Potential anti-gout properties of Wuwei Shexiang pills based on network pharmacology and pharmacological verification

Bai, Lijie; Wu, Cheng-Hsin; Lei, Shuhui; Zou, Min; Wang, Shengjun; Zhang, Zhongyun; Bao, Zilu; Ren, Zhaoxiang; Liu, Kaiqun; Ma, Qianjiao; Ou, Hongyue; Lan, Zhou; Wang, Qian; Chen, Lvyi

doi:10.1016/j.jep.2023.116147

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…To further reveal the mechanism of interactions between intersectional target proteins, overlapping genes corresponding to each disease were identified and imported into the STRING website for the construction of PPI networks (Figures 4A, 5A, 6A, and 7A) and network analysis. Based on the degree value, 24,28,31 for the treatment of CG with CSF, the targets with the top 10 connectivity ranks were PIK3CA, STAT3, SRC, MAPK1, TP53, MAPK8, AKT1, VEGFA, TNF, and PTGS2, indicating that they played a key role in the overall network. Similarly, for the treatment of FD, the top 10 targets were PIK3CA, PIK3R1, TP53, STAT3, AKT1, SRC, VEGFA, PTGS2, ESR1, and TNF; the top 10 targets for the treatment of PU were STAT3, PIK3CA, MAPK1, EGFR, VEGFA, HRAS, TP53, AKT1, TNF, and PTGS2; and those for depression were MAKP1, STAT3, HSP90AA1, ESR1, AKT1, PTGS2, APP, VEGFA, MAPK14, and ESR1.…”

Section: Resultsmentioning

confidence: 99%

Section: Identification Of Absorbable Compounds In Csfmentioning

confidence: 99%

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Quality assessment and Q‐markers discovery in Citri Sarcodactylis Fructus by integrating serum pharmacochemistry and network pharmacology

Li,

Zhang,

Liu

et al. 2024

Phytochemical Analysis

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IntroductionCitri Sarcodactylis Fructus (CSF), a common fruit and traditional Chinese medicine (TCM), has been hindered in its further development and research owing to the lack of comprehensive and specific quality evaluation standards.ObjectiveThis study aimed to establish clear TCM quality standards related to the therapeutic mechanisms of CSF and to provide a basis for subsequent research and development.MethodsUltra‐high performance liquid chromatography coupled with hybrid quadrupole‐orbitrap high‐resolution mass spectrometry (UPLC‐Q‐orbitrap HRMS) technology was used to comprehensively identify CSF components and explore their absorbance levels in rat serum. Network pharmacology research methods were employed to investigate the potential mechanisms of action of the identified components in the treatment of major clinical diseases. Subsequently, a combination of HPLC chromatographic fingerprinting for qualitative analysis and multi‐index content determination was used to evaluate the detectability of the identified quality markers (Q‐markers).ResultsTwenty‐six prototype components were tentatively characterized in rat serum. Network pharmacology analysis showed six effective components, namely 7‐hydroxycoumarin, isoscopoletin, diosmin, hesperidin, 5,7‐dimethoxycoumarin, and bergapten, which played important roles in the treatment of chronic gastritis, functional dyspepsia, peptic ulcer, and depression and were preliminarily identified as Q‐markers. The results of content determination in 15 batches of CSF indicated significant differences in the content of medicinal materials from different origins. However, compared with the preliminarily determined Q‐markers, all six components could be measured and were determined as Q‐markers of CSF.ConclusionThe chemical Q‐markers obtained in this study could be used for effective quality control of CSF.

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Section: Resultsmentioning

confidence: 99%

Section: Identification Of Absorbable Compounds In Csfmentioning

confidence: 99%

Quality assessment and Q‐markers discovery in Citri Sarcodactylis Fructus by integrating serum pharmacochemistry and network pharmacology

Li,

Zhang,

Liu

et al. 2024

Phytochemical Analysis

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“…Wuwei-Shexiang pills, formulated with Acorus calamus L., Moschus berezovskii Flerov, Aconitum carmichaelii Debeaux., Aucklandia lappa (Decne) Decne., and Terminalia chebula Retz., exhibits the ability to alleviate ankle joint swelling and diminish TNF-α and IL-1β concentrations within MSU-induced mice air pouch lavage fluid. This is accomplished by restraining the levels of p-p38/p38 through the modulation of the MAPK signaling cascade, thus effectively mitigating gout-related inflammation ( Bai et al, 2023 ).…”

Section: Signal Pathways Of Tcm Modulating Gout Pathological Progressionmentioning

confidence: 99%

TCM and related active compounds in the treatment of gout: the regulation of signaling pathway and urate transporter

Sun,

Yang,

Sun

et al. 2023

Front. Pharmacol.

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Gout represents a metabolic ailment resulting from the accumulation of monosodium urate crystals within joints, causing both inflammation and, harm to tissues. The primary contributor to gout’s emergence is an elevated presence of serum urate, which is under the regulation of kidney and, gut urate transporters. Mitigating this risk factor is crucial for averting gout’s onset. Several treatments rooted in TCM and related active compounds have demonstrated efficacy in managing gout, skillfully regulating serum uric acid (UA) levels and curbing inflammation’s progression. This analysis compiles key foundational research concerning the molecular signaling pathways and UA transporters linked to gout, under the regulation of TCM. The focus includes individual botanical drug, active compounds, and TCM formulations, which have been consolidated and examined in this overview. The primary keywords chosen were “gout, hyperuricemia, gouty arthritis, traditional Chinese medicine, Chinese botanical drug, medicinal botanical drug, and natural plant”. Various relevant literature published within the last 5 years were gathered from electronic databases, including PubMed, Web of Science, CNKI, and others. The findings revealed that TCM has the capacity to modulate various signaling pathways, including MAPK, NF-κB, PI3K/Akt, NLRP3 and JAK/STAT. Additionally, it impacts UA transporters like URAT1, GLUT9, ABCG2, as well as OATs and OCTs, thereby contributing to gout treatment. TCM helps maintain a balanced inflammatory interaction and facilitates UA excretion. This study enhances our understanding of TCM’s anti-gout mechanisms and introduces novel perspectives for establishing the clinical significance and future prospects of TCM-based gout treatment.

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“…Therefore, clarifying the changing characteristics of metabolites in GN and treating metabolic disorders may be an important objective. Metabonomics is increasingly used to diagnose diseases, investigate the pathogenesis of diseases, identify new therapeutic targets, personalize drug therapy and examine the phenotypic differences of responses to small molecules across individuals (Bai et al, 2023). In recent years, network pharmacology has been widely utilized in traditional Chinese medicine research to identify the treatment mechanisms for gout and its complications (McReynolds et al, 2019; Xu et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy

Xu,

Yu,

Wang

et al. 2024

Biomedical Chromatography

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Resveratrol (Res) has been demonstrated to have beneficial effects on gouty nephropathy (GN). However, the mechanisms of Res on GN remain unclear. This study aimed to investigate the mechanisms of Res on GN. In this study, network pharmacology technology was used to predict the Res targets in the prevention and treatment of GN. Renal metabonomics was used to identify differential metabolites in kidney tissue of GN model rats. Finally, molecular docking technology was used to verify the binding ability of Res to key targets. Metabonomics analysis showed that 24 potentially important metabolites were involved in the prevention and treatment of GN with Res. After exposure to Res, metabolite levels normalized. The network pharmacology analysis showed that 24 key targets were involved in the prevention and treatment of GN disease. According to the metabolite–gene network diagram, we identified two core genes, PTGS1 and PTGS2, and found that both were involved in the arachidonic acid metabolism pathway. Molecular docking further verified the affinity of Res binding to PTGS1 and PTGS2. In conclusion, the mechanism of Res against GN may be the regulation of arachidonic acid metabolism through the regulation of PTGS 1 and PTGS 2.

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Potential anti-gout properties of Wuwei Shexiang pills based on network pharmacology and pharmacological verification

Cited by 7 publications

References 36 publications

Quality assessment and Q‐markers discovery in Citri Sarcodactylis Fructus by integrating serum pharmacochemistry and network pharmacology

Quality assessment and Q‐markers discovery in Citri Sarcodactylis Fructus by integrating serum pharmacochemistry and network pharmacology

TCM and related active compounds in the treatment of gout: the regulation of signaling pathway and urate transporter

Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy

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