Potential activity of silver/hydroxyapatite nano composite against damaged liver consequence to irradiation

Abstract

A silver/hydroxyapatite nanocomposite (nAg/HAp) has been formulated by a chemical route and characterized by X-ray analysis spectroscopy, Scanning Electron Microscope, Transmission Electron Microscope. The current study deals with assessing the efficiency of nAg/ HAp against whole body γ-irradiation induced hepatic damage. Twenty-four male rats were assorted into three groups; one group was left as control while the rest two groups were subjected to 8 Gy single dose γ-ray and then treated by an intravenous injection (i.v.) with saline to one group and nAg/HAp solution to the other one. DNA damage and biochemical analyses for hepatic tissue and serum including liver function enzymes, antioxidant enzymes and inflammatory markers were evaluated. Histopathological examination was performed to verify the reached biochemical analyses outcomes. The expermintal results depicted that i.v. nAg/HAp repaired the damage DNA induced by irradiation and this improvement confirmed by decrease in the concentration of 8-hydroxy-deoxyguanosine (8-OHDG) and caspase-3 as well as elevation of the antioxidant enzymes in the hepatic tissue. On the other hand, nAg/HAp significantly recovered serum liver enzymes (AST, ALT, and LDH) total bilirubin (TB) and albumin as well as decreased the levels of MCP-1, IL-6, IL-10, TNF-α and MMP-9. Histopathological examinations exhibited the ability of nAg/HAp in overcoming the damage consequent to irradiation and recovered the hepatic cellular structure for nAg/HAp group with minor change represented by diffusion of some inflammatory cells in between hepatocytes. It can be concluded that nAg/HAp offers a promising treatment against hepatocellular injury induced by γ-irradiation.