Potent insect gut binding lectin from Sclerotium rolfsii impart resistance to sucking and chewing type insects in cotton

Vanti, Gulamnabi L.; Katageri, I. S.; Hiremathada, Vamadevaiah; Swamy, Bale M.

doi:10.1016/j.jbiotec.2018.04.018

Cited by 17 publications

(8 citation statements)

References 37 publications

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“…Interestingly, SRL-expressing plants showed also resistance to sucking and chewing insects S. litura and Myzus persicae [ 43 ]. Accordingly, SRL-transgenic cotton plants showed high resistance to Aphis gossypii (69 % reduction in population) and S. litura (100 % larval mortality) [ 44 ]. These studies suggest that MOA may provide protection against other insect herbivores.…”

Section: Discussionmentioning

confidence: 99%

Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect

et al. 2021

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Background Plant-parasitic nematodes and herbivorous insects have a significant negative impact on global crop production. A successful approach to protect crops from these pests is the in planta expression of nematotoxic or entomotoxic proteins such as crystal proteins from Bacillus thuringiensis (Bt) or plant lectins. However, the efficacy of this approach is threatened by emergence of resistance in nematode and insect populations to these proteins. To solve this problem, novel nematotoxic and entomotoxic proteins are needed. During the last two decades, several cytoplasmic lectins from mushrooms with nematicidal and insecticidal activity have been characterized. In this study, we tested the potential of Marasmius oreades agglutinin (MOA) to furnish Arabidopsis plants with resistance towards three economically important crop pests: the two plant-parasitic nematodes Heterodera schachtii and Meloidogyne incognita and the herbivorous diamondback moth Plutella xylostella. Results The expression of MOA does not affect plant growth under axenic conditions which is an essential parameter in the engineering of genetically modified crops. The transgenic Arabidopsis lines showed nearly complete resistance to H. schachtii, in that the number of female and male nematodes per cm root was reduced by 86–91 % and 43–93 % compared to WT, respectively. M. incognita proved to be less susceptible to the MOA protein in that 18–25 % and 26–35 % less galls and nematode egg masses, respectively, were observed in the transgenic lines. Larvae of the herbivorous P. xylostella foraging on MOA-expression lines showed a lower relative mass gain (22–38 %) and survival rate (15–24 %) than those feeding on WT plants. Conclusions The results of our in planta experiments reveal a robust nematicidal and insecticidal activity of the fungal lectin MOA against important agricultural pests which may be exploited for crop protection.

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Section: Discussionmentioning

confidence: 99%

Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect

et al. 2021

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“…( Trung et al, 2006 ). The transgenic cotton lines containing insect gut binding lectin demonstrated significant level of resistance to sucking and chewing insects at T 1 generation ( Vanti et al, 2018 ). The lentil lectin (LL) and chickpea protease inhibitor (CPPI) genes transferred to Brassica juncea lines and the resulting transgenic plants showed resistance to sap-sucking pests such as aphids ( Rani et al, 2017b ).…”

Section: Biotechnological Approaches In Insect Pest Managementmentioning

confidence: 99%

Biotechnological Approaches for Host Plant Resistance to Insect Pests

et al. 2022

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Annually, the cost of insect pest control in agriculture crosses billions of dollars around the world. Until recently, broad-spectrum synthetic pesticides were considered as the most effective means of pest control in agriculture. However, over the years, the overreliance on pesticides has caused adverse effects on beneficial insects, human health and the environment, and has led to the development of pesticide resistant insects. There is a critical need for the development of alternative pest management strategies aiming for minimum use of pesticides and conservation of natural enemies for maintaining the ecological balance of the environment. Host plant resistance plays a vital role in integrated pest management but the development of insect-resistant varieties through conventional ways of host plant resistance takes time, and is challenging as it involves many quantitative traits positioned at various loci. Biotechnological approaches such as gene editing, gene transformation, marker-assisted selection etc. in this direction have recently opened up a new era of insect control options. These could contribute towards about exploring a much wider array of novel insecticidal genes that would otherwise be beyond the scope of conventional breeding. Biotechnological interventions can alter the gene expression level and pattern as well as the development of transgenic varieties with insecticidal genes and can improve pest management by providing access to novel molecules. This review will discuss the emerging biotechnological tools available to develop insect-resistant engineered crop genotypes with a better ability to resist the attack of insect pests.

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“…The successful transfer of Bt and other toxin genes into crops, and their effect on enhanced pest resistance has been reported in rice, maize, and cotton [ 20 , 21 , 22 ]. However, the use of insecticidal genes is not without its limitations: target pests can evolve resistance to these genetically-engineered defenses [ 23 , 24 , 25 ]. To minimize the problem of insect adaptation to current insecticidal genes, modifying existing toxin genes or pyramiding various resistance genes against target organisms have found some success.…”

Section: Introductionmentioning

confidence: 99%

Expression of Modified Snowdrop Lectin (Galanthus nivalis Agglutinin) Protein Confers Aphids and Plutella xylostella Resistance in Arabidopsis and Cotton

Jia

Xue

et al. 2022

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Cotton is a major fiber crop in the world that can be severely infested by pests in agricultural fields. Identifying new insect-resistance genes and increasing the expression of known insect-resistance genes are imperative in cultivated cotton. Galanthus nivalis agglutinin (GNA), a lectin that is toxic to both chewing and sucking pests, is mainly expressed in monocotyledons. It is necessary to improve the expression of the GNA protein and to test whether the lectin confers insect resistance to dicotyledons plants. We report a modified GNA gene (ASGNA) via codon optimization, its insertion into Arabidopsis thaliana, and transient expression in cotton to test its efficacy as an insect-resistance gene against cotton aphids and Plutella xylostella. The amount of ASGNA in transgenic plants reached approximately 6.5 μg/g of fresh weight. A feeding bioassay showed that the survival rate of aphids feeding on the leaves of ASGNA transgenic plants was lower than those of aphids feeding on the leaves of non-optimized GNA (NOGNA) transgenic plants and wild-type plants. Meanwhile, the fertility rate was 36% when fed on the ASGNA transgenic plants, while the fertility was 70% and 95% in NOGNA transgenic plants and wild-type plants. Correspondingly, the highest mortality of 55% was found in ASGNA transgenic lines, while only 35% and 20% mortality was observed in NOGNA transgenic plants and wild-type plants, respectively. Similar results were recorded for aphids feeding on cotton cotyledons with transient expression of ASGNA. Taken together, the results show that ASGNA exhibited high insecticidal activity towards sap-sucking insects and thus is a promising candidate gene for improving insect resistance in cotton and other dicotyledonous plants.

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Potent insect gut binding lectin from Sclerotium rolfsii impart resistance to sucking and chewing type insects in cotton

Cited by 17 publications

References 37 publications

Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect

Marasmius oreades agglutinin enhances resistance of Arabidopsis against plant-parasitic nematodes and a herbivorous insect

Biotechnological Approaches for Host Plant Resistance to Insect Pests

Expression of Modified Snowdrop Lectin (Galanthus nivalis Agglutinin) Protein Confers Aphids and Plutella xylostella Resistance in Arabidopsis and Cotton

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