Potential immunomodulatory and antioxidant effects of walnut Juglans regia vegetable oil against lead‐mediated hepatic damage and their interaction with lipase activity in rats

Abstract

Environmental pollutants, particularly lead, pose a serious threat to human and animal health that causes disturbances of several functions, including hepatotoxicity. Therefore, the search for a new treatment that could safely and effectively block or reverse liver injuries remains a challenge. This study was carried out to investigate the protective efficacy of Juglans regia vegetable oil (JRVO) against the hepatotoxicity induced by lead. To achieve this aim, adults male rats were treated for 10 days with Pb (0.344 g/kg bw) associated or not with JRVO (0.9 g/kg bw). The rats intoxicated by lead exhibited oxidative stress determined by TBARS, protein carbonyls, liver tumor necrosis factor‐α (TNF‐α), caspase‐3, and antioxidant status: SOD, CAT, GPx, and GSH. Administration of lead increased the levels of plasma hepatic markers (AST, ALT, LDH) and bilirubin, the lipid profiles (total cholesterol, triglycerides, VLDL‐Ch, LDL‐Ch levels, TBARS, NOx, and PCO), the plasmatic lipase activity and the inflammatory markers, while the plasmatic ALP decreased. Coadministration of JRVO restored all the hepatic markers, the lipid profiles and the antioxidants to near‐normal values and lowered the plasmatic lipase activity as well as the elevated thiobarbituric acid reactive substances. Hepatic histological studies confirmed the beneficial role of JRVO through the amelioration of all biochemical parameters. Our results suggest that Juglans regia vegetable oil contains promising substances to counteract the lead intoxication and may be efficient in the prevention of hepatotoxicity complications.

Protective effects of spirulina against hemato-biochemical alterations, nephrotoxicity, and DNA damage upon lead exposition

“…9 In our study, the exposure of adult rats to Pb during 30 days profoundly affected the absolute kidney weights. Similar results have been found by Soussi et al 51 after 15 days of Pb treatment. In this respect, the nutraceutical action of Arthrospira (Spirulina) and some of its metabolites against nephrotoxicity are due to their antiinflammatory and antioxidant properties.…”

Effects of walnut oil on metabolic profile and transcription factors in rats fed high‐carbohydrate‐/‐fat diets

“…Besides oxidative stress reduction efficacy, WO, and components reduce the harmful effects of nitrite-induced toxicity, prevent lead poisoning, and may be effective in preventing hepatotoxicity in the rat liver (Adelakun et al, 2019;Eidi et al, 2013;Soussi, Gargouri, & El Feki, 2018).…”

Beneficial effects of walnut (Juglans regia L.) oil-derived polyunsaturated fatty acid prevents a prooxidant status and hyperlipidemia in pregnant rats with diabetes

“…PUFA was dissolved in 1% CMC solution and administered to rats by oral gavage per day from GD 0 to GD 17. The doses of PUFA were selected according to previous study [18] and our pilot study. The pilot study was performed and results indicated that 900 mg/ kg could be the appropriate dose to exert hypoglycemic effect in the present study.…”