Potent immune responses induced by a <i>Salmonella</i> ghost delivery system that expresses the recombinant Stx2eB, FedF, and FedA proteins of the <i>Escherichia coli</i>-producing F18 and Shiga toxin in a murine model and evaluation of its protective effect as a porcine vaccine candidate

Won, Gayeon; Hwa, Lee John

doi:10.1080/01652176.2017.1308040

Cited by 5 publications

(1 citation statement)

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“…While ETEC neonatal diarrhea can be effectively controlled by vaccination of pregnant sows, passive protection is quickly lost after weaning ( Melkebeek et al, 2013 ; Matías et al, 2017 ). Currently, few vaccines are commercially available for PWD, and few provide protection against different E. coli pathotypes ( Nadeau et al, 2017 ; Won and John Hwa, 2017 ). In the present study, we performed a wide epidemiological study on a collection of E. coli isolates recovered from 464 faecal samples of enteric colibacillosis in pig production farms of Spain over a 10-year period to know the E. coli traits (pathotypes) presently implicated in swine diarrheas, and how the use of antibiotics (including colistin) could have affected the selection and emergence of resistant strains.…”

Section: Discussionmentioning

confidence: 99%

Swine Enteric Colibacillosis in Spain: Pathogenic Potential of mcr-1 ST10 and ST131 E. coli Isolates

et al. 2018

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This is a wide epidemiological study of 499 E. coli isolates recovered from 179 outbreaks of enteric colibacillosis from pig production farms in Spain during a period of 10 years. Most samples were of diarrheagenic cases occurred during the post-wean period (PWD) which showed to be significantly associated with ETEC (67%) followed by aEPEC (21.7%). On the contrary, aEPEC was more prevalent (60.3%) among diarrheas of suckling piglets, followed by ETEC (38.8%). STEC/ETEC or STEC were recovered in 11.3 and 0.9% of PWD and neonatal diarrhea, respectively. Detection of the F4 colonization factor was not significantly different between isolates recovered from neonatal pigs and those recovered post wean (40.5 versus 27.7%) while F18 was only present among PWD isolates (51.5% of ETEC, STEC, and STEC/ETEC isolates). We also found a high prevalence of resistance to colistin related to the presence of the mcr-1 gene (25.6% of the diarreagenic isolates). The characterization of 65 representative mcr-1 isolates showed that all were phenotypically resistant to colistin (>2 μg/ml), and most (61 of 65) multidrug-resistant (MDR). Six ETEC and one STEC mcr-1 isolates were also carriers of ESBL genes. In addition, other seven mcr-1 isolates harbored mcr-4 (three ETEC) and mcr-5 (two ETEC and two aEPEC) genes. In the phylogenetic analysis of the 65 mcr-1 diarrheagenic isolates we found that more than 50% (38 out of 65) belonged to A-ST10 Cplx and from those, 29 isolates showed the clonotype CH11-24. In this study, we also recovered 18 ST131 isolates including seven mcr-1 carriers. To the best of our knowledge, this would be the first report of ST131 mcr-1 isolation in pigs. Worryingly, the swine mcr-1 ST131 carriers also showed MDR, including to trimethoprim-sulfamethoxazole, tobramycin, gentamicin and ciprofloxacin. In the PFGE-macrorestriction comparison of clinical swine and human ST131, we found high similarities (≥85%) between two pig and two human ST131 isolates of virotype D5. Acquisition of mcr-1 by this specific clone means an increased risk due to its special feature of congregating virulence and resistance traits, together with its spread capability. Here we show a potential zoonotic swine source of ST131.

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