Potential application of urea-derived herbicides as cytokinins in plant tissue culture

Srinivasan, Malathi; Nachiappan, Vasanthi; Rajasekharan, Ram

doi:10.1007/bf02708412

Cited by 14 publications

(7 citation statements)

References 22 publications

(12 reference statements)

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“…Some PGRs are herbicides, including 2,4-D, TDZ, picloram and dicamba, are popular in plant tissue culture studies, and when added to medium at high concentrations, may kill plant tissues and cause developmental aberrations. TDZ is traditionally used as a herbicide in agriculture but became increasingly employed in plant tissue culture 17 . TDZ affects plant physiology, such as cellular, energy, nutrient, transport and metabolic alterations to the membrane, and in flowering Dendrobium , alters the endogenous levels of PGRs 18 .…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang

Ouyang

Chen

Lü

et al. 2016

Sci Rep

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An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid (IAA) could induce shoots when on basal Murashige and Skoog (MS) medium. BA and TDZ could induce more adventitious shoots (19.1 and 31.2/explant, respectively) than NAA (4.6/explant), IBA (5.7/explant) or IAA (6.4/explant). BA and TDZ at 5–10 μM could induce both shoots and somatic embryos. A higher concentration of TDZ (25 μM) induced only somatic embryos (39.8/explant). The same concentration of BA induced both adventitious shoots (23.6/explant) and somatic embryos (9.7/explant). Thus, somatic embryogenesis in this plant needs a high cytokinin concentration (BA; TDZ), as evidenced by histology. Somatic embryos germinated easily when left on the same media, but formed adventitious roots in two weeks on MS supplemented with 0.5 μM NAA, 0.5 μM IBA and 0.1% activated charcoal. Over 93% of plantlets survived following acclimatization and transfer to a mixture of sand and vermiculite (1:1, v/v) in trays.

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Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang

Ouyang

Chen

Lü

et al. 2016

Sci Rep

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“…Cytokinins play a central role in the regeneration of multiple shoots in many medicinal plant species [32,33] and sometimes in combination with auxins. Cytokinins trigger cell division and influence differentiation [34], while auxins are a major signal for apical dominance [35] and are responsible for elongation in phototropism and gravitropism [36]. In vitro plantlets will then be transferred to potting media during acclimatization, the final stage.…”

Section: Introductionmentioning

confidence: 99%

The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri

Suraya

Hakiman

2021

Plants

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Phyllanthus niruri (P. niruri) or Dukung Anak is a herbal plant in the Phyllanthaceae family that has been used traditionally to treat various ailments such as diabetes, jaundice, flu and cough. P. niruri contains numerous medicinal benefits such as anti-tumor and anti-carcinogenic properties and a remedy for hepatitis B viral infection. Due to its beneficial properties, P. niruri is overharvested and wild plants become scarce. This study was conducted to develop an appropriate in vitro culture protocol for the mass production of P. niruri. An aseptic culture of P. niruri was established followed by multiplication of explants using different types of basal medium and its strength and plant growth regulators manipulation. This study also established the induction of in vitro rooting utilizing various types and concentrations of auxin. Treatment of Clorox® with 30% concentration showed the lowest percentage (%) of contamination, 4.44% in P. niruri culture. Nodal segments of P. niruri were successfully induced in full-strength of Murashige and Skoog (MS) basal media with 2.33 number of shoots, 3.11 cm length of shoot and 27.91 number of leaves. In addition, explants in full-strength MS media without any additional cytokinin were recorded as the optimum results for all parameters including the number of shoots (5.0 shoots), the length of shoots (3.68 cm) and the number of leaves (27.33 leaves). Treatment of 2.5 µM indole-3-butyric acid (IBA) showed the highest number of roots (17.92 roots) and root length (1.29 cm). Rooted explants were transferred for acclimatization, and the plantlet showed over 80% of survival rate. In conclusion, plantlets of P. niruri were successfully induced and multiplied via in vitro culture, which could be a step closer to its commercialization.

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“…Ureas are a very important class of carbonyl compounds which find extensive application as agrochemicals, dyes for cellulose fibers, antioxidants in gasoline, resin precursors, and pharmaceuticals [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Phosgene-free synthesis of 1,3-diphenylurea via catalyzed reductive carbonylation of nitrobenzene

Vavasori

Ronchin

2012

Pure and Applied Chemistry

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1,3-Diphenylurea (DPU) has been proposed as a synthetic intermediate for phosgene-free synthesis of methyl N-phenylcarbamate and phenyl isocyanate, which are easily obtained from the urea by reaction with methanol. Such an alternative route to synthesis of carbamates and isocyanates necessitates an improved phosgene-free synthesis of the corresponding urea. In this work, it is reported that Pd(II)-diphosphine catalyzed reductive carbonylation of nitrobenzene in acetic acid (AcOH)-methanol proceeds in high yield and selectivity as a one-step synthesis of DPU. We have found that the catalytic activity and selectivity of this process depends on solvent composition and on the bite angle of the diphosphine ligands. Under optimum reaction conditions, yields in excess of 90 molar % and near-quantitative selectivity can be achieved.

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Potential application of urea-derived herbicides as cytokinins in plant tissue culture

Cited by 14 publications

References 22 publications

Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang

Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia W. T. Wang

The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri

Phosgene-free synthesis of 1,3-diphenylurea via catalyzed reductive carbonylation of nitrobenzene

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