Potential Elimination of Human Gut Resistome by Exploiting the Benefits of Functional Foods

Tsigalou, Christina; Konstantinidis, Theocharis; Stavropoulou, Elisavet; Bezirtzoglou, Eugenia; Tsakris, Athanasios

doi:10.3389/fmicb.2020.00050

Cited by 29 publications

(25 citation statements)

References 82 publications

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“…Although the gut microbiome may be considered as the basis of the host’s wellbeing, at the same time it creates potential threats due to the presence of ARgenes (antibiotic resistance genes). It could be a “reservoir” of Multi Drug Resistant Bacteria (MDR) or Pan Drug Resistant Bacteria (PDR) and their antibiotics resistance genes [ 127 ]. Taken together, the ARgenes and their ancestors of pathogenic and non-pathogenic gut bacteria comprise the “resistome”, as proposed by Gerard D. Wright in 2006 and 2007 [ 128 , 129 ].…”

Section: The Reservoir Of Antibiotics In Animal Feed and The Emergmentioning

confidence: 99%

“…However, it seems that the majority of these determinants are considered innate and are not shared with opportunistic pathogens [ 130 ]. Furthermore, the “mobilome”, which consists of MGEs, serves as a path for transferring ARgenes among intestinal bacteria [ 127 ]. Metagenomic research revealed that, after extended antibiotic treatment, especially with aminoglycosides, an augmentation in the relative abundance of ARgenes emerged [ 131 , 132 ].…”

Section: The Reservoir Of Antibiotics In Animal Feed and The Emergmentioning

confidence: 99%

“…Clostridium difficile is a well-known factor causing nosocomial diarrhea because of prolonged broad-spectrum antibiotic treatment, and it is worth stating that probiotics (beneficial microbes for the gut) together with antibiotics might prevent clinical infections [ 133 ]. Probiotic bacteria as well as dietary interventions could be very promising, either preventing undesired shifts in the gut microbiome due to antibiotics or restoring the harmed balance after detrimental antibiotic use [ 127 , 134 , 135 ].…”

Section: The Reservoir Of Antibiotics In Animal Feed and The Emergmentioning

confidence: 99%

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Effects of Antibiotics upon the Gut Microbiome: A Review of the Literature

et al. 2020

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The human gastrointestinal tract carries a large number of microorganisms associated with complex metabolic processes and interactions. Although antibiotic treatment is crucial for combating infections, its negative effects on the intestinal microbiota and host immunity have been shown to be of the utmost importance. Multiple studies have recognized the adverse consequences of antibiotic use upon the gut microbiome in adults and neonates, causing dysbiosis of the microbiota. Repeated antibiotic treatments in clinical care or low-dosage intake from food could be contributing factors in this issue. Researchers in both human and animal studies have strived to explain this multifaceted relationship. The present review intends to elucidate the axis of the gastrointestinal microbiota and antibiotics resistance and to highlight the main aspects of the issue.

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Section: The Reservoir Of Antibiotics In Animal Feed and The Emergmentioning

confidence: 99%

Section: The Reservoir Of Antibiotics In Animal Feed and The Emergmentioning

confidence: 99%

Section: The Reservoir Of Antibiotics In Animal Feed and The Emergmentioning

confidence: 99%

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Effects of Antibiotics upon the Gut Microbiome: A Review of the Literature

et al. 2020

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“…The development of new antibiotics for active infection with MDR bacteria is very slow, highlighting the need for alternative strategies to prevent the spread of antibiotic-resistant bacteria. Among these strategies, the effectiveness of reducing colonization of the intestinal microbiota by MDR bacteria using probiotics is being supported by in vitro and clinical observations ( 7 , 8 ). The use of probiotic strains such as Lactiplantibacillus plantarum or Limosilactobacillus fermentum (formerly named Lactobacillus plantarum or Lactobacillus fermentum ) was associated with a reduction in colonization with naturally resistant pathogens, such as Acinetobacter baumannii, Pseudomonas aeruginosa , and Candida albicans ( 9 , 10 ).…”

Section: Introductionmentioning

confidence: 99%

Do Probiotics During In-Hospital Antibiotic Treatment Prevent Colonization of Gut Microbiota With Multi-Drug-Resistant Bacteria? A Randomized Placebo-Controlled Trial Comparing Saccharomyces to a Mixture of Lactobacillus, Bifidobacterium, and Saccharomyces

Wieërs

Verbelen

Driessche³

et al. 2021

Front. Public Health

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Objective: Most infections with Enterobacteriaceae producing AmpC β-lactamase (AmpC)-, extended-spectrum β-lactamase (ESBL)-, and carbapenemase-producing bacteria, vancomycin-resistant Enterococcus as well as naturally resistant non-fermenting bacteria such as Pseudomonas aeruginosa, are related to a prior colonization of the gut microbiota. The objective of this study was to determine whether treatment with probiotics during an antibiotic treatment could prevent the colonization of the gut microbiota with multi-drug resistant bacteria.Method: In total, 120 patients treated for 10 days with amoxicillin-clavulanate antibiotics were included in a randomized, placebo-controlled, double-blinded trial, comparing the effects of a 30 days treatment with placebo Saccharomyces boulardii CNCM I-745® and a probiotic mixture containing Saccharomyces boulardii, Lactobacillus acidophilus NCFM, Lactobacillus paracasei Lpc-37, Bifidobacterium lactis Bl-04, and Bifidobacterium lactis Bi-07 (Bactiol duo®). Study treatment was initiated within 48 h of the antibiotic being initiated. Most of the patients included were elderly with a mean age of 78 years old with multiple comorbidities. Stools were collected at the time of inclusion in the trial, at the end of the antibiotic treatment, and the end of the study treatment. These were cultured on selective antibiotic media.Results: Treatment with the probiotic mixture led to a significant decline in colonization with Pseudomonas after antibiotic treatment from 25 to 8.3% (p = 0.041). Colonization with AmpC-producing enterobacteria was transiently increased after the antibiotic treatment (p = 0.027) and declined after the probiotic intervention (p= 0.041). No significant changes were observed in the placebo and Saccharomyces groups. Up to 2 years after the trial, no infection with ESBL-producing bacteria was observed in the probiotic mixture group.Conclusion: The association of Saccharomyces boulardii with specific strains of Lactobacillus and Bifidobacterium influences antibiotic treatment by counteracting the colonization of the colon microbiota with antibiotic-resistant pathogens.

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“…The increased abundance of Bacteroidetes in the fecal microbiota of healthy controls is supported by the fact that Bacteroidetes are the major representatives of the normal gut [ 54 ]. Bacteroidetes were identified as the most abundant phylum in both healthy and diseased subjects; however, their relative abundances varied greatly ( Figure 1 , Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing

Afridi

Chang

2021

Microorganisms

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In this paper, we aimed to characterize the fecal microbiome and its resistomes of healthy and diseased subjects infected with multidrug-resistant Escherichia coli using next-generation sequencing (NGS). After initial screening, 26 stools samples belonging to healthy (n = 13) and diseased subjects (n = 13) were selected and subjected to NGS. A total of 23 and 42 antibiotic-resistant genes (ARGs) conferring resistance to 6 and 9 classes of antibiotics were identified in the resistomes of healthy and diseased subjects, respectively. Bacteroidetes were found to be the major phylum in both healthy and diseased subjects; however, Proteobacteria was predominantly present in the diseased subjects only. Microbial dysbiosis and predominance of various ARGs in the resistome of diseased subjects reflect the excessive usage of antibiotics in Pakistan and warrants immediate attention to regulate the use of various antimicrobials.

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Potential Elimination of Human Gut Resistome by Exploiting the Benefits of Functional Foods

Cited by 29 publications

References 82 publications

Effects of Antibiotics upon the Gut Microbiome: A Review of the Literature

Effects of Antibiotics upon the Gut Microbiome: A Review of the Literature

Do Probiotics During In-Hospital Antibiotic Treatment Prevent Colonization of Gut Microbiota With Multi-Drug-Resistant Bacteria? A Randomized Placebo-Controlled Trial Comparing Saccharomyces to a Mixture of Lactobacillus, Bifidobacterium, and Saccharomyces

Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing

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