Potential application of two cobalt (III) Schiff base complexes in cancer chemotherapy: Leads from a study using breast and lung cancer cells

Balakrishnan, Ganesh; Ambika, Subramanian; Balakrishnan, Kalpana; Ramya, Venkatesan; Kadalmani, Balamuthu; Vimala, R.T.V.; Akbarsha, Mohammad Abdulkader

doi:10.1016/j.tiv.2021.105201

Cited by 31 publications

(13 citation statements)

References 46 publications

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“…DMSO 0.02% was used as a negative control. Apoptosis or necrosis was determined based upon the cytoplasmic organization and nuclear morphology ( Gowdhami et al, 2021a , b ; Ramya et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

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Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

et al. 2022

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It has been reported that coconut oil supplementation can reduce neuroinflammation. However, coconut oils are available as virgin coconut oil (VCO), crude coconut oil (ECO), and refined coconut oil (RCO). The impact of coconut oil extraction process (and its major fatty acid component lauric acid) at cellular antioxidant level, redox homeostasis and inflammation in neural cells is hitherto unexplained. Herein, we have shown the antioxidant levels and cellular effect of coconut oil extracted by various processes in human neuroblastoma cells (SH-SY5Y) cultured in vitro. Results indicate VCO and ECO treated cells displayed better mitochondrial health when compared to RCO. Similar trend was observed for the release of reactive oxygen species (ROS), key oxidative stress response genes (GCLC, HO-1, and Nqo1) and inflammatory genes (IL6, TNFα, and iNOS) in SH-SY5Y cells. Our results signified that both VCO and ECO offer better neural health primarily by maintaining the cellular redox balance. Further, RCO prepared by solvent extraction and chemical refining process lacks appreciable beneficial effect. Then, we extended our study to find out the reasons behind maintaining the cellular redox balance in neuroblastoma cells by VCO and ECO. Our GC-MS results showed that lauric acid (C14:0) (LA) content was the major difference in the fatty acid composition extracted by various processes. Therefore, we evaluated the efficacy of LA in SH-SY5Y cells. The LA showed dose-dependent effect. At IC50 concentration (11.8 μM), LA down regulated the oxidative stress response genes and inflammatory genes. The results clearly indicate that the LA inhibited the neuroinflammation and provided an efficient cellular antioxidant activity, which protects the cells. The efficiency was also evaluated in normal cell line such as fibroblasts (L929) to cross-validate that the results were not false positive. Different concentration of LA on L929 cells showed high compatibility. From our observation, we conclude that VCO and ECO offers better cellular protection owing to their powerful antioxidant system. Therefore, we advocate the inclusion of either VCO and/or ECO in the diet for a healthy lifestyle.

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Section: Methodsmentioning

confidence: 99%

“…The normal and abnormal nuclear morphologies were observed and recorded. Experiments were performed in triplicate ( Gowdhami et al, 2021a ).…”

Section: Methodsmentioning

confidence: 99%

Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

et al. 2022

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“…In this study, we explored the anticancer potential of cobalt (III) Schiff base complexes against human cervical and liver cancer cell lines, as well as validating our previous ndings with A549 and MCF7 cells (Gowdhami et al, 2021). In order to con rm the cell cytotoxicity, various experiments on HeLa and HepG2 cells were conducted with the cobalt (III) Schiff base complexes.…”

Section: Discussionmentioning

confidence: 73%

“…The molecular processes behind the anticancer capability of cobalt (III) complexes against HeLa and HepG2 cell lines were investigated, and the results proved that the compound had the DNA intercalating and groove binding characteristics (Ambika et al, 2019). Our recent ndings demonstrated the antiproliferative e ciency of the cobalt (III) Schiff base complexes 1 and 2 against human lung cancer cell lines (A549) and breast cancer cells (MCF-7) (Gowdhami et al, 2021).…”

Section: Introductionmentioning

confidence: 74%

Influence of cobalt (III) Schiff base complexes on in-vitro anti-proliferative, oxidative stress and gene expression analysis in HeLa and HepG2 cell lines

Balakrishnan

R.T.V

Asik

et al. 2022

Preprint

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The development of new medicines for the successful treatment of cervical and liver malignancies is critical in order to address the disadvantages of current chemotherapeutics, such as increased resistance. Metal ion-based chemical complexes have recently emerged as a prominent method for cancer therapy. As a result, the study aims to create novel anticancer medicines based on leads acquired through combinatorial chemistry of metal complexes. Cobalt (III) Schiff base trans- [Co(salen)(DA)2](ClO4) (complex 1) and trans-[Co (salophen)(DA)2](ClO4) (complex 2)] where, the salen and salopen were N, N′-bis(salicylidene) ethylenediamine, and N, N′-bis(salicylidene)-1,2- phenylenediamine, DA: dodecylamine) were synthesized as an alternative to the existing drugs, and their cytotoxic effect were evaluated against human cervical (HeLa) and liver cancer cell lines (HepG2) using MTT viability assay, and apoptotic morphological staining which including Acridine Orange/Ethidium Bromide (AO/EB), Hoechst 33528, Annexin V-Cy3 assay, JC-1 staining Comet assay, and reactive oxygen species (ROS) assay. Further the apoptosis was confirmed with immunocytochemistry and real-time reverse transcription-polymerase chain reaction (RT-PCR). The results indicated that cobalt (III) complexes reduced the viability of the HeLa and HepG2 cell lines at their half minimum lethal doses, with cell death induced mainly via apoptotic pathway. Hence, the cobalt complexes 1 and 2 could be act as an effective drug for cervical and liver cancers.

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“…Literatüre surveys have shown that Schiff Bases and their derivatives are very important compounds both in healty fields as bioactive molecule and technology fields as catalist, sensor (Antony et al, 2019;Ashraf et al, 2021). Especially, metal complexs of Shchiff Bases have tested antibacterial (Chohan et al, 2001), anti-fungal (Chohan et el., 2010) herbidical and anticancer (Duff et al, 2012;Kaczmarek et al,2018;Gowdhami et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Calculation of Some Theoretical Properties of 3-(p-Methoxybenzyl)-4-(4-Hydroxybenzylidenamino)-4,5-Dihydro-1H-1,2,4-Triazol-5-One with DFT

BOY¹,

Gul²,

Haydar³

2022

EPSTEM

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In this study, the quantum chemical computations of 3-(p-methoxybenzyl)-4-(4-hydroxybenzylidenamino)-4,5-dihydro-1H-1,2,4-triazol-5-one that is a triazole derivaties were calculated using DFT method in the 6-311++G(d,p) basis set. Firstly, the molecule was optimized for the most stable positions of the atoms. The nuclear magnetic rezonans (13C-NMR and 1H-NMR) data were calculated with the GIAO method in the Gaussian O9W package program and the results were compared with the experimental values in the literature. Furthermore, theoretical infrared (IR) vibration frequencies values which were scaled with certain scala factor were obtained using the Veda 4 program. In this study, compound’s thermodynamic parameters such as (entropy S0, heat capacity CV0 and enthalpy H0), geometric properties (bond angle and length), electronic parameters (global hardness (η), electron affinity (A), electronegativity (χ), softness (σ) and ionization potential (I), ELUMO-EHOMO energy gap, HOMO-LUMO energy), dipole moment, mulliken atomic charges were also studied.

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Potential application of two cobalt (III) Schiff base complexes in cancer chemotherapy: Leads from a study using breast and lung cancer cells

Cited by 31 publications

References 46 publications

Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

Dual Roles of Coconut Oil and Its Major Component Lauric Acid on Redox Nexus: Focus on Cytoprotection and Cancer Cell Death

Influence of cobalt (III) Schiff base complexes on in-vitro anti-proliferative, oxidative stress and gene expression analysis in HeLa and HepG2 cell lines

Calculation of Some Theoretical Properties of 3-(p-Methoxybenzyl)-4-(4-Hydroxybenzylidenamino)-4,5-Dihydro-1H-1,2,4-Triazol-5-One with DFT

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