Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism

Mohamed, Elsnoussi Ali Hussin; Siddiqui, Jamshed; Ang, Lee Fung; Sadikun, Amirin; Chan, Sue Hay; Tan, Soo Choon; Asmawi, Mohd Zaini; Yam, Mun Fei

doi:10.1186/1472-6882-12-176

Cited by 161 publications

(127 citation statements)

References 26 publications

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“…Indonesia menempati posisi ketujuh negara dengan penderita diabetes terbanyak di dunia (IDF, 2013). Kumis kucing merupakan tanaman obat tradisional Indonesia yang digunakan untuk mengobati diabetes dengan cara menghambat aktivitas enzim pencernaan salah satunya enzim α-glukosidase (Mohamed et al, 2011;Mohamed et al, 2012). Selain anti hiperglikemik, tanaman kumis kucing kaya akan senyawa antioksidan (Akowuah et al, 2005) sehingga kumis kucing berpotensi pula untuk menurunkan risiko komplikasi diabetes akibat stres oksidatif (Baynes dan Thorpe, 1999).…”

Section: Pendahuluanunclassified

“…Ekstrak etanol daun kumis kucing dilaporkan memiliki aktivitas penghambatan terhadap enzim α -glukosidase dengan IC 50 sebesar 4,63 mg/mL (Mohamed et al, 2012) lebih besar dibandingkan dengan nilai IC 50 yang diperoleh dari hasil penelitian yaitu sebesar sebesar 465,83 µg/mL. Perbedaan asal geografis tempat tumbuh, jenis pelarut dan bagian tanaman yang yang digunakan mem-pengaruhi kandungan bioaktif sehingga dapat menyebabkan perbedaan aktifitas biologis dari tanaman kumis kucing (Rafi et al, 2015;Akowuah et al, 2005).…”

Section: Hasil Dan Pembahasan Aktivitas Penghambatan α-Glukosidase Ekunclassified

“…Sinensitin merupakan senyawa metoksi flavonoid larut kloroform dengan aktivitas anti α-glukosidase kuat (IC 50 0,66 mg/mL) (Mohamed et al, 2012). Aktivitas penghambatan α-glukosidase oleh fraksi butanol kumis kucing belum pernah dilaporkan akan tetapi senyawa glikosida dan flavonoid dengan aktivitas anti α-glukosidase kuat diisolasi dari fraksi butanol herbal Gynostemma pentaphyllum (Yang et al, 2013).…”

Section: Hasil Dan Pembahasan Aktivitas Penghambatan α-Glukosidase Ekunclassified

“…Nilai VIP≥ ,5 dianggap relevan sedangkan nilai VIP>1 sangat relevan (GalindoPrieto et al, 2014). Sinensitin dengan gugus fungsi dari grup metoksi, asam karboksilat, cincin aromatik dan heteroaromatik, metil, hidroksi fenolik, C-O, dan ujung metilena merupakan salah satu senyawa yang diketahui memiliki aktivitas penghambatan terhadap α-glukosidase (Hossain dan Rahman, 2015;Mohamed et al, 2012). Hasil berbeda dikemukakan oleh Damsud et al (2014) yang mengisolasi 4 flavonoid yaitu sinensetin, salvigenin, tetrametilskutellarein dan 3,7,4'-tri-O-metilkaempferol.…”

Section: Penanda Senyawa Penghambat Aktivitas Enzim α-Glukosidaseunclassified

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SENYAWA INHIBITOR Α-Glukosidase DAN ANTIOKSIDAN DARI KUMIS KUCING DENGAN PENDEKATAN METABOLOMIK BERBASIS FTIR

Juliani

Yuliana

Budijanto

et al. 2016

jtip

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Plant is well known as an excellent source for b ioactive compounds. Metab olomics was reported as a potential tool to accelerate plant acitive compounds identification. In this research, FTIR-b ased metab olomics method was used to identify active compounds with α-glucosidase inhib itory and antioxidant activity in aerial parts of Orthosiphon stamineus (OS OPLS). Based on the VIP (variab le influence on projection), the coefficient value of the respective OPLS models, and IR datab ase of compounds previously identified in OS, it was suggested that methoxy flavonoid (sinensitin and 5,6,7,3'-tetramethoxy-4'-hydroxy-8-C-preny-lflavone), diterpenes (orthosiphols, orthoarisins, neoorthosiphols, staminols, and stam inolactones) and triterpenes (ursolic acid, oleanolic acid, b etulinic acid, hydroxyb etulinic acid, maslinic acid, α-amyrin and β-amyrin) were identified as responsib le compounds for the α-glucosidase inhibitory activity. Meanwhile phenolic (rosmarinic acid ), methoxy flavonoid (eupatorin, sinensetin, 5-hydroxy-6,7,3',4'-tetramethoxyflavone, salvigenin, 6-hydroxy-5,7,3'-trimethoxyflavone and 5,6,7,3'-tetramethoxy-4'-hydroxy-8-C-prenylflavone), diterpenes (orthosiphols, orthoarisins, neoorthosiphols, staminols, and staminolactones) and triterpenes (ursolic acid, oleanolic acid, b etulinic acid, hydroxyb etulinic acid, maslinic acid, α-amyrin and β-amyrin) were identified as responsib le compounds for the antioxidant activity. Keywords: α-glucosidase inhib itors, antioxidants, metab olomics, Orthosiphon stamineus ABSTRAK1Tumbuhan merupakan salah satu sumber senyawa aktif yang potensial. Pendekatan metabolomik dilaporkan sebagai salah satu metode alternatif yang dapat mempercepat proses identifikasi komponen aktif dari tumbuhan. Pada penelitian ini, metode metabolomik berbasis FTIR digunakan untuk meng-identifikasi senyawa aktif dengan aktivitas penghambatan terhadap enzim α-glukosidase dan antioksidan pada ekstrak dan fraksi tanaman kumis kucing (OS). Profil kimia ekstrak metanolik dan fraksi heksana, kloroform, butanol, dan air OS diukur dengan FTIR. Ekstrak dan fraksi OS menunjukkan aktivitas penghambatan terhadap enzim α-glukosidase dengan nilai IC50 berkisar 154,07±30,60-465,83±85,34 µg/mL dan aktivitas antioksidan dengan nilai IC50 berkisar 7,41±0,02-19,35±0,09 µg/mL. Fraksi butanol adalah fraksi dengan aktivitas penghambatan α-glukosidase tertinggi dan aktivitas antioksidan menengah dengan nilai IC 50 berturut-turut turut 154,07±30,60 µg/mL dan 10,84±0,54 µg/mL. Korelasi antara data aktivitas biologis dan komposisi kimia dianalisis dengan orthogonal projections to latent structures (OPLS). Berdasarkan nilai VIP (variab le influence on projection), nilai koefisien dari model OPLS, dan pangkalan data IR senyawa yang telah teridentifikasi pada OS. Metoksi flavonoid (sinensitin dan 5,6,7,3'-tetrametoksi-4'-hidroksi-8-C-prenilflavon), diterpena (ortosifol, ortoarisin, neoortosifol, staminol, dan staminolakton), triterpena (asam ursolat, asam oleanolat, asam betulinat, asam hidroksibetulinat, ...

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Section: Pendahuluanunclassified

Section: Hasil Dan Pembahasan Aktivitas Penghambatan α-Glukosidase Ekunclassified

Section: Penanda Senyawa Penghambat Aktivitas Enzim α-Glukosidaseunclassified

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SENYAWA INHIBITOR Α-Glukosidase DAN ANTIOKSIDAN DARI KUMIS KUCING DENGAN PENDEKATAN METABOLOMIK BERBASIS FTIR

Juliani

Yuliana

Budijanto

et al. 2016

jtip

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“…The O. stamineus extract used in this study was more in a crude form, which may explain its low antibacterial activity. Previous studies which reported the protective effects of O. stamineus employed methanolic (Chun-Hoong et al, 2010;Malahubban et al, 2013) and ethanolic extracts (Alshawsh et al, 2012;Mohamed et al, 2012).…”

Section: Journal Of Agricultural Sciencementioning

confidence: 99%

In vitro Antibacterial Activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis

Abidin¹,

Abdul-Wahab²,

Ghazi-Ahmad³

et al. 2017

JAS

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This study evaluates the antibacterial effects of Zingiber officinale essential oil and Orthosiphon stamineus water extract against Enterococcus faecalis. The herbs were prepared in various concentrations to determine their minimum inhibitory concentrations (MIC) and growth inhibitory effect. Anti-adhesion activities of the herbs were determined by co-incubation with E. faecalis cultures for 6 and 24 h. Biofilm disruption activities were determined by adding the studied herbs into preformed E. faecalis biofilm. The effects on the morphology of E. faecalis grown as biofilm were studied using scanning electron microscopy (SEM). The MICs of ginger oil and O. stamineus extract were 0.31 and 25 mg/mL, respectively. Between the tested herbs, ginger exhibited greater inhibitory effects on the growth of E. faecalis grown in suspension mode. Both herbs generally showed anti-adhesion activities in inverse concentration-dependent manner. No significant biofilm disruption activities by both herbs were observed. SEM analyses showed E. faecalis cell surface changes in the treated biofilm. The studied herbs may have compromised the integrity of the bacterial cell membrane. These findings suggest that the studied herbs may have better antibacterial activities against E. faecalis in suspension mode compared to biofilm mode, with ginger oil showed greater antibacterial activity compared to O. stamineus extract.

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Hypoglycaemic Effects of Plants Food Constituents via Inhibition of Carbohydrate‐Hydrolysing Enzymes: From Chemistry to Future Applications

Loizzo

Bonesi

Nabavi

et al. 2017

Natural Products Targeting Clinically Relevant Enzymes

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Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism

Cited by 161 publications

References 26 publications

SENYAWA INHIBITOR Α-Glukosidase DAN ANTIOKSIDAN DARI KUMIS KUCING DENGAN PENDEKATAN METABOLOMIK BERBASIS FTIR

SENYAWA INHIBITOR Α-Glukosidase DAN ANTIOKSIDAN DARI KUMIS KUCING DENGAN PENDEKATAN METABOLOMIK BERBASIS FTIR

In vitro Antibacterial Activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis

Hypoglycaemic Effects of Plants Food Constituents via Inhibition of Carbohydrate‐Hydrolysing Enzymes: From Chemistry to Future Applications

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