Potential biochemical, genetic and molecular markers of deterioration advancement in seeds of oilseed rape (Brassica napus L.)

Boniecka, Justyna; Kotowicz, Karolina; Skrzypek, Edyta; Dziurka, Kinga; Rewers, Monika; Jędrzejczyk, Iwona; Wilmowicz, Emilia; Berdychowska, Julia; Dąbrowska, Grażyna B.

doi:10.1016/j.indcrop.2018.12.098

Cited by 23 publications

(26 citation statements)

References 82 publications

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“…A gradual loss of seed vigour resulting in the eventual loss of viability has been reported to characterise seed deterioration (Argerich and Bradford, 1989;Lehner et al, 2008). This may be attributed to various factors including deterioration-induced cellular, chemical and metabolic modifications (Bailly et al, 2008;Boniecka et al, 2019). Uncontrolled ROS accumulation during hydration of aged seeds inhibits radicle protrusion (germination) or causes seeds to develop into abnormal seedlings (Bailly et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Seeds gradually and continuously suffer post-harvest deterioration leading to quality loss during prolonged storage (Sahu et al, 2017). This process of ageing causes delayed germination, reduced vigour and eventual total viability loss (Boniecka et al, 2019). The higher the seed moisture level and temperature at which seeds are stored, the faster they lose viability (Simon, 1974).…”

Section: Introductionmentioning

confidence: 99%

“…Given the above, the identification of biochemical markers of oxidative stress has become a popular area of investigation in terms of seed deterioration (Murthy et al, 2003;Kim et al, 2010;Boniecka et al, 2019). These markers range from ROS and antioxidants (enzymic and nonenzymic) to products of oxidative processes (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Assessing levels of antioxidant protection are just as important as measuring oxidative injury when investigating oxidative stress in biological systems (Moran et al, 1994). In this regard, antioxidants including detoxifying enzymes such as superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), catalase (CAT) and ascorbate peroxidase (APX) are often used as indicators of seed deterioration (Kim et al, 2010;Yan et al, 2016;Boniecka et al, 2019) given their role in defending plant tissues against oxidative stress (Sahu et al, 2017;Wang et al, 2018). Seed ageing has also been shown to be accompanied by a decline in the activity of germination enzymes like a-amylase (Livesley and Bray, 1991;Ganguli and Sen-Mandi, 1993) and several studies have shown the involvement of bÀ1,3-glucanase in seed germination by promoting radicle protrusion (Leubnermetzger and Meins, 1999;Koornneef et al, 2002;Leubner-Metzger, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Effects of exogenous application of five antioxidants on vigour, viability, oxidative metabolism and germination enzymes in aged cabbage and lettuce seeds

Adetunji

Sershen

Varghese

et al. 2021

South African Journal of Botany

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Ageing induces modifications capable of compromising seed vigour and viability. This study investigated the effects of exogenous application of five antioxidants: ascorbic acid (AA), gallic acid (GA), reduced glutathione (GSH), trolox and glycerol, on physical and biochemical lesions induced by controlled deterioration (CD) in Brassica oleracea (cabbage) and Lactuca sativa (lettuce) seeds. The antioxidants were applied at 0.2, 0.4 and 0.6 mM to fresh seeds and seeds subjected to CD: 75% viability (P75), 50% viability (P50) and 25% viability (P25); deionised water (DW) served as control. Controlled deterioration resulted in more abnormal seedlings production in cabbage than in lettuce seeds. Additionally, CD increased electrical conductivity (EC) and protein carbonylation (PC), lowered antioxidant and germination enzymes activities in both species but increased lipid peroxidation in lettuce seeds only. Exogenous application of antioxidants enhanced seed viability of cabbage at P25 and lettuce at P50 and P25; seedling vigour of fresh, P75 and P25 cabbage; P50 and P25 lettuce seeds. Electrolyte conductivity and lipid peroxidation were reduced by AA, GA, glycerol, GSH and trolox in lettuce seeds, while PC was reduced by GA, glycerol and GSH in both species. Antioxidant and germination enzymes activities were heightened by GA, glycerol, GSH, and trolox in cabbage seeds; AA, GA, glycerol, GSH and trolox in lettuce seeds. While DW reduced PC in cabbage seeds, it reduced lipid peroxidation and heightened antioxidant and germination enzymes activities in lettuce seeds. The study resolved that CDinduced lesions differ in both species, and the exogenously applied antioxidants are beneficial in quelling injurious spin-offs of oxidative reactions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of exogenous application of five antioxidants on vigour, viability, oxidative metabolism and germination enzymes in aged cabbage and lettuce seeds

Adetunji

Sershen

Varghese

et al. 2021

South African Journal of Botany

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show abstract

“…As the storage time increases, seed vigor gradually decreases to zero (Redden and Partington, 2019). Since the degradation of seed vitality during storage is closely related to germination (Boniecka et al, 2019;Lv et al, 2016), changes in vigor inevitably affect the growth of seeds after sowing. In addition to germination rate, growth rate and maximum growth potential are also affected by storage time (Kholibrina et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

An Endeavor of ‘Deep-underground Agriculture’: Deep-underground Storage in a Gold Mine Impacts the Germination of Canola (Brassica Napus L.) Seeds

Wang

et al. 2021

Preprint

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Exploring and utilizing the agronomic potential of deep-underground is one of the ways to cope with the challenges of sudden environmental change on agriculture. Understanding the effects of environmental stresses on the morphological and physiological indicators of crop seeds after their storage deep-underground is crucial to developing and implementing strategies for agriculture in the deep-underground space. In this study, we stored canola seeds in envelopes or sealed packages at 0, 240, 690, and 1,410 m in a gold mine. Seeds in envelopes were retrieved at 42, 66, 90, and 227 days of storage, whereas seeds in sealed packages were retrieved at 66 and 227 days of storage. The germination tests were conducted to investigate the effects of storage depth, duration, and packing method on stored and non-stored seeds. Results showed that increased depth and duration led to decreased seed germination rate, with the germination and vigor indexes also descending to varying degrees. Increased hypocotyl length and biomass accumulation suggested that deep-underground environment had more significant compensatory effect on seed germination. For all indicators, the performance of seeds sealed in packages was superior to those stored in envelopes. Regression analysis showed that it was difficult to obtain the optimal value of each indicator simultaneously. The successful germination experiment foreshadowed the possibilities of deep-underground agriculture in the future.

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CRISPR/Cas-Based Precision Breeding of Oilseed Rape (Brassica napus L.) – Recent Improvements

Boniecka

2023

A Roadmap for Plant Genome Editing

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The seeds of oilseed rape (Brassica napus L.) are used in large-scale production of one of the most health-promoting plant oils in the food industry, as well as for animal feed and biofuel production. Thus, increasing the yield of this crop is of crucial economic and ecological importance. However, conventional breeding programs are slow, laborious and time-consuming. Hence, along with the discovery of the possibility to apply CRISPR/Cas technology to edit plant genomes and to accelerate the breeding process, much effort has been put into applying this technology to study specific genes and biosynthetic pathways, especially in species with many gene copies such as B. napus. Here, recent improvements in generating CRISPR/Cas-induced mutations in the B. napus genome, delivering CRISPR/Cas reagents into oilseed rape plant cells, fast-checking the efficiency of targeted mutagenesis of CRISPR/Cas reagents, and oilseed rape transformation and regeneration procedures are described. Finally, new applications of CRISPR/Cas tools in oilseed rape precision breeding are discussed, focusing mainly on applications verified in field.

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Potential biochemical, genetic and molecular markers of deterioration advancement in seeds of oilseed rape (Brassica napus L.)

Cited by 23 publications

References 82 publications

Effects of exogenous application of five antioxidants on vigour, viability, oxidative metabolism and germination enzymes in aged cabbage and lettuce seeds

Effects of exogenous application of five antioxidants on vigour, viability, oxidative metabolism and germination enzymes in aged cabbage and lettuce seeds

An Endeavor of ‘Deep-underground Agriculture’: Deep-underground Storage in a Gold Mine Impacts the Germination of Canola (Brassica Napus L.) Seeds

CRISPR/Cas-Based Precision Breeding of Oilseed Rape (Brassica napus L.) – Recent Improvements

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