Potential Diazo Reagents for Colorimetric Determination

Urbányi, Tibor; Mollica, Joseph A.

doi:10.1002/jps.2600570746

Cited by 2 publications

(2 citation statements)

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“…Although no underlying kinetic study was undertaken and single points were used as read-outs, the authors concluded that electron-poor systems are the better analytical probes for rapid screenings. 530 Diazotized sulfanilic acid 206 (Pauly's or Ehrlich's diazo reagent) has been used as early as 1883 by Ehrlich for the detection of bilirubin in urine samples 531 and has found many applications in the detection of phenolic derivatives since then. 532−535 Its electron-poor character, however, makes it unstable; fresh preparation before use is necessary.…”

Section: Azo Couplingmentioning

confidence: 99%

“…Electron-poor diazonium salts were shown to form more intensely colored products after fixed reaction times (6 min). Although no underlying kinetic study was undertaken and single points were used as read-outs, the authors concluded that electron-poor systems are the better analytical probes for rapid screenings …”

Section: Chromo- and Fluorogenic Probes For Phenolsmentioning

confidence: 99%

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Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

et al. 2023

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Many successful stories in enzyme engineering are based on the creation of randomized diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods that enabled their evaluation with high throughput are dominated by spectroscopic techniques due to their high speed and sensitivity. A large proportion of studies relies on fluorogenic substrates that mimic the chemical properties of the target or coupled enzymatic assays with an optical read-out that assesses the desired catalytic efficiency indirectly. The most reliable hits, however, are achieved by screening for conversions of the starting material to the desired product. For this purpose, functional group assays offer a general approach to achieve a fast, optical read-out. They use the chemoselectivity, differences in electronic and steric properties of various functional groups, to reduce the number of false-positive results and the analytical noise stemming from enzymatic background activities. This review summarizes the developments and use of functional group probes for chemoselective derivatizations, with a clear focus on screening for enzymatic activity in protein engineering.

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Section: Azo Couplingmentioning

confidence: 99%

Section: Chromo- and Fluorogenic Probes For Phenolsmentioning

confidence: 99%