Potential enzyme toxicity of perfluorooctanoic acid

Abstract

Using equilibrium dialysis, isothermal titration calorimetry (ITC) and circular dichroism (CD), the interactions of perfluorooctanoic acid (PFOA) and lysozyme were investigated under normal human physiological conditions, i.e., at pH 4.40, 6.00 and 7.40 at 37 degrees C in 0.15 M electrolyte. A simple and rapid spectrophotometric method was developed for determining PFOA concentrations. Interactions between PFOA and lysozyme were found to result from non-specific non-covalent bonds-F/N and F/O affinity, ion-pair attraction, hydrogen bond, hydrophobic interaction and van der Waals force-and were affected by chemical adsorption to monolayers. The results indicated that binding of PFOA altered the secondary structure and activity of lysozyme. This work provides a useful experimental strategy for research into the enzyme toxicity of organic chemicals, e.g., food additives and organic contaminants, and it may help to elucidate the molecular toxicology of human health risks.

Interaction of perfluorooctanoic acid with human serum albumin

“…The anionic color ligand eriochrome cyanine R (ECR) and cationic surfactants cetylpyridinium chloride (CPC) were employed to determine PFOA. The detailed analytical procedure was described by [ 32 ].…”

Probing the toxic mechanism of Ag+ with lysozyme

“…For example, lysozyme plays the role of sterilization by destroying Gram-positive bacterial cell wall to protect the cells from death [10][11][12]. In addition to the antibacterial, it also can be used as antioxidant, antiseptic and antiviral [13][14][15][16]. Lysozyme is widely found in eukaryotes and prokaryotes [17].…”

Transmembrane transports of acrylamide and bisphenol A and effects on development of zebrafish (Danio rerio)

“…During the transportation process, the chemical must cross the cell membrane, which consists of a phospholipid bilayer and membrane proteins linked to oligosaccharides, and then bind to biomolecules in the cytoplasm before being transported to the nucleus. The mechanisms of toxicity of exogenous chemicals have been elucidated by many studies, which have often focused on characterizing interactions with target molecules [1][2][3][4][5], errors in protein expression [6], alteration of gene sequences [7] and dose-effect relationships [8]. In contrast, transmembrane and intracellular transport of harmful chemicals has received little attention [9].…”