Potential application of developed methanogenic microbial consortia for coal biogasification

Fuertez, John; Córdoba, Gloria; McLennan, John; Adams, D.; Sparks, Taylor D.

doi:10.1016/j.coal.2018.02.013

Cited by 20 publications

(15 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As illustrated, the gas production process can be roughly divided into three stages: the first stage (0–40 days) of slow growth, the second stage (40–60 days) of rapid growth, and the third stage (60–90 days) of restrained growth. This observation is consistent with the conclusion drawn by Fuertez et al (2018). In the first stage, the microflora needed to adapt to the new environment and their number was fewer, so less gas was produced (Take group C as an example, the cumulative methane production was 4.2 mg/g).…”

Section: Resultssupporting

confidence: 90%

“…According to the analysis results of bacteria, the participating bacteria mainly included Firmicutes, Proteobacteria, Bacteroidetes, and a small amount of Synergistetes in the whole process of gas production. They are very common in the study and cultivation of CBM (Fuertez et al, 2018). The Firmicutes microorganisms mainly participated in the generation of some mixed acids, alcohols, and neutral substances, it plays an important role in the degradation of coal and contributes to the degradation of coal into aromatic compounds, aliphatic compounds and alkanes (Colosimo et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…were found in the coal seam water of Cook Bay, Alaska, United States (Dawson et al, 2012). Fuertez et al (2018) found that Methanofollis and Methanobacterium were dominant in the optimized methane-producing bacteria solution. Although the microbial structure of CBM is extensively studied, the relationship between the increase in methane production and microbial flora, especially the reason why ethanol stimulates the increase in methane yield, is seldom reported.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Alteration of Methanogenic Archaeon by Ethanol Contribute to the Enhancement of Biogenic Methane Production of Lignite

et al. 2019

View full text Add to dashboard Cite

Bioconverting coal to methane is a green and environmental friendly method to reuse waste coal. In this study, heterologous bacteria were used for the gas-producing fermentation of lignite under laboratory conditions, simultaneously, different concentrations of ethanol added into the culture to investigate the effect of ethanol on gas production and microbial flora structure. Results show that when the ethanol concentration was 1%, the best methanogenesis was achieved at 44.86 mL/g, which was twice the gas production of 0% ethanol. Before and after gas fermentation, the composition and structure of the coal changed, the volatile matter and fixed carbon increased, and the ash decreased. The absorbance value at characteristic peaks of all functional groups decreased, new peaks were generated at 2,300/cm, and the peak value disappeared at 3,375/cm. Thus, microorganisms interacted with coal, consumed it, and produced new materials. The microbial flora changes during gas production were tracked in real time. 0.5 and 1% ethanol did not obviously change the bacterial communities but strongly influenced the archaeon communities, thereby changed the methane production pathway. In the absence of ethanol, Methanosarcina was continuously increasing with the extension of fermentation time, this pathway was the nutrient type of acetic acid. When ethanol was added, Methanobacterium gradually increased, the pathway was mainly hydrotropic type. In summary, adding ethanol can increase the coalbed methane production, change the structure and composition of coal, and facilitate the interaction of microbe with coal. Therefore, the methanogenic archaeon changes could help improve the methane-producing ability of lignite in the presence of ethanol.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Alteration of Methanogenic Archaeon by Ethanol Contribute to the Enhancement of Biogenic Methane Production of Lignite

et al. 2019

View full text Add to dashboard Cite

show abstract

“…During the cultivation period, major OTUs (>1% in any of the sponge carrier libraries) were affiliated with the phyla Proteobacteria , Firmicutes , Chloroflexi , Actinobacteria , Bacteroidetes , Spirochaetes , Tenericutes , Ignavibacteriae , and SBR1093. The phylum-level community composition was similar to those reported in the previous molecular studies of methanogenic communities in coal environments 38–42 and enriched communities from coal and its associated water 43–47 . However, the detailed composition of microbial community members enriched in our reactor at below the phylum level differed from the previously reported compositions, potentially due to the different sources of the inoculum and enrichment conditions (e.g., marine vs. terrestrial).…”

Section: Discussionsupporting

confidence: 83%

Cultivable microbial community in 2-km-deep, 20-million-year-old subseafloor coalbeds through ~1000 days anaerobic bioreactor cultivation

Imachi

Tasumi

Takaki

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Recent explorations of scientific ocean drilling have revealed the presence of microbial communities persisting in sediments down to ~2.5 km below the ocean floor. However, our knowledge of these microbial populations in the deep subseafloor sedimentary biosphere remains limited. Here, we present a cultivation experiment of 2-km-deep subseafloor microbial communities in 20-million-year-old lignite coalbeds using a continuous-flow bioreactor operating at 40 °C for 1029 days with lignite particles as the major energy source. Chemical monitoring of effluent samples via fluorescence emission-excitation matrices spectroscopy and stable isotope analyses traced the transformation of coalbed-derived organic matter in the dissolved phase. Hereby, the production of acetate and 13C-depleted methane together with the increase and transformation of high molecular weight humics point to an active lignite-degrading methanogenic community present within the bioreactor. Electron microscopy revealed abundant microbial cells growing on the surface of lignite particles. Small subunit rRNA gene sequence analysis revealed that diverse microorganisms grew in the bioreactor (e.g., phyla Proteobacteria, Firmicutes, Chloroflexi, Actinobacteria, Bacteroidetes, Spirochaetes, Tenericutes, Ignavibacteriae, and SBR1093). These results indicate that activation and adaptive growth of 2-km-deep microbes was successfully accomplished using a continuous-flow bioreactor, which lays the groundwork to explore networks of microbial communities of the deep biosphere and their physiologies.

show abstract

“…Significant volumes of litter accumulated near poultry farms are practically not utilized, since poultry farms do not have available technologies and equipment for the beneficial use of such litter quantities. However, poultry litter is raw material to obtain high-quality organic fertilizers, biogas [4][5][6][7]. Thus, the most urgent task is the improvement and implementation of environmentally friendly methods of processing litter and manure [1].…”

Section: Introductionmentioning

confidence: 99%

Processing of poultry farm waste by microbial conversion

et al. 2020

View full text Add to dashboard Cite

show abstract

Potential application of developed methanogenic microbial consortia for coal biogasification

Cited by 20 publications

References 84 publications

Alteration of Methanogenic Archaeon by Ethanol Contribute to the Enhancement of Biogenic Methane Production of Lignite

Alteration of Methanogenic Archaeon by Ethanol Contribute to the Enhancement of Biogenic Methane Production of Lignite

Cultivable microbial community in 2-km-deep, 20-million-year-old subseafloor coalbeds through ~1000 days anaerobic bioreactor cultivation

Processing of poultry farm waste by microbial conversion

Contact Info

Product

Resources

About