Potential applications of antimicrobial peptides and their mimics in combating caries and pulpal infections

Mai, Sui; Mauger, Matthew T.; Niu, Li Na; Barnes, Jonathan; Kao, Solon; Bergeron, Brian E.; Ling, Jun; Tay, Franklin R.

doi:10.1016/j.actbio.2016.11.026

Cited by 106 publications

(84 citation statements)

References 209 publications

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“…Transmembrane pore formation, intracellular targeting and metabolic inhibition mechanisms (inhibition of microbial functional proteins, DNA and RNA synthesis) [14,15] Synthetic AMPs (e.g. ␤-17, human neutrophil peptides 1 and 2, histatins 5 and 8) Cationic compounds Chitosan Interaction between positively charged chitosan molecules and negatively charged bacterial cell membranes leads to disruption of cell membrane [16] Chlorhexidine Bind to negatively charged bacterial walls and disrupt cell walls Poly(-lysine)…”

Section: Antibioticsmentioning

confidence: 99%

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Jiao

Niu

et al. 2017

Progress in Polymer Science

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a b s t r a c tMicrobial infections affect humans worldwide. Many quaternary ammonium compounds have been synthesized that are not only antibacterial, but also possess antifungal, antiviral and anti-matrix metalloproteinase capabilities. Incorporation of quaternary ammonium moieties into polymers represents one of the most promising strategies for preparation of antimicrobial biomaterials. Various polymerization techniques have been employed to prepare antimicrobial surfaces with quaternary ammonium functionalities; in particular, syntheses involving controlled radical polymerization techniques enable precise control over macromolecular structure, order and functionality. Although recent publications report exciting advances in the biomedical field, some of these technological developments have also been accompanied by potential toxicological and antimicrobial resistance challenges. Recent evidenced-based data on the biomedical applications of antimicrobial quaternary ammonium-containing biomaterials that are based on randomized human clinical trials, the golden standard in contemporary medicinal science, are included in the present review. This should help increase visibility, stimulate debates and spur conversations within a wider scientific community on the implications and plausibility for future developments of quaternary ammonium-based antimicrobial biomaterials.Published by Elsevier B.V.

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Section: Antibioticsmentioning

confidence: 99%

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Jiao

Niu

et al. 2017

Progress in Polymer Science

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474

317

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“…Over the last decade, a significant leap in studies about control of oral pathogens by AMPs was observed [13]. So far, many natural or synthetic AMPs have shown inhibitory effects on growth of planktonic cells and biofilm of S. mutans in vitro , including defensins, Cathelicidin LL-37, Histatin 5, Human Lactoferrin [14], KSL [15], L-K6 [16], Bac8c [17], and C16G2 [18].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial peptide GH12 suppresses cariogenic virulence factors of Streptococcus mutans

Wang

Jiang

et al. 2018

Journal of Oral Microbiology

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Cariogenic virulence factors of Streptococcus mutans include acidogenicity, aciduricity, and extracellular polysaccharides (EPS) synthesis. The de novo designed antimicrobial peptide GH12 has shown bactericidal effects on S. mutans, but its interaction with virulence and regulatory systems of S. mutans remains to be elucidated. The objectives were to investigate the effects of GH12 on virulence factors of S. mutans, and further explore the function mechanisms at enzymatic and transcriptional levels. To avoid decrease in bacterial viability, we limited GH12 to subinhibitory levels. We evaluated effects of GH12 on acidogenicity of S. mutans by pH drop, lactic acid measurement and lactate dehydrogenase (LDH) assay, on aciduricity through survival rate at pH 5.0 and F1F0-ATPase assay, and on EPS synthesis using quantitative measurement, morphology observation, vertical distribution analyses and biomass calculation. Afterwards, we conducted quantitative real-time PCR to acquire the expression profile of related genes. GH12 at 1/2 MIC (4 mg/L) inhibited acid production, survival rate, EPS synthesis, and biofilm formation. The enzymatic activity of LDH and F1F0-ATPase was inhibited, and ldh, gtfBCD, vicR, liaR, and comDE genes were significantly downregulated. In conclusion, GH12 inhibited virulence factors of S. mutans, through reducing the activity of related enzymes, downregulating virulence genes, and inactivating specific regulatory systems.

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“…The study of the proteome of saliva and crevicular fluid has aided in discerning the bioactivity of a number of biomolecular components (proteins and peptides) present in the oral environment. More than 40 antimicrobial peptides and proteins are expressed in the oral cavity [12,13], and other proteins are known to have an active role in controlling the biomineralization processes on teeth. Statherin is a 43-residue multifunctional protein that is present in human saliva, and which can regulate the mineralization occurring in the oral cavity and tissues [14].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants

Govindharajulu

Chen

et al. 2017

IJMS

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The main clinical problems for dental implants are (1) formation of biofilm around the implant—a condition known as peri-implantitis and (2) inadequate bone formation around the implant—lack of osseointegration. Therefore, developing an implant to overcome these problems is of significant interest to the dental community. Chitosan has been reported to have good biocompatibility and anti-bacterial activity. An osseo-inductive recombinant elastin-like biopolymer (P-HAP), that contains a peptide derived from the protein statherin, has been reported to induce biomineralization and osteoblast differentiation. In this study, chitosan/P-HAP bi-layers were built on a titanium surface using a layer-by-layer (LbL) assembly technique. The difference in the water contact angle between consecutive layers, the representative peaks in diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), X-ray photoelectron spectroscopy (XPS), and the changes in the topography between surfaces with a different number of bi-layers observed using atomic force microscopy (AFM), all indicated the successful establishment of chitosan/P-HAP LbL assembly on the titanium surface. The LbL-modified surfaces showed increased biomineralization, an appropriate mouse pre-osteoblastic cell response, and significant anti-bacterial activity against Streptococcus gordonii, a primary colonizer of tissues in the oral environment.

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Potential applications of antimicrobial peptides and their mimics in combating caries and pulpal infections

Cited by 106 publications

References 209 publications

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Quaternary ammonium-based biomedical materials: State-of-the-art, toxicological aspects and antimicrobial resistance

Antimicrobial peptide GH12 suppresses cariogenic virulence factors of Streptococcus mutans

Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants

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