Potential Allergenicity Response to Moringa oleifera Leaf Proteins in BALB/c Mice

Zhang, Jie; Li, Xuan; Wang, Zhongliang; Zhang, Hua; Gao, Jinyan; Wu, Yong; Meng, Xuanyi; Zhong, You-Bao; Chen, Hongbing

doi:10.3390/nu14214700

Cited by 6 publications

(3 citation statements)

References 68 publications

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“…However, it should be noted that some individuals may be allergic to M. oleifera leaves. Previous studies have investigated M. oleifera leaf allergens [ 6 , 7 , 8 ], but the mechanism through which Mo-Pr or M. oleifera leaf allergen causes allergies as well as its effect on DCs remain unclear. Food allergy is a significant public health issue worldwide.…”

Section: Discussionmentioning

confidence: 99%

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The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

Xi,

Li,

Liu

et al. 2023

Nutrients

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Moringa oleifera leaves are an inexpensive substitute for staple foods. Despite limited data, Moringa oleifera leaf protein (Mo-Pr) may be allergenic in BALB/c mice. In mouse models and allergic patients, dendritic cells (DCs) may be involved in food allergy. In addition, some allergens, including food allergens, can directly activate DCs and induce Th2 polarization. We investigated whether Mo-Pr can modulate the functional profile of murine bone marrow-derived dendritic cells (BMDCs) in vitro. BMDCs were obtained from mouse bone marrow cultured with granulocyte–macrophage colony-stimulating factor (GM-CSF) for 7 days and then treated with lipopolysaccharide (LPS) or Mo-Pr. BMDC phenotypes were evaluated via flow cytometry, cytokine production was assessed using ELISA, the expression of key genes was studied using qRT-PCR, the effects on T-cell differentiation were investigated using mixed lymphocyte reaction (MLR), and transcriptional changes in BMDCs were investigated using RNA-Seq. Mo-Pr-specific IgE was investigated in recipient serum after BMDC transfer. Mo-Pr treatment significantly induced BMDC maturation, increased the expression of CD80/86 and MHC II, resulted in the production of IL-12 and TNF-α, and induced T-cell differentiation. Mo-Pr treatment stimulated BMDCs’ expression of the Th2 promoters OX40L and TIM-4, induced the production of the Th2-type chemokines CCL22 and CCL17, and decreased the Th1/Th2 ratio in vitro. Healthy recipients of Mo-Pr-treated BMDCs produced Mo-Pr-specific IgE.

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Section: Discussionmentioning

confidence: 99%

“…There have been several studies on M. oleifera leaf allergens. Jie Zhang et al successfully sensitized BALB/c mice to M. oleifera leaf protein through oral administration [ 6 ]. Giovanni D’Auria et al identified potential M. oleifera leaf allergens, including morintides and nsLTP, through proteomic analysis [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

Xi,

Li,

Liu

et al. 2023

Nutrients

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“…M. oleiferaleaves are the leafy part of the same plant and are an economic substitute for staple foods. A potential allergenic response to Moringa oleifera leaf proteins (Mo-Pr) has been reported to cause oral allergy in BALB/c mice [11].…”

Section: Introductionmentioning

confidence: 99%

The potential allergens in Moringa oleifera leaf proteins functionally activate murine bone marrow-derived dendritic cells and induce their differentiation toward a Th2-polarizing phenotype

Liu

et al. 2023

Preprint

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BACKGROUNDMoringa oleifera leaves are an inexpensive substitute for staple foods. Despite limited data, Moringa oleifera leaf protein (Mo-Pr) may be allergenic in BALB/c mice. In mouse models and allergic patients, dendritic cells (DCs) may be involved in food allergy. In addition, some allergens, including food allergens, can directly activate DCs and induce Th2 polarization. We investigated whether Mo-Pr can modulate the functional profile of murine BMDCs in vitro. MATERIALS AND METHODS BMDCs were obtained from mouse bone marrow cultured with GM-CSF for 7 days and then treated with LPS or Mo-Pr. BMDC phenotypes were evaluated by flow cytometry, cytokine production by ELISA, expression of key genes and receptors by flow cytometry or qRT-PCR, effects on T-cell differentiation by MLR, Mo-PR-specific IgE in recipient serum after BMDC transfer, and transcriptional changes in BMDCs by RNA-Seq. RESULTS Mo-Pr treatment significantly induced BMDC maturation, increased expression of surface costimulatory molecules CD80/86 and MHCⅡ, production of IL-12 and TNF-α, and induction of T-cell differentiation. Mo-Pr treatment stimulated BMDC expression of the Th2 promoters OX40L and TIM-4, induced production of the Th2-type chemokines CCL22 and CCL17, and decreased the Th1/Th2 ratio in vitro. Healthy recipients of Mo-Pr-treated BMDCs produced Mo-Pr-specific IgE. CONCLUSIONS Mo-Pr functionally activates murine BMDCs and induces differentiation toward a Th2-polarizing phenotype. Mo-Pr can bind to the C-type lectin receptor DC-SIGN to activate the MAPK pathway and polarize BMDCs toward a Th2-polarizing phenotype.

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Environmental, industrial, and health benefits of Moringa oleifera

Mahaveerchand,

Abdul Salam

2024

Phytochem Rev

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The rise of air, water, and soil pollution poses a significant threat to global health, leading to widespread disease and premature mortality. Soil health is vital, ensuring the production of safe food, but it is compromised by pollutants such as heavy metals, pesticides, plastics, and excessive fertilization, resulting in the depletion of beneficial microorganisms and subsequently groundwater contamination. Water bodies are polluted due to contamination from industrial effluents, domestic wastewater, agricultural runoff, and oil spillage, further intensifying environmental pollution. On the other hand, atmospheric pollution, characterized by high emissions of gases, volatile compounds, greenhouse gases, not only impacts the climate but also poses serious risks to human health, leading to respiratory diseases, cardiovascular issues, and increased cancer risks. Thus, the strategic utilization of traditional plants emerges as a potent tool for environmental restoration and improving human health. The plants possess natural filtering capabilities, absorbing pollutants from air, soil, and water, thus mitigating their adverse effects. Through phytoremediation, plants can be actively used to extract and remove contaminants, contributing to detoxification and improving water and soil quality. Additionally, plants offer various health benefits. Moringa oleifera or the drumstick plant belonging to the Moringaceae family is one such indigenous plant with wide applications, that can be grown in extreme arid conditions. Since ancient times, this plant has been used for treating skin infections, anaemia, and blood impurities. This plant thrives in diverse climates addressing over 300 different aliments. Rich in phytochemicals and bioactive compounds, M. oleifera serve as a superfood, offering high nutritional values and exhibiting potential for drug development with fewer side effects. Extensive research has elucidated the diverse properties and applications of M. oleifera, however, in-depth research is needed to identify bioactive molecules, phytochemicals, and protein compounds involved, which will aid in understanding of the mechanisms of action of the plant's diverse functions. Although studies have reported several of individual M. oleifera attributes, there is no comprehensive study available addressing its diverse applications. This review covers the findings of past three decades and provides a detailed outline of M. oleifera plant and its various parts, its applications in environmental, industrial, food and health aspects documented to date.

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Potential Allergenicity Response to Moringa oleifera Leaf Proteins in BALB/c Mice

Cited by 6 publications

References 68 publications

The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype

The potential allergens in Moringa oleifera leaf proteins functionally activate murine bone marrow-derived dendritic cells and induce their differentiation toward a Th2-polarizing phenotype

Environmental, industrial, and health benefits of Moringa oleifera

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