies conducted with different kits, extraction methods and purified standard proteins used.…”

Section: Discussionmentioning

confidence: 99%

Expression of Cry1Ac in transgenicBtsoybean lines and their efficiency in controlling lepidopteran pests

et al. 2013

Pest. Manag. Sci.

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Potential Impact of Differential Production of the Cry2Ab and Cry1Ac Proteins in Transgenic Cotton in Response to Cold Stress

Cited by 19 publications

References 83 publications

Potential shortfall of pyramided transgenic cotton for insect resistance management

Potential shortfall of pyramided transgenic cotton for insect resistance management

Fitness Cost of Transgenic cry1Ab/c Rice Under Saline-Alkaline Soil Condition

Expression of Cry1Ac in transgenicBtsoybean lines and their efficiency in controlling lepidopteran pests

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