Potent Anticariogenic Activity of Aceriphyllum rossii and Its Components, Aceriphyllic Acid A and 3‐oxoolean‐12‐en‐27‐oic Acid

Abstract

Aceriphyllum rossii Engler (Saxifragaceae) have been used as a nutritious food in Korea. We found that the methanol extract of A. rossii root and its components, aceriphyllic acid A and 3-oxoolean-12-en-27-oic acid, potently inhibited the growth of the key cariogenic bacteria, Streptococcus mutans, with MIC of 2 to 4 microg/mL. They also showed antibacterial activity against other cariogenic bacteria such as S. oralis, S. sobrinus, and S. salivarius with the similar potency. In the time-kill study, aceriphyllic acid A reduced the viable counts of S. mutans by 90% in 1 min at 8 microg/mL, indicating that aceriphyllic acid A had the fast bacteriostatic activity. Severe damages of the cell surface of S. mutans by aceriphyllic acid A were observed by transmission electron microscopy, suggesting with its fast antibacterial activity that its mechanism of action might be membrane disruption. These results suggest that the methanol extract of A. rossii root and its components, aceriphyllic acid A and 3-oxoolean-12-en-27-oic acid, could have the great potential as natural agents for preventing dental caries.

3-Hydroxyolean-12-en-27-oic Acids Inhibit RANKL-Induced Osteoclastogenesis in Vitro and Inflammation-Induced Bone Loss in Vivo

“…A. rossii is a rich source of 12-en-27-oic acid-type triterpenes [ 31 , 32 , 33 ]. These triterpenes have a variety of biological effects including anticomplementary, cytotoxic, anti-bacterial, anti-cancer, anti-inflammatory, and diacylglycerol aceyltransferase-inhibitory activities [ 26 , 32 , 33 , 34 , 35 , 36 , 37 ]. In the current study, we demonstrated that two 3-hydroxyolean-12-en-27-oic acid derivatives, AR-3 and AR-5, suppressed RANKL-induced osteoclast differentiation, actin-ring formation, and resorption pit formation.…”

“…A. rossii is a rich source of 12-en-27-oic acid-type triterpenes [31][32][33]. These triterpenes have a variety of biological effects including anticomplementary, cytotoxic, anti-bacterial, anti-cancer, anti-inflammatory, and diacylglycerol aceyltransferase-inhibitory activities [26,[32][33][34][35][36][37].…”

Anticariogenic Activity of <i>Lagerstroemia speciosa</i> (L.)

“…Both the extract were found to be bacteriostatic while malvidin-3,5-diglucoside, isolated from ethanol extract of flowers was found to be the principal constituent for antibacterial activity. In a study, Zheng et al (2010) observed inhibitory efficacy of methanol extract of Aceriphyllum rossii Engler root and its components aceriphyllic acid A and 3-oxoolean-12-en-27-oic acid against all cariogenic bacteria tested. Aceriphyllic acid A was found to possess faster bacteriostatic activity and the inhibitory action was shown to be membrane disruption leading to killing of bacteria.…”