Potential Antioxidant Activity of Kedondong Leaves (Spondias dulcis Forst.) Using DPPH Method (1,1-Diphenyl-2- Pikril Hydrazil)

Abstract

Background: Antioxidants are substances that can reduce free radicals to protect the body's biological systems from adverse effects arising from processes or reactions that cause excess oxidants. Kedondong leaves (Spondias dulcis Forst.) contain flavonoids, tannins, and alkaloids, which have the potential to act as antioxidants. Objective: To determine the antioxidant activity of ethyl acetate and 95% ethanol extracts from kedondong leaves. Methods: The antioxidant activity was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method, a free radical stable in an aqueous solution. Each extract was tested for its antioxidant activity with a comparison compound, vitamin C, using a UV-Vis spectrophotometer. The results of the antioxidant activity test revealed the IC50 (inhibitory concentration) value, namely the concentration of antioxidant compounds capable of inhibiting DPPH free radical activity by 50%. Result: The ethyl acetate extract has weak antioxidant activity with an IC50 value of 194.123 ppm, the 95% ethanol extract has very weak antioxidant activity with an IC50 value of 553.3694 ppm, and vitamin C, as a comparison, has very strong antioxidant activity with an IC50 value of 4.7805 ppm. Conclusion: Kedondong leaves have potential antioxidant activity but are very small.