Potent humanin analogue (HNG) protects human sperm from freeze-thaw-induced damage

Yang, Chao; Li, Xu; Cui, Yingdong; Wu, Bo; Liao, Zhaolin

doi:10.1016/j.cryobiol.2019.04.001

Cited by 17 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since the 1960s, sperm cryopreservation has been introduced as a routine practice in infertility clinics (Najafi et al, ). This technique is being utilised for several indications, including donor insemination programmes, before treatments which my hurt fertility potential such as chemotherapy, radiotherapy, orchiectomy and vasectomy, testicular sperm retrieval in azoospermic cases and epididymal sperm aspiration (obstructive azoospermia), gender reassignment, spinal cord injury and hypogonadotropic hypogonadism (Banihani & Alawneh, ; Pabon & Meseguer, ; Wierckx et al, ; Yang, Xu, Cui, Wu, & Liao, ). Freezing spermatozoon is performed before intracytoplasmic sperm injection (ICSI) to rule out cycle cancellation for failure to collect semen specimen on the day of the in vitro fertilisation/ICSI attempt (Montagut et al, ).…”

Section: Introductionmentioning

confidence: 99%

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

et al. 2020

View full text Add to dashboard Cite

To investigate the impact of antioxidants in sperm parameters and reduction in reactive oxygen species production during the freeze‐thaw process. PubMed, Scopus, Web of Science, Embase and Cochrane central library were systematically searched. Of the 1583 articles, 23 studies were selected for data extraction. Our results show that antioxidants improved sperm progressive motility (standardised mean difference (SMD) = 1; 95% CI: 0.62, 1.38; p < .001) and viability (SMD = 1.20; 95% CI: 0.50, 1.91; p = .001) and reduced sperm DNA fragmentation (SDF) and hydrogen peroxide (H2O2) production, but there was no significant improvement in total sperm motility after thawing. Acetyl‐l‐carnitine/l‐carnitine, melatonin and catalase had a significant positive impact on progressive motility. The role of tempol and melatonin in improving viability was significant compared to other antioxidants. Moreover, a significant reduction in SDF was observed after addition of butylated hydroxytoluene, tempol and vitamin E. However, the prevention of H2O2 production was significant only after the addition of tempol. Our overall results displayed the positive impact of antioxidants on progressive sperm motility, viability and reduction in SDF and H2O2 production, but no significant impact of antioxidants on total sperm motility was seen during the freeze‐thaw process.

show abstract

Section: Introductionmentioning

confidence: 99%

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

et al. 2020

View full text Add to dashboard Cite

show abstract

“…A synthetic derivate/analogue of HN, called HN-S14G (HNG), was designed with amino acid substitution of serine at amino acid residue 14 to glycine ( Hashimoto et al, 2001b ), which demonstrated more potent effects than wild type HN on the reduction of intracellular reactive oxygen species (ROS), preservation of mitochondrial membrane potential and structure with cardioprotective activity in vivo ( Klein et al, 2013 ; Minasyan et al, 2017 ). Consistently, HNG also showed reduced damage to the mitochondrial membrane and DNA integrity, and attenuation of the reaction of oxidative stress and the activity of caspase-3 in sperm ( Yang et al, 2019 ). Pre-treatment using HNG also showed protective effects on epidermal stem cells (ESC) against ultraviolet (UV)-B-induced cytotoxicity ( Wang et al, 2019 ).…”

Section: Synthetic Analogues Of Hnmentioning

confidence: 63%

The Molecular Structure and Role of Humanin in Neural and Skeletal Diseases, and in Tissue Regeneration

Zhu

Bennett

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Humanin (HN) belongs to a member of mitochondrial-derived peptides (MDPs) which are encoded by mitochondrial genes. HN shares sequence homology with thirteen HN-like proteins, named MTRNR2L1 to MTRNR2L13, which encompass 24–28 amino acid residues in length. HN mediates mitochondrial status and cell survival by acting via an intracellular mechanism, or as a secreted factor via extracellular signals. Intracellularly, it binds Bcl2-associated X protein (BAX), Bim and tBid, and IGFBP3 to inhibit caspase activity and cell apoptosis. When released from cells as a secreted peptide, HN interacts with G protein-coupled formyl peptide receptor-like 1 (FPRL1/2) to mediate apoptosis signal-regulating kinase (ASK) and c-Jun N-terminal kinase (JNK) signalling pathways. Additionally, it interacts with CNTFR-α/gp130/WSX-1 trimeric receptors to induce JAK2/STA3 signalling cascades. HN also binds soluble extracellular proteins such as VSTM2L and IGFBP3 to modulate cytoprotection. It is reported that HN plays a role in neuronal disorders such as Alzheimer’s disease, as well as in diabetes mellitus, infertility, and cardiac diseases. Its roles in the skeletal system are emerging, where it appears to be involved with the regulation of osteoclasts, osteoblasts, and chondrocytes. Understanding the molecular structure and role of HN in neural and skeletal diseases is vital to the application of HN in tissue regeneration.

show abstract

“…HN inhibited NMDA-induced oxidative stress and nitric oxide (NO) production in cultured rat cortical neurons [75]. Further, HN protected cells from ischemia and reperfusion induced a significant increase in lactate dehydrogenase (LDH) release, MDA formation in cortical neurons and HN also induced ROS generation and MDA levels in human sperm upon freeze-thaw [76]. All these data collectively suggest that HN has a potential to induce protective response to oxidative and nitro-oxidative stress, lipoperoxidation and the carbonylated protein content in SH-SY5Y cells.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial Peptide Humanin Protects Silver Nanoparticles-Induced Neurotoxicity in Human Neuroblastoma Cancer Cells (SH-SY5Y)

Gurunathan

Jeyaraj

Kang

et al. 2019

IJMS

View full text Add to dashboard Cite

The extensive usage of silver nanoparticles (AgNPs) as medical products such as antimicrobial and anticancer agents has raised concerns about their harmful effects on human beings. AgNPs can potentially induce oxidative stress and apoptosis in cells. However, humanin (HN) is a small secreted peptide that has cytoprotective and neuroprotective cellular effects. The aim of this study was to assess the harmful effects of AgNPs on human neuroblastoma SH-SY5Y cells and also to investigate the protective effect of HN from AgNPs-induced cell death, mitochondrial dysfunctions, DNA damage, and apoptosis. AgNPs were prepared with an average size of 18 nm diameter to study their interaction with SH-SY5Y cells. AgNPs caused a dose-dependent decrease of cell viability and proliferation, induced loss of plasma-membrane integrity, oxidative stress, loss of mitochondrial membrane potential (MMP), and loss of ATP content, amongst other effects. Pretreatment or co-treatment of HN with AgNPs protected cells from several of these AgNPs induced adverse effects. Thus, this study demonstrated for the first time that HN protected neuroblastoma cells against AgNPs-induced neurotoxicity. The mechanisms of the HN-mediated protective effect on neuroblastoma cells may provide further insights for the development of novel therapeutic agents against neurodegenerative diseases.

show abstract

Potent humanin analogue (HNG) protects human sperm from freeze-thaw-induced damage

Cited by 17 publications

References 39 publications

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

The Molecular Structure and Role of Humanin in Neural and Skeletal Diseases, and in Tissue Regeneration

Mitochondrial Peptide Humanin Protects Silver Nanoparticles-Induced Neurotoxicity in Human Neuroblastoma Cancer Cells (SH-SY5Y)

Contact Info

Product

Resources

About