Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells

Nh, Kang; Ka, Hwang; Su, Kim; Yb, Kim; Sh, Hyun; Jeung, Eui‐Bae; Choi, Kyoung-Seong

doi:10.1038/cgt.2012.30

Cited by 73 publications

(66 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The conditioned medium from rat AECs contains soluble factors capable at inhibiting the proliferation rate of tumor cell lines from four different origins, in agreement with earlier reports using human AECs [44, 45]. Rat AEC-CM was very effective at reducing HepG2 cell proliferation while exhibiting lower potency, but still significant, against PANC-1, C6, and B16F10 tumor cells.…”

Section: Discussionsupporting

confidence: 89%

“…Some cancer cell lines are also more sensitive than others to the inhibitory effects of human AECs [46]. Here, further investigation into the mechanisms implicated in the antiproliferative effects of AEC-CM revealed the presence of soluble factors that interfere with cell cycle controls in tumor cells [47]. Indeed, an increase in the number of cells blocked in the G0/G1 phase of the cell cycle was observed with most of the cell lines tested (HepG2, B16F10 and PANC-1), in agreement with a previous study [44], while C6 cells were mostly accumulating in the S phase in response to AEC-CM treatment.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence

et al. 2016

View full text Add to dashboard Cite

Amniotic epithelial cells (AECs) are a class of fetal stem cells that derives from the epiblast and resides in the amnion until birth. AECs are suitable candidates for regenerative medicine because of the ease of collection, their low immunogenicity and inability to form tumors after transplantation. Even though human AECs have been widely investigated, the fact remains that very little is known about AECs isolated from rat, one of the most common animal models in medical testing. In this study, we showed that rat AECs retained stemness properties and plasticity, expressed the pluripotency markers Sox2, Nanog, and Oct4 and were able to differentiate toward the osteogenic lineage. The addition of conditioned medium collected from rat AECs to lipopolysaccharide-activated macrophages elicited anti-inflammatory properties through a decrease of Tnfa expression and slowed tumor cell proliferation in vitro and in vivo. The senescence-associated secretory phenotype was also significantly lower upon incubation of senescent human IMR-90 fibroblast cells with conditioned medium from rat AECs. These results confirm the potential of AECs in the modulation of inflammatory mechanisms and open new therapeutic possibilities for regenerative medicine and anti-aging therapies as well.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The utilization of stem cell transplantation is now being considered an anticancer therapy along with surgery, chemotherapy, and radiotherapy. Non-engineered and engineered stem cells have a number of features that make them useful as gene delivery vehicles in anticancer protocols (Kang et al, 2012a). In a breast cancer model, AECs reduce tumor progression and tumor size without compromising the normal breast tissue (Kang et al, 2012b).…”

Section: Anti-tumor Effects Of Aecsmentioning

confidence: 99%

“…Non-engineered and engineered stem cells have a number of features that make them useful as gene delivery vehicles in anticancer protocols (Kang et al, 2012a). In a breast cancer model, AECs reduce tumor progression and tumor size without compromising the normal breast tissue (Kang et al, 2012b). There is evidence that AECs inhibit tumor growth through the release of soluble molecules responsible for cell cycle blockade and/or apoptosis induction.…”

Section: Anti-tumor Effects Of Aecsmentioning

confidence: 99%

Amniotic Epithelial Cells: A New Tool to Combat Aging and Age-Related Diseases?

Germanio

Bernier

Cabo

et al. 2016

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

The number of elderly people is growing at an unprecedented rate and this increase of the aging population is expected to have a direct impact on the incidence of age-related diseases and healthcare-associated costs. Thus, it is imperative that new tools are developed to fight and slow age-related diseases. Regenerative medicine is a promising strategy for the maintenance of health and function late in life; however, stem cell-based therapies face several challenges including rejection and tumor transformation. As an alternative, the placenta offers an extraordinary source of fetal stem cells, including the amniotic epithelial cells (AECs), which retain some of the characteristics of embryonic stem cells, but show low immunogenicity, together with immunomodulatory and anti-inflammatory activities. Because of these characteristics, AECs have been widely utilized in regenerative medicine. This perspective highlights different mechanisms triggered by transplanted AECs that could be potentially useful for anti-aging therapies, which include: Graft and differentiation for tissue regeneration in age-related settings, anti-inflammatory behavior to combat “inflammaging,” anti-tumor activity, direct lifespan and healthspan extension properties, and possibly rejuvenation in a manner reminiscent of heterochronic parabiosis. Here, we critically discuss benefits and limitation of AECs-based therapies in age-related diseases.

show abstract

“…In particular, bone-marrow-derived MSCs have now been used clinically to repair bone defects [4,5]. In recent years, different studies have showed that human amniotic fluid provides a good and more easily accessible source of MSCs that show very low risk of tumour development, and no evident antigenicity [6,7]. These human amniotic fluid MSCs (huAFMSCs) can differentiate into osteoblastic cells [8], and when seeded onto scaffolds and cultured in osteogenic inductive medium, they support the formation of highly mineralised tissue after implantation in animal models [9][10][11][12] Bone regeneration after traumatic or degenerative damage requires interactions between cells and growth factors and the extracellular matrix.…”

Section: Introductionmentioning

confidence: 99%

Calcitonin-Induced Effects on Amniotic Fluid-Derived Mesenchymal Stem Cells

Morabito

D’Alimonte²,

Pierdomenico³

et al. 2015

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Mesenchymal stem cells from human amniotic fluid (huAFMSCs) can differentiate into multiple lineages and are not tumorigenic after transplantation, making them good candidates for therapeutic purposes. The aim was to determine the effects of calcitonin on these huAFMSCs during osteogenic differentiation, in terms of the physiological role of calcitonin in bone homeostasis. Methods: For huAFMSCs cultured under different conditions, we assayed: expression of the calcitonin receptor, using immunolabelling techniques; proliferation and osteogenesis, using colorimetric and enzymatic assays; intracellular Ca²⁺ and cAMP levels, using videomicroscopy and spectrophotometry. Results: The calcitonin receptor was expressed in proliferating and osteo-differentiated huAFMSCs. Calcitonin triggered intracellular Ca²⁺ increases and cAMP production. Its presence in cell medium also induced dose-dependent inhibitory effects on proliferation and increased osteogenic differentiation of huAFMSCs, as also indicated by enhancement of specific markers and alkaline phosphatase activity. Conclusions: These data show that huAFMSCs represent a potential osteogenic model to study in-vitro cell responses to calcitonin (and other members of the calcitonin family). This leads the way to the opening of new lines of research that will add new insight both in cell therapies and in the pharmacological use of these molecules.

show abstract

Potential antitumor therapeutic strategies of human amniotic membrane and amniotic fluid-derived stem cells

Cited by 73 publications

References 69 publications

Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence

Conditioned medium derived from rat amniotic epithelial cells confers protection against inflammation, cancer, and senescence

Amniotic Epithelial Cells: A New Tool to Combat Aging and Age-Related Diseases?

Calcitonin-Induced Effects on Amniotic Fluid-Derived Mesenchymal Stem Cells

Contact Info

Product

Resources

About