Potent in vivo anticancer activity and stability of liposomes encapsulated with semi-purified Job’s tear (Coix lacryma-jobi Linn.) extracts on human colon adenocarcinoma (HT-29) xenografted mice

Abstract

Potent in vivo anticancer activity and stability of liposomes encapsulated with semi-purified Job's tear (Coix lacryma-jobi Linn.) extracts on human colon adenocarcinoma AbstractThe in vivo anticancer activity and stability of liposomes encapsulated with semi-purified Job's tear (Coix lacryma-jobi Linn.) extracts (S5L), prepared by supercritical carbon dioxide fluid technique, on human colon adenocarcinoma (HT29) xenografted mice were investigated. For the stability and the physicochemical characteristics, S5L showed a high stability of pH, good dispersibility, small particle size and stable zeta potential. Liposomes can protect linoleic acid in the extract comparing with the free S5. S5L kept at 4 C for 3 months showed the highest linoleic acid content of 63.50%, whereas at 45 C, the lowest linoleic acid content of 42.66% was observed. The anticancer activity and toxicity on xenografted mice were observed for 14 days. At the end of the experiment, the relative tumor volume (RTV) in the S5L-treated xenografted mice showed a significant RTV reduction. The high dose of S5 and S5L were potent with the highest inhibition of tumor growth of 48.67 and 54.75%, which was 86.94% and 97.81% of 5-fluorouracil, respectively. The apoptotic activity was shown in xenografted mice treated with S5 at medium and high dose, S5L, 5-fluorouracil and commercial product. All treated xenografted mice showed no toxic signs and symptoms, abnormality of internal organs histopathology and blood chemistry. This study has demonstrated the high physicochemical stability of liposomes encapsulated with semi-purified Job's tear extract and their potent anticancer activity on human colon adenocarcinoma xenografted model with the potential for further development to anticolon cancer drug.

Preparation and Evaluation of Liposomes Co-Loaded with Doxorubicin, Phospholipase D Inhibitor 5-Fluoro-2-Indolyl Deschlorohalopemide (FIPI) and D-Alpha Tocopheryl Acid Succinate (α-TOS) for Anti-Metastasis

“…The carboxyl group of α-TOS located in the lipophilic interfacial region where it increased the membrane surface charge dissociated into negative ion at physiological pH, indicating that the zeta negative potential of the liposomes was further increased compared to that of the Blank liposomes. The enhanced electrostatic repulsion between particles helps to stabilize the liposomes during storage [51] and clinical application.…”

Actional Mechanisms of Active Ingredients in Functional Food Adlay for Human Health

“…The potent anti-proliferative activity against HT-29 cells of liposomes entrapped with adlay with high physicochemical stability could enable it to be developed as a novel anti-cancer drug for humans [ 87 , 88 ]. An EtOAc extract of adlay showed strong anti-tumor activity and was a natural anti-tumor bioactive agent, which produced triolein reached at a rate of 2.536 ± 0.006 mg/g dry weight of mycelium [ 89 ].…”

Adlay, an ancient functional plant with nutritional quality, improves human health

“…Coicis oil contains coixenolide, palmitic acid, stearic acid, octadecenoic acid, myristic acid, and palmitate ( 45 , 46 ). Coix seed volatile oil contains 69 components, the main of which are hexanal, hexanoic acid, 2-ethyl-3-hydroxy-hexylbutrate, γ-non-aldactone, nonanoic acid, octanoic acid, ethylpalmitate, methyllinoleate, vanillin, and ethyl linoleate ( 47 ). At low concentrations, coicis oil has a stimulatory effect on breathing, and on cardiac striated and smooth muscles, which can significantly dilate pulmonary blood vessels and improve blood circulation in the lungs ( 39 , 48 ).…”

“… BCL-2, B-cell Lymphoma 2; PTEN, phosphatase and tensin homolog deleted on chromosome ten; PI3K/AKT, phosphoinositide 3-kinase/protein kinase B; MMPs, matrix metalloproteinase; TIMPs, tissue inhibitor of metalloproteinases; JAK2/STAT3, just another kinase2/signal transducer and activator of transcription 3; NF-κB-MuRF1, nuclear factor of kappa B/muscle ring finger protein1; AMPK-HSL, adenylate activated protein kinase-hormone sensitive lipase ( 13 , 30 , 36 , 45 , 47 , 48 , 51 , 55 , 56 , 59 , 65 , 66 , 69 , 72 ). …”