Potential antioxidant and anti-inflammatory effects of Hyphaena thebaica in experimentally induced inflammatory bowel disease

Abstract

Ulcerative colitis and Crohn's disease are chronic recurrent inflammatory bowel disease (IBD) of unknown origin. Oxidative stress is believed to be a key factor in the pathogenesis of the mucosal damage in IBD. The aqueous extract of Hyphaena thebaica fruits showed an antioxidant activity; this is due to the substantial amount of their water-soluble phenolic contents. This study was undertaken to study the effect of H. thebaica in experimentally induced IBD. Sprague Dawley rats were pretreated orally for 5 days with distilled water in control, H. thebaica 500 mg/kg in acetic acid + H. thebaica group and 5-aminosalisylic acid (5-ASA) 100 mg/kg in acetic acid + 5ASA groups. Colitis was induced by transrectal administration of 4% acetic acid on 5th day. All the animals were sacrificed with ether overdose 48 h after colitis induction, and 10 cm colon segment was resected from distal end. Colon was weighed (for disease activity index) and scored macroscopically and microscopically after histological staining. Biochemical assessments included myeloperoxidase (MPO) and tissue catalase (CAT), glutathione (GSH) and superoxide dismutase (SOD) measurements. H. thebaica showed significant prevention of increase in colon weight and disease activity index along with decrease in macroscopic and microscopic lesion score as compared to control group. Significant improvement was observed in the levels of MPO, CAT and SOD, except GSH. However, the effect of H. thebaica was significantly less than 5-ASA. H. thebaica at 500 mg/kg showed significant amelioration of experimentally induced IBD, which may be attributed to its antioxidant and anti-inflammatory properties.

Biological Activities of the Doum Palm (Hyphaene thebaica L.) Extract and Its Bioactive Components

“…As the plant H. thebaica is thought to possess anti-inflammatory and antioxidant properties, proving its role in the management of experimentally induced IBD [27]. It showed significant amelioration of experimentally induced IBD, which may be attributed to its antioxidant and anti-inflammatory properties [57]. The previous studies found that the weight and volume of contents of granuloma in inflammation were decreased after treatment with doum extract.…”

Hyperhomocysteinemia Lead to Transmural Inflammation of Colon and Increase Severity of Disease in Acetic Acid-Induced Colitis in Rat

“…Acetic acid induced colitis model is similar to human ulcerative colitis [30], it induces non-transmural inflammation, micosa edema, massive necrosis and neutrophil infiltration of mucosa and submucosa layers [31]. Intrarectal administration of acetic acid leads to protonation and migration of acetic acid molecule into colonic flora after internalization to produce protons leading to epithelial denudation and neutrophil infiltration [32].…”

Effect of co-administration of probiotics with polysaccharide based colon targeted delivery systems to optimize site specific drug release

“…Supernatant (0.1 ml) of each centrifuged sample was made up to 3 ml with phosphate buffer (pH 6.0) which contained 0.0005% v/v of H 2 O 2 . The change in absorbance per minute was measured spectrophotometrically (Shimadzu UV 1800, Japan), at 460 nm [55,56].…”