Potential effect of twoBacillusprobiotic strains on performance and fecal microbiota of breeding sows and their piglets

Abstract

The effect of long-term administration of two Bacillus strains was tested on 98 breeding sows and their litters allotted into three treatments: a control group (CON); supplemented with 5x10 8 cfu/kg B. subtilis – 541 (BSU); or with 5x10 8 cfu/kg B. amyloliquefaciens – 516 (BAM). Reproductive and performance variables were recorded over three cycles with 56 dams remaining through the third lactation. Blood and fecal samples were taken longitudinally from 12 sows per treatment on days 8 and 21 of the third lactation and milk samples were taken on day 21. Feces from one piglet per litter was sampled on days 21 and 33 and jejunal gene expression was assessed in two piglets on day 21. Changes in fecal microbiota were assessed by 16S rRNA gene sequencing (Illumina MiSeq) and gene expression by Open-Array technology. Metabolomic responses were analyzed in milk by NMR and Ig-G and Ig-A specific antibodies were determined by ELISA. No significant differences were observed on feed intake, body weight, or fat mobilization of the sows. However, a significant increase in the total number of piglets born was observed in supplemented sows. Whereas the increase was seen from the first cycle with BAM, improvements were not seen with BSU until the third cycle. BAM also increased the number of born-alive and weaned piglets. NMR analysis showed an impact of BAM on milk composition. No differences were found in milk or blood immunoglobulins. A different structure of the fecal microbiota was found in supplemented sows, with changes across phylum, family, and genus. These changes were greater at day 8, suggesting a relevant role of probiotics establishing a new intestinal balance after labor. Shifts in the microbiota were also seen in the piglets, with a clearer impact post-weaning than in suckling. In this regard, correlations between microbial groups of sows and piglets showed a higher link with weaned (d33) than with suckling pigs (d21) reinforcing the idea of an early maternal carry-over. No changes due to treatment in jejunal gene expression were detected, however, piglet size had a clear impact on different genes. In summary, the addition of both probiotics, and particularly Bacillus amyloliquefaciens, demonstrated potential benefits on the prolificacy of sows. Daily feeding of Bacillus amyloliquefaciens resulted in an increase in the number of weaned piglets. The high correlations between the compositions of the microbiota of sows and their piglets is evidence of maternal imprinting, with effects lasting beyond weaning.

Effects of Bacillus subtilis supplementation on reproductive parameters during late gestation in multiparous sowsgestation in multiparous sows

“…In the present study, the insignificant effect of probiotic on the number of total born and the number of born alive is corroborated by those found in several previous studies [ 12 , 29 - 32 ]. In contrast, some authors found that the number of total born and the number of born alive in the probiotic groups were higher than those in the untreated groups [ 17 – 20 , 28 ]. It is clear that probiotic supplementation during late gestation did not have any effects on the number of total born since this parameter was already fixed before probiotic use.…”

“…Several probiotics have been supplemented during different stages of gestation to promote the reproductive performance and health of the sows [12][13][14][15][16], with controversial results. Some probiotics have been shown to increase the number of piglets born alive [17][18][19][20], litter birth weight [19,[21][22][23], and birth weight of piglets [21,24]. On the other hand, some probiotics reduced the birth weight of piglets [13,18,25].…”

Characterization of the Effects of a Novel Probiotic on Salmonella Colonization of a Piglet-Derived Intestinal Microbiota Using Improved Bioreactor

“…After 24 h of treatment, the continuous treatment samples differentiated more from the control when compared to the daily dose treatment, and this remained true over time ( Figure 2 ). Some studies showed no significant change in beta diversity in the gut microbiota when administrating a Bacillus -based probiotic to swine [ 41 , 43 ], but others did [ 40 , 44 ]. This might be due to the length that the treatments were administered, the mode of administration, or a difference in the product tested.…”

Gut Health and Influencing Factors in Pigs

“…Furthermore, maternal consumption of a fermented diet protected neonates from colonic inflammation by facilitating the maturation of the gut microbiota and proliferation of gut lactobacillus [ 189 ]. The strong correlations between the microbiota compositions of sows and their offspring are proof of maternal imprinting, which has benefits that persist after weaning [ 190 ].…”