Potential activity of recombinant laccase for biodegradation of ampicillin

Nuryana, Isa; Dewi, Kartika Sari; Andriani, Ade; Laksmi, Fina Amreta

doi:10.1088/1755-1315/1201/1/012071

Cited by 3 publications

References 24 publications

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“In Silico” prediction of antibiotics biodegradation by Ganoderma lucidum GILCC 1 laccase

Mora-Gamboa,

Ardila-Leal,

Galindo

et al. 2024

Discov Appl Sci

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Antibiotics present a pressing environmental challenge as emerging pollutants due to their persistence and role in promoting antibiotic-resistant bacteria. To model the utilization of Ganoderma lucidum GlLCC1 laccase in degrading antibiotics, a 3D homology model of GILCC1, based on Lentinus tigrinus mushroom laccase, was utilized. Five broad-spectrum WHO-designated antibiotics with molecular weights between 100 and 500 Da were selected. Molecular dynamics simulations were conducted at pH 3.0 and 7.0 to evaluate the interactions between GILCC1 and antibiotics in a TIP3P water box, with system behaviour assessed at 300 °K using an NPT assembly. ABTS (2,2ʹ-Azino-bis (3-ethylbenzthiazoline-6-sulfonic Acid)) served as the comparison molecule. The binding free energy indicated a strong affinity between 3D GILCC1 and various ligands. At pH 3.0, GILCC1 exhibited significant Gibbs free energy (ΔG), indicating a high affinity for Levofloxacin (LVX; −8.2 kcal mol−1), Sulfisoxazole (SFX; −7.8 kcal mol−1), Cefuroxime (CXM; −7.5 kcal mol−1), Cephradine (CFD; −7. 5 kcal mol−1), ABTS (−7.6 kcal mol−1), and Tetracycline (TE; −7.5 kcal mol−1), attributed to pocket topology and interactions such as hydrogen bonds and van der Waals forces. Electron transfer in GILCC1 involved a chain of residues, including His395 and Phe239. Although the affinity decreased at pH 7.0, the potential of GILCC1 to degrade antibiotics remained plausible. This study accurately predicted the behaviour of the laccase-antibiotic system, providing atomic-level insights into molecular interactions and emphasizing the importance of experimental assays and assessments of antibiotic degradation in wastewater, considering various chemical compounds. The use of ABTS as a mediator was suggested to enhance molecule affinity. Graphical abstract

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“In Silico” prediction of antibiotics biodegradation by Ganoderma lucidum GILCC 1 laccase

Mora-Gamboa,

Ardila-Leal,

Galindo

et al. 2024

Discov Appl Sci

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Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization

Ortolan,

Contato,

Aranha

et al. 2024

Reactions

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Trametes hirsuta GMA-01 was cultivated in a culture medium supplemented with orange waste, starch, wheat bran, yeast extract, and salts. The fungus produced several holoenzymes, but the laccase levels were surprisingly high. Given the highlighted applicability of laccases in various biotechnological areas with minimal environmental impact, we provided a strategy to increase its production using response surface methodology. The immobilization of laccase into ionic supports (CM-cellulose, DEAE-agarose, DEAE-cellulose, DEAE-Sephacel, MANAE-agarose, MANAE-cellulose, and PEI-agarose) was found to be efficient and recuperative, showcasing the technical prowess of research. The crude extract laccase (CE) and CM-cellulose-immobilized crude extract (ICE) showed optimum activity in acidic conditions (pH 3.0) and at 70 °C for the CE and 60 °C for the ICE. The ICE significantly increased thermostability at 60 °C for the crude extract, which retained 21.6% residual activity after 240 min. The CE and ICE were successfully applied to sugarcane bagasse hydrolysis, showing 13.83 ± 0.02 µmol mL−1 reducing sugars after 48 h. Furthermore, the CE was tested for dye decolorization, achieving 96.6%, 71.9%, and 70.8% decolorization for bromocresol green, bromophenol blue, and orcein, respectively (0.05% (w/v) concentration). The properties and versatility of T. hirsuta GMA-01 laccase in different biotechnological purposes are interesting and notable, opening several potential applications and providing valuable insights into the future of biotechnological development.

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Leveraging the Pharmaceutical Area Through Multidisciplinary Synergy

Razak,

Diba,

Xin

et al. 2024

Advances in Higher Education and Professional Development

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The environmental implications of pharmaceutical industry drawn the attention of environmental scientists, prompting multidisciplinary collaborations toward sustainable solutions. Driven by the urgent need to combat the environmental persistence of pharmaceuticals, scholars across diverse disciplines including pharmaceutical sciences, biotechnology and chemical engineering are collaborating to develop effective solutions. Biotechnology uses microbes' natural breakdown power, while chemical engineering creates ideal conditions for efficient drug removal. In this work, a transdisciplinary approach was employed to study a model system comprising ibuprofen and a laccase enzyme. By empowering researchers with a comprehensive understanding of multidisciplinary approaches, the project seeks to optimize reaction conditions for maximized drug degradation efficiency, contributing to environmental protection and cleaner water sources.

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Potential activity of recombinant laccase for biodegradation of ampicillin

Cited by 3 publications

References 24 publications

“In Silico” prediction of antibiotics biodegradation by Ganoderma lucidum GILCC 1 laccase

“In Silico” prediction of antibiotics biodegradation by Ganoderma lucidum GILCC 1 laccase

Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization

Leveraging the Pharmaceutical Area Through Multidisciplinary Synergy

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