Potential benefits of polysaccharides derived from marine alga Ulva lactuca against hepatotoxicity and nephrotoxicity induced by thiacloprid, an insecticide pollutant

Abstract

The present study aimed to evaluate the potential protective and antioxidant effect of polysaccharides (PS) extracted from Ulva lactuca against thiacloprid (THC) induced nephrotoxicity and hepatotoxicity. The antioxidant capacity of PS was tested in vitro using ABTS radical scavenging activity and plasmid DNA cleavage assays andin vivo on adult male rats treated for 30 days. Animals were allocated into four groups: control; THC (22.5 mg/kg); THC (22.5 mg/kg) + PS1 (100 mg/kg diet); and THC (22.5 mg/kg) + PS2 (200 mg/kg diet). The structural features of PS were determined by Fourier transformed infrared (FT‐IR), UV absorption peak detection, high performance liquid chromatography (HPLC) and gel permeation chromatography, and also functional properties were investigated. Overall, results indicated that THC increased significantly malondialdehyde, advanced oxidation protein products, glutathione levels, which is correlated with severe histological and plasmatic biochemical injuries in both liver and kidney tissues. However, cotreatment PS induced a significant protective and healing affects against the nephrotoxicity and hepatotoxcity induced by THC.

Astaxanthin Mitigates Thiacloprid-Induced Liver Injury and Immunotoxicity in Male Rats

“…Its application may constitute a potential hazard to animals and humans consuming contaminated food or water. The liver was suggested as the primary target organ for TCP [ 3 , 4 ], although nephrotoxicity [ 4 ], teratogenicity, and carcinogenicity [ 5 ] were also reported. Moreover, TCP impaired the development of both rabbit and mouse preimplantation embryos [ 6 ], induced oxidative stress, apoptosis, and genotoxicity in bovine lymphocytes [ 7 ], and produced DNA damage in human hepatocellular carcinoma (HepG2) cells [ 8 ].…”

Co-exposure to deltamethrin and thiacloprid induces cytotoxicity and oxidative stress in human lung cells

“…THIA (0.5–4 mM) decreased the viability of human neuroblastoma and human hepatocellular carcinoma cells after 24- and 48-h treatments (Şenyıldız et al, 2018). THIA (22.5 mg kg −1 ) for 30 days induced nephrotoxicity and hepatotoxicity by increasing MDA and decreasing GSH activities in the liver and kidney of rats (Kammoun et al, 2019). Oxidant-based DNA damage and decreased cell viability and proliferation were detected in bovine peripheral lymphocytes treated with THIA (10, 30, 60, 120, and 240 µg mL −1 ) for 4 h (Schwarzbacherová et al, 2019).…”

Ulvan, a Polysaccharide from Macroalga Ulva sp.: A Review of Chemistry, Biological Activities and Potential for Food and Biomedical Applications

“…Besides in vitro antioxidant tests, animals can be exposed to radicals, such as thiacloprid, and then treated with an extract [5,6]. In hypercholesterolemia mice, ulvan reduces oxidative stress by increasing the activity of antioxidant enzymes (110% for CAT, 77% for GPx, and 23% for SOD) and the level of nonenzymatic antioxidants (GSH).…”

“…Lahaye et al reported that the ulvan yield ranges from 8% to 29% of the dry weight of algae based on the extraction and purification methods [3]. It is built on two main repeating disaccharides, type A ulvanobiuronic acid 3-sulfate (A 3S ) and type B ulvanobiuronic acid 3-sulfate (B 3S ) [2][3][4][5][6][7][8]. Ulvan is widely used as a food additive and several studies have examined its therapeutic benefits [7][8][9].…”