Potential and Limitations of Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells in Musculoskeletal Disorders Treatment

Dias, Isabelle Xavier; Cordeiro, Aline; Guimarães, João Antonio Matheus; Silva, Karina Ribeiro

doi:10.3390/biom13091342

Cited by 4 publications

(2 citation statements)

References 84 publications

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“…We focused on the most studied MSC types for musculoskeletal regenerative medicine approaches for both orthobiologics and GMP-compliant cells. In recent years, other MSC types, such as iPSC-derived [44], were proposed, and the analysis presented will need a revision after these new sources are extensively studied. Also, the choice of HKGs analyzed in this study reflects a subset commonly described in the literature.…”

Section: Discussionmentioning

confidence: 99%

Stable Housekeeping Genes in Bone Marrow, Adipose Tissue, and Amniotic Membrane-Derived Mesenchymal Stromal Cells for Orthopedic Regenerative Medicine Approaches

Ragni,

Piccolo,

Papait

et al. 2024

IJMS

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The therapeutic effect of mesenchymal stromal cells (MSCs) has been described for a variety of disorders, including those affecting musculoskeletal tissues. In this context, the literature reports several data about the regenerative effectiveness of MSCs derived from bone marrow, adipose tissue, and an amniotic membrane (BMSCs, ASCs, and hAMSCs, respectively), either when expanded or when acting as clinical-grade biologic pillars of products used at the point of care. To date, there is no evidence about the superiority of one source over the others from a clinical perspective. Therefore, a reliable characterization of the tissue-specific MSC types is mandatory to identify the most effective treatment, especially when tailored to the target disease. Because molecular characterization is a crucial parameter for cell definition, the need for reliable normalizers as housekeeping genes (HKGs) is essential. In this report, the stability levels of five commonly used HKGs (ACTB, EF1A, GAPDH, RPLP0, and TBP) were sifted into BMSCs, ASCs, and hAMSCs. Adult and fetal/neonatal MSCs showed opposite HKG stability rankings. Moreover, by analyzing MSC types side-by-side, comparison-specific HKGs emerged. The effect of less performant HKG normalization was also demonstrated in genes coding for factors potentially involved in and predicting MSC therapeutic activity for osteoarthritis as a model musculoskeletal disorder, where the choice of the most appropriate normalizer had a higher impact on the donors rather than cell populations when compared side-by-side. In conclusion, this work confirms HKG source-specificity for MSCs and suggests the need for cell-type specific normalizers for cell source or condition-tailored gene expression studies.

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Section: Discussionmentioning

confidence: 99%

Stable Housekeeping Genes in Bone Marrow, Adipose Tissue, and Amniotic Membrane-Derived Mesenchymal Stromal Cells for Orthopedic Regenerative Medicine Approaches

Ragni,

Piccolo,

Papait

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…The causes of MSC heterogeneity have been extensively described in the preceding reviews, and we will not delve into them further. Pluripotent stem cell derived MSC, which can avoid the heterogeneity induced by the aforementioned factors, is also an important category of MSCs [ 13 , 14 ]. However, we will focus exclusively on MSCs derived from somatic cells under natural conditions in this review.…”

Section: Introductionmentioning

confidence: 99%

Unveiling heterogeneity in MSCs: exploring marker-based strategies for defining MSC subpopulations

Chen,

Liang,

2024

J Transl Med

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Mesenchymal stem/stromal cells (MSCs) represent a heterogeneous cell population distributed throughout various tissues, demonstrating remarkable adaptability to microenvironmental cues and holding immense promise for disease treatment. However, the inherent diversity within MSCs often leads to variability in therapeutic outcomes, posing challenges for clinical applications. To address this heterogeneity, purification of MSC subpopulations through marker-based isolation has emerged as a promising approach to ensure consistent therapeutic efficacy. In this review, we discussed the reported markers of MSCs, encompassing those developed through candidate marker strategies and high-throughput approaches, with the aim of explore viable strategies for addressing the heterogeneity of MSCs and illuminate prospective research directions in this field.

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Adipose‐derived mesenchymal stem cell‐incorporated PLLA porous microspheres for cartilage regeneration

Gao,

Yuan,

Wang

et al. 2024

Anim Models and Exp Med

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BackgroundIn facial plastic surgery, patients with nasal deformity are often treated by rib cartilage transplantation. In recent years, cartilage tissue engineering has developed as an alternative to complex surgery for patients with minor nasal defects via injection of nasal filler material. In this study, we prepared an injectable nasal filler material containing poly‐L‐lactic acid (PLLA) porous microspheres (PMs), hyaluronic acid (HA) and adipose‐derived mesenchymal stem cells (ADMSCs).MethodsWe seeded ADMSCs into as‐prepared PLLA PMs using our newly invented centrifugation perfusion technique. Then, HA was mixed with ADMSC‐incorporated PLLA PMs to form a hydrophilic and injectable cell delivery system (ADMSC‐incorporated PMH).ResultsWe evaluated the biocompatibility of PMH in vitro and in vivo. PMH has good injectability and provides a favorable environment for the proliferation and chondrogenic differentiation of ADMSCs. In vivo experiments, we observed that PMH has good biocompatibility and cartilage regeneration ability.ConclusionIn this study, a injectable cell delivery system was successfully constructed. We believe that PMH has potential application in cartilage tissue engineering, especially in nasal cartilage regeneration.

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Potential and Limitations of Induced Pluripotent Stem Cells-Derived Mesenchymal Stem Cells in Musculoskeletal Disorders Treatment

Cited by 4 publications

References 84 publications

Stable Housekeeping Genes in Bone Marrow, Adipose Tissue, and Amniotic Membrane-Derived Mesenchymal Stromal Cells for Orthopedic Regenerative Medicine Approaches

Stable Housekeeping Genes in Bone Marrow, Adipose Tissue, and Amniotic Membrane-Derived Mesenchymal Stromal Cells for Orthopedic Regenerative Medicine Approaches

Unveiling heterogeneity in MSCs: exploring marker-based strategies for defining MSC subpopulations

Adipose‐derived mesenchymal stem cell‐incorporated PLLA porous microspheres for cartilage regeneration

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