Potential for fast chlorophyll<i>a</i>fluorescence measurement in bryophyte ecophysiology

Liepiņa, Ligita; Ievinsh, Gederts

doi:10.3176/eco.2013.2.05

Cited by 6 publications

(7 citation statements)

References 40 publications

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“…In the context of biomonitoring studies it should be remembered that mosses are not only chemical adsorbent but also living organisms [32]. That is why biomonitoring studies with the use of mosses should focus on monitoring of their life parameters, such as, for example, chlorophyll content or fluorescence [33].…”

Section: Introductionmentioning

confidence: 99%

Influence of Heavy Metal Concentration on Chlorophyll Content in Pleurozium schreberi Mosses

Świsłowski

Rajfur

Wacławek

2020

Ecological Chemistry and Engineering S

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The aim of biomonitoring is assessment of environment condition. Biomonitoring studies with the use of mosses focus mainly on analytes accumulation and determining elements’ concentrations in the study area. It is often forgotten that a bioindicator should be alive during biomonitoring studies (which can be determined by, e.g., analysis of chlorophyll content). The objective of the carried out research was an assessment of the influence of selected heavy metals concentration: Ni, Cu, Zn, Cd and Pb accumulated by Pleurozium schreberi mosses during 3-month exposition within active biomonitoring, on their vitality, assessed by an analysis of a and b chlorophyll concentrations. The studies were also carried out in laboratory conditions, where the content of the dyes was determined with the aid of a UV-Vis spectrophotometer, in mosses reacting with solutions of various concentrations of the analysed metals. The content of elements in mosses after exposition and in solutions prior and after sorption were determined with the use of atomic absorption spectrometry (AAS) in a flame atomiser. After the carried out studies it was determined that mosses, during 12-week long exposition, accumulated heavy metals, which did not clearly influence the changes in chlorophyll content. The carried out studies prove that heavy metals are not the only and determining factor, which influences chlorophyll content in mosses as well as the bioindicator’s vitality in the conditions of environmental stress.

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Section: Introductionmentioning

confidence: 99%

Influence of Heavy Metal Concentration on Chlorophyll Content in Pleurozium schreberi Mosses

Świsłowski

Rajfur

Wacławek

2020

Ecological Chemistry and Engineering S

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“…One might argue that the distribution of species within the defined continuum of tissue water content, pigment concentration and chlorophyll a fluorescence traits may have a stochastic character. Variability of characteristics within particular species was indeed relatively high, as it was already noted in a previous study (Liepiòa and Ievinsh, 2013). It was attempted to eliminate any seasonal dependence of the measured traits by sampling the same bryophyte species four times during summer.…”

Section: Discussionmentioning

confidence: 81%

“…For bryophytes, this type of analysis has been used mostly in dehydration/rehydration experiments as an indication of the state of photosynthetic apparatus (Proctor, 2003;Beckett et al, 2005;Cruz de Carvalho et al, 2011). Recently, it has been shown that the fast chlorophyll a fluorescence method can provide quantitative estimates of the photochemical activity of bryophytes with potential use in ecophysiological studies (Liepiòa and Ievinsh, 2013). In addition, photosynthetic pigment concentration has been used as an indicator of photosynthetic activity in desiccation experiments (Martin and Warner, 1984) as well as in ecophysiology (Lopez and Carballeira, 1989), e.g., for characterisation of the relationship with light availability (Marschall and Proctor, 2004).…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Functional Traits, Functional Types, and Habitat in Boreonemoral Bryophytes

Ievinsh

Gaile

Elferts

et al. 2020

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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The aim of the present study was to determine a relationship between physiological traits and functional types of bryophytes from five boreonemoral habitats with a particular emphasis on discriminative ability of these traits. Sampling of 25 species was performed four times during one season. Water content, chlorophyll a fluorescence and photosynthetic pigment concentration were measured in field and water-equilibrated samples. Principal component analysis indicated the existence of an inverse relationship between concentration of pigments and water content. Linear discriminant analysis showed that relatively high mean predicted posterior probabilities of correct classification of functional types by physiological traits were found for water conducting system, followed by substrate and habitat, but it was highly variable and type-specific for life form and relatively less variable for life strategy. Field water content had the highest average discriminative importance among physiological traits, followed by chlorophyll fluorescence indices field Fv/Fm, equilibrated PI, equilibrated RC/ABS, and field RC/ABS. Photosynthetic pigment concentrations had relatively less average importance for classification of functional types.

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“…Most previous studies of moss chlorophyll fluorescence (Rau et al, 2007; Proctor and Smirnoff, 2011; Liepiņa and Ievinsh, 2013; Kangas et al, 2014; Jägerbrand and Kudo, 2016) focused on the fluorescence value changes. Our former studies (Chen et al, 2015a, 2018a) and the data proposed in this perspective suggest that color patterns of the fluorescence images could reflect metal species groups and concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…Moss rhizoid is too short to monitor deep soil pollution and therefore it is only used to monitor heavy metals in aquatic environment accurately. Based on the previous researches using chlorophyll fluorescence to detect abiotic stresses in the aquatic bioindicator mosses (Rau et al, 2007; Proctor and Smirnoff, 2011; Liepiņa and Ievinsh, 2013; Kangas et al, 2014; Jägerbrand and Kudo, 2016), we provided new ideas to monitor water heavy metals rapidly and non-invasively in a large-scale by moss visible parameters of Fv/Fm and Φ PSII (Chen et al, 2015a). In other words, metal contaminations could be roughly estimated visually using the chlorophyll fluorescence images.…”

Section: Introductionmentioning

confidence: 99%

Perspective of Monitoring Heavy Metals by Moss Visible Chlorophyll Fluorescence Parameters

Chen

Zhang

et al. 2019

Front. Plant Sci.

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Chlorophyll fluorescence measurements have been mainly applied to investigate the functioning of the photosynthetic apparatus in the diagnosis of environmental stress. Moss is sensitive to several abiotic stresses and is considered an environmental indicator. Therefore, moss chlorophyll fluorescence can be as a visual parameter applicable for monitoring heavy metal contaminants in water. Different from previous studies with value changes of chlorophyll fluorescence in mosses, we suggest that phenotypes with anthocyanin accumulation pattern and chlorosis pattern and colors of chlorophyll fluorescence images of the maximum efficiency of PSII photochemistry (Fv/Fm) and the quantum yield of PSII electron transport (ΦPSII) could reflect metal species groups and concentrations roughly. And we further indicated that Cr(III) and Cr(VI) could be monitored distinguishably according to the non-photochemical quenching (NPQ) fluorescence of sporadic purple and sporadic lavender images, respectively. It is interesting that the fluorescence color patterns were nearly the same for all treatment concentrations. This perspective provides additional data of chlorophyll fluorescence changes in moss under cold, heat, salinity, high light or osmotic stress. Only heat stress and high light have significant effects on the fluorescence parameters of Fv/Fm and ΦPSII. In contrast, mosses are less sensitive to short-term cold, salinity, and osmotic stress. While NPQ decreases rapidly under the osmotic stress. Nevertheless, heat stress, high light or osmotic stress does not usually co-occur in the place where the moss grows. Estimation through moss chlorophyll fluorescence color patterns is still a rapid and non-invasive method to monitor heavy metal pollutions in water.

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Potential for fast chlorophyllafluorescence measurement in bryophyte ecophysiology

Cited by 6 publications

References 40 publications

Influence of Heavy Metal Concentration on Chlorophyll Content in Pleurozium schreberi Mosses

Influence of Heavy Metal Concentration on Chlorophyll Content in Pleurozium schreberi Mosses

Relationship Between Functional Traits, Functional Types, and Habitat in Boreonemoral Bryophytes

Perspective of Monitoring Heavy Metals by Moss Visible Chlorophyll Fluorescence Parameters

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