Potential for biodiesel production and carbon capturing from Synechococcus Elongatus: An isolation and evaluation study

Mashayekhi, M.; Sarrafzadeh, Mohammad‐Hossein; Tavakoli, Omid; Soltani, Nepton; Faramarzi, Mohammad Ali

doi:10.1016/j.bcab.2017.01.005

Cited by 22 publications

(13 citation statements)

References 39 publications

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“…Dry cell weight and biomass productivity of the Synechococcus elongatus during 16 days of cultivation [reprinted with permission from Elsevier] …”

Section: Comparison Between Varieties Of Cyanobacteriamentioning

confidence: 99%

“…The biomass and lipid productivity of these cyanobacteria increased when the wastewater containing chromium Cr(VI) was used instead of BG‐11 medium and the best yield was observed in 15 mg/L Cr(VI) (Figure ). Mashayekhi et al isolated a freshwater cyanobacterium, Synechococcus elongatus , from the Ramsar hot springs, Iran. The culture of the isolate in BG‐11 medium under the pH of 9, 72 mL/min flow rate, and 2% CO 2 produced the highest growth rate.…”

Section: Comparison Between Varieties Of Cyanobacteriamentioning

confidence: 99%

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Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Farrokh

Sheikhpour

Kasaeian³

et al. 2019

Biotechnology Progress

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Cyanobacteria are photosynthetic microorganisms which can be found in various environmental habitats. These photosynthetic bacteria are considered as promising feedstock for the production of the third‐ and the fourth‐generation biofuels. The main subject of this review is highlighting the significant aspects of the biofuel production from cyanobacteria. The most recent investigations about the extraction or separation of the bio‐oil from cyanobacteria are also adduced in the present review. Moreover, the genetic engineering of cyanobacteria for improving biofuel production and the impact of bioinformatics studies on the designing better‐engineered strains are mentioned. The large‐scale biofuel production is challenging, so the economic considerations to provide inexpensive biofuels are also cited. It seems that the future of biofuels is strongly dependent to the following items; understanding the metabolic pathways of the cyanobacterial species, progression in the construction of the engineered cyanobacteria, and inexpensive large‐scale cultivation of them.

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“…Dry cell weight and biomass productivity of the Synechococcus elongatus during 16 days of cultivation [reprinted with permission from Elsevier] …”

Section: Comparison Between Varieties Of Cyanobacteriamentioning

confidence: 99%

Section: Comparison Between Varieties Of Cyanobacteriamentioning

confidence: 99%

Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Farrokh

Sheikhpour

Kasaeian³

et al. 2019

Biotechnology Progress

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“…antibiotics and sterilization by ltration) using raceway ponds for cultivation suggest that the PtxD/Phi system would decrease operating costs. Using Phi as the P source and contamination control, operating costs would be 15.47 and 37 % lower (Supplementary Table 5 and 6, Additional File 2) than using antibiotic [9,50] and sterilization by ltration, respectively [51,52,53]. The cost of cultivation in open ponds is substantially lower than that of using other types of bioreactors, suggesting the PtxD/Phi system has great promise to contributing to overcome the contamination issue and open the possibility of implementation of this type of bioreactor to cultivate many other microalgae/cyanobacteria species to produce biofuels and other valuable products.…”

Section: Discussionmentioning

confidence: 99%

Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds

González-Morales¹,

Pacheco-Gutiérrez²,

Ramírez-Rodríguez³

et al. 2020

Preprint

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Background: The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by public and private sectors to develop this field. However, it has been a challenge to develop a viable and cost-effective platform for cultivation of cyanobacteria and microalgae under outdoor conditions. Dealing with contamination caused by bacteria, weedy algae/cyanobacteria and other organisms is a major constraint to establish effective cultivation processes.Results: Here we describe the implementation in the cyanobacterium Synechococcus elongatus PCC 7942 of a phosphorus selective nutrition system to control biological contamination during cultivation. The system is based on metabolic engineering of S. elongatus to metabolize phosphite, a phosphorus source not normally metabolized by most organisms, by expressing a bacterial phosphite oxidoreductase (PtxD). Engineered S. elongatus strains expressing PtxD grow at a similar rate on media supplemented with phosphite as the non-transformed control supplemented with phosphate. We show that when grown in media containing phosphite as the sole phosphorus source in glass flasks, the engineered strain was able to grow and outcompete biological contaminants even when the system was intentionally inoculated with natural competitors isolated from an irrigation canal. The PtxD/phosphite system was successfully used for outdoor cultivation of engineered S. elongatus in 100 L cylindrical reactors and 1,000 L raceway ponds, under non-axenic conditions and without the need of sterilizing containers and media. Finally, we also show that the PtxD/phosphite system can be used as selectable marker for S. elongatus PCC 7942 transgenic strains selection, eliminating the need of antibiotic resistance genes. Conclusions: Our results suggest that the PtxD/phosphite system is a stable and sufficiently robust strategy to control biological contaminants without the need of sterilization or other complex aseptic procedures. Our data shows that the PtxD/phosphite system can be used as selectable marker and allows production of the cyanobacterium S. elongatus PCC 7942 in non-axenic outdoor reactors at lower cost, which in principle should be applicable to other cyanobacteria and microalgae engineered to metabolize phosphite.

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“…Synechococcus elangatus cyanobacterium was taken from Ramsar hot spring (Mazandaran, Iran). It was provided after isolation, preparing axenic culture and characterizing the strain . KOH, HCl (37%), Ethanol (99.8%), Nafion (5 wt.% in lower aliphatic alcohols and water), and Pt/C 20 wt.% on Vulcan XC72 were purchased from Sigma‐Aldrich, Germany.…”

Section: Methodsmentioning

confidence: 99%

“…Cyanobacteria, algae and plants use the CO 2 and light for photosynthesis and release the atmospheric oxygen and produce most of the latent chemical energy which is necessary for the earth life. Due to fast growth rates, cyanobacteria have high potential for bio‐chemicals production and fossil fuel substitution , .…”

Section: Introductionmentioning

confidence: 99%

One Step Synthesis of Tertiary Co‐doped Graphene Electrocatalyst Using Microalgae Synechococcus elangatus for Applying in Microbial Fuel Cell

Hassani¹,

Ziaedini

Samiee³

et al. 2019

Fuel Cells

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In this work, heteroatom‐doped porous graphene was synthesized by pyrolysis method using microalgae Synechococcus elangatus as a biomass resource. The prepared samples were characterized by X‐ray diffraction (XRD), N2 sorption‐desorption, field emission scanning electron microscopy (FESEM) and X‐ray photoelectron spectroscopy (XPS). The electrochemical behavior of the synthesized samples was investigated for oxygen reduction reaction (ORR) and evaluated using microbial fuel cell (MFC). The results revealed that the catalytic activity of the prepared sample including N, S and P atoms on porous graphene (PG) was close to the Pt/C 20 wt.%. According to the linear sweep voltammetry (LSV) measurements, the onset potential of optimal sample (0.97 V versus RHE) was close to the Pt/C 20 wt.% (0.99 V versus RHE). Furthermore, the stability test demonstrated much better tolerance to the methanol crossover effects for the optimal sample in comparison to the Pt/C 20 wt.%. Moreover, the microbial fuel cell (MFC) test showed that the cell potential of the optimal sample is close to Pt/C 2 wt.%, and represented a high peak power density of 31.5 mW m−2, which is comparable to the Pt/C 20wt.% (38.6 mW m−2) cathodes, because of synergistic effect of N, S and P co‐doped carbon structure, which leads to improvement in catalytic activity.

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Potential for biodiesel production and carbon capturing from Synechococcus Elongatus: An isolation and evaluation study

Cited by 22 publications

References 39 publications

Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Cyanobacteria as an eco‐friendly resource for biofuel production: A critical review

Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds

One Step Synthesis of Tertiary Co‐doped Graphene Electrocatalyst Using Microalgae Synechococcus elangatus for Applying in Microbial Fuel Cell

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