Potential effects of alpha-pinene, a monoterpene commonly found in essential oils against Toxoplasma gondii infection; an in vitro and in vivo study

Kharazmkia, Ali; Al-Abodi, Hiba Riyadh; Yadegari, Javad Ghasemian; Vahidi, Ashkan; Mahmoudvand, Hossein

doi:10.1007/s12639-022-01514-1

Cited by 7 publications

(10 citation statements)

References 40 publications

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“…effects of the flavonoids and phenolic compounds (Lehane & Saliba, 2008;Ram‫ם‬rez-Mac‫ם‬as et al, 2012;Naithani et al, 2008;Lucchini et al, 1990;Mead & McNair, 2006). In addition, previous investigations have indicated that the antimicrobial mechanisms of the flavonoids, and phenolic compounds are associated to their impact on the nucleic acid synthesis suppression, cytoplasmic membrane dysfunction, energy metabolism dysfunction, inhibition of bacterial virulence factors, exhibition a synergistic effect with existing synthetic drugs, etc (Chusnie & Lamb, 2005;Bouarab Chibane et al, 2019;Kharazmkia et al, 2022;Mahmoudvand et al, 2022;Yadegari et al, 2022). On the other hand, investigations have demonstrated that the flavonoids and phenolic compounds through the strengthens the immune system mostly cellular immune system through the mTOR pathway signaling activity, stimulating immune cells (e.g., macrophages, and natural killer cells), regulation of cytokine excretion, phagocytosis, triggering of macrophages, and production of immunoglobulins are able to control and eliminate the microbial infections (Mendes et al, 2019;Chiang et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Stachys lavandulifolia Vahl. exhibits promising in vitro and in vivo antileishmanial activity against Leishmania major infection

A.D.¹

2022

Trop Biomed

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This study aimed to consider the in vitro and in vivo effects of the Stachys lavandulifolia methanolic extract (SLME) (2.5, 5, 10, 25, 50, 100 µg/mL) against Leishmania major infection. The in vitro antileishmanial effects of SLME was studies on promastigote and amastigote forms of L. major. The effect of SLME on the nitric oxide (NO) and apoptosis, secretion of Th1/2 cytokines, and infectivity rate in macrophages cells were also studies. The cytotoxicity of SLME on human (THP-1) and murine (J774-A1 cell) macrophage cells was investigated through the measuring the 50% cytotoxic concentrations (CC 50 ). Moreover, the in vivo effects of SLME for healing the cutaneous leishmaniasis (CL) lesions in infected BALB/c mice studied by assessing the lesions size and the parasite load during four weeks of treatment. The calculated 50% inhibitory concentration (IC 50 ) values for SLME and meglumine antimoniate (MA) against the promastigote stage were 23.4 and 71.1 µg/mL, respectively. For amastigote stage, the IC 50 values for SLME and MA were 39.3 µg/mL and 44.3 µg/mL, respectively. Followed by 28 days' topically therapy with SLME at doses of 50 and 100 mg/kg/day, the CL lesions size as well as parasite load were significantly (p<0.001) reduced; such that the recovery percentage of the infected mice was 80% and 97% after treatment with SLME at the dose of 50 and 100 mg/kg, respectively. SLME also markedly induced the NO production and apoptosis; whereas decreased infection rate in macrophage cells. After incubation of infected macrophages with SLME, the level interferon gamma was meaningfully (p<0.001) elevated as a dose-dependent response; in contrast, release of interleukin 10 (IL-10) and IL-4 markedly (p<0.001) decreased. The CC 50 value for SLME against THP-1 and J774-A1 cell was 996.4 µg/mL and 741.3 µg/mL, respectively. The calculated selectivity index of >10 for SLME and MA confirmed their specificity to amastigotes and the low toxicity for macrophages. Our results showed the potent effects of SLME in eliminating and controlling Leishmania parasites in both in vitro and in vivo assays. Based on the current experimental study, SLME can be suggested as an alternative medicine for the isolation and production of a new agent for treating CL caused by L. major. Although, we found some cellular mechanisms of SLME against Leishmania parasites, but, additional surveys are necessary to specify the accurate mechanisms of action, toxicity, and its efficacy mainly in human subjects.

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Section: Resultsmentioning

confidence: 99%

Stachys lavandulifolia Vahl. exhibits promising in vitro and in vivo antileishmanial activity against Leishmania major infection

A.D.¹

2022

Trop Biomed

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“…( Porres-Martínez et al, 2016 ). The treatment of mice induced by acute toxoplasmosis with a-pinene for 14 consecutive days significantly reduced the serum level of MDA as a principal marker of lipid peroxidation and one of the oxidative stress consequences ( Kharazmkia et al, 2022 ). Similarly, studies have shown that α-pinene, acting as an antioxidant, reduces lipid peroxidation and enhances CAT and SOD activities in the cortical and hippocampal regions in a rat model of focal ischemic stroke ( Khoshnazar et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of alpha-pinene against behavioral deficits in ketamine-induced mice model of schizophrenia: Focusing on oxidative stress status

Hajizadeh Moghaddam,

Malekzadeh Estalkhi,

Khanjani Jelodar

et al. 2024

IBRO Neuroscience Reports

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“…Carvacrol had effects on bacterial membrane ion gradients, even without causing significant ATP leakage [ 110 ]. Alpha-pinene induced apoptosis by increasing the caspase-3 activity [ 118 ], but had significant synergistic interactions as well, which might be promising to various therapeutic fields [ 119 ].…”

Section: Discussionmentioning

confidence: 99%

Development of a novel, entirely herbal-based mouthwash effective against common oral bacteria and SARS-CoV-2

Bencze

Temesfői

Das

et al. 2023

BMC Complement Med Ther

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Background Parallel to the growth of the oral healthcare market, there is a constantly increasing demand for natural products as well. Many customers prefer products that contain fewer toxic agents, therefore providing an environmentally friendly solution with the benefit of smaller risk to the user. Medieval and early modern medicinal knowledge might be useful when looking for natural, herbal-based components to develop modern products. Along with these considerations we created, tested, and compared an entirely natural mouthwash, named Herba Dei. Methods The manufacturing procedure was standardized, and the created tincture was evaluated by GC/MS analysis for active compounds, experimentally tested in cell-based cytotoxicity, salivary protein integrity, cell-free antioxidant activity, anti-bacterial and anti-viral assays, and compared with three market-leading mouthwashes. Results Our tincture did not show significant damage in the cytotoxicity assays to keratinocyte and Vero E6 cells and did not disrupt the low molecular weight salivary proteins. Its radical scavenging capacity surpassed that of two tested, partly natural, and synthetic mouthwashes, while its antibacterial activity was comparable to the tested products, or higher in the bacterial aerobic respiratory assay. The active compounds responsible for the effects include naturally occurring phenylpropanoids, terpenes, and terpenoids. Our mouthwash proved to be effective in vitro in lowering the copy number of SARS-CoV-2 in circumstances mimicking the salivary environment. Conclusions The developed product might be a useful tool to impede the transmission and spread of SARS-CoV-2 in interpersonal contact and aerosol-generating conditions. Our mouthwash can help reduce the oral bacterial flora and has an antioxidant activity that facilitates wound healing and prevents adverse effects of smoke in the oral cavity.

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Potential effects of alpha-pinene, a monoterpene commonly found in essential oils against Toxoplasma gondii infection; an in vitro and in vivo study

Cited by 7 publications

References 40 publications

Stachys lavandulifolia Vahl. exhibits promising in vitro and in vivo antileishmanial activity against Leishmania major infection

Stachys lavandulifolia Vahl. exhibits promising in vitro and in vivo antileishmanial activity against Leishmania major infection

Neuroprotective effects of alpha-pinene against behavioral deficits in ketamine-induced mice model of schizophrenia: Focusing on oxidative stress status

Development of a novel, entirely herbal-based mouthwash effective against common oral bacteria and SARS-CoV-2

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