Potential-controlled chromatography for the separation of amino acids and peptides

Abstract

Potential-controlled chromatography is introduced as a new technique for the separation of amino acids and peptides. The principle of potential-controlled chromatography depends on the use of electrically conductive material as the stationary phase of the chromatographic column. Thus from an electrochemical point of view the packed column can be regarded as a packed-bed electrode. The electrical potential of this stationary phase can be controlled by a potentiostat. The separation of amino acid and peptide molecules during their migration through the column depends on their own electric charge on the one hand and on the electrical potential of the stationary phase on the other. The chromatographic separation of some amino acids could be demonstrated.

Water desalination via capacitive deionization: what is it and what can we expect from it?

“…55 This concept can even be used to construct a cell for so called potential-controlled chromatography with improved separation (and detection) of charged species. 169,170 An alternative approach to achieve preferential removal of a certain ion utilizes a material with high selectivity towards one ionic species placed either on electrodes' surfaces or between electrodes' carbon particles. This approach has been demonstrated towards the preferential removal of nitrate over chloride and sulfate ions.…”

Adsorption of organic molecules on carbon surfaces: Experimental data and molecular dynamics simulation considering multiple protonation states

“…Moreover, a growing number of systems and processes use carbon-based sorbents. Some examples are fluidized bed electrode reactors [9,10], capacitive deionization devices and other preparative electrosorption techniques [11][12][13][14][15][16][17].…”

A Carbon Nanotube Packed Bed Electrode for Small Molecule Electrosorption: An Electrochemical and Chromatographic Approach for Process Description

“…Knizia et al [26] and Kocak et al [27] pushed the development of PCC further by extending the process from the analytical to the preparative scale. They employed a packed bed electrode of spherical, non-functionalized glassy carbon particles which had a relatively small specific surface area (<10 m 2 /g) [26,27]. Recently, Brammen et al [28] implemented multi-walled carbon nanotubes (MWCNTs; Baytubes C150P) as an innovative packed bed electrode material with high specific surface area (≈200 m 2 /g).Indeed, MWCNTs have already been introduced as a resin for typical gas or liquid chromatography applications [29][30][31][32][33], and as electrodes material in batteries and in sensor science [34][35][36][37].…”