Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro

Abstract

ABSTRACT:Punica granatum flower and leaf were extracted with hydromethanol 50% and evaluated for antioxidant activity using phosophomolybdenum, DPPH, reducing power, and -OH-scavenging assays; and for antiinflammatory activity using HRBC-MS assay. Both extracts exhibited antioxidant activities indicated by DPPH decolorization and -OH-scavenging and increased Ferric reducing and phosphomolybdenum assays. Also, both extracts showed anti-inflammatory activities indicated by red blood cell membrane stabilization and reduced haemolysis-resultant haemoglobin. All results were concentration-dependent; and the leaf extract was more potent than that of the flower. The -OH-scavenging and red blood cell-membrane stability assays, respectively. Phytochemical analysis revealed presence of tannins and flavonoids with results more obvious in the leaf extract. These active principle contents may account for the recorded antioxidant and anti-inflammatory activities of both extracts. These findings provide evidence for the possible beneficial applications of the Punica granatum leaf and flower extracts in stress-related disease conditions and for maintenance of normal health status and well-being as well.

Determination of Antioxidant Capacity and Gallic Acid Content in Ethanolic Extract of Punica Granatum L. Leaf

“…1 shows the dose-dependent DPPH scavenging activity of EPGL, AA, and GA with an IC 50 value of 136 µg/ml, 30.5 µg/ml, and 38.5 µg/ml. Our results (IC 50 =136 µg/ml) were similar to the reports given by Zhang et al(IC 50 =0.14 mg/ml) and Gheith and El-Mahmoudy (IC 50 =113 µg/ml) [26,27]. DPPH is usually used as a reagent to evaluate free radical and accepts an electron or hydrogen to become a stable diamagnetic molecule [28].…”

“…The IC 50 values of the EPGL, AA, and GA in this assay were 143 µg/ml, 32.5 µg/ml, and 8.5 µg/ml, respectively. However, Gheith and Mahmoudy reported IC 50 of hydromethanolic extract of PGL as 79.67±0.03 µg/ml [27]. At 100 µg/ml, the inhibition percentage values were 33.6%, 78.6%, and 99.2% for EPGL, AA, and GA, respectively.…”

Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources

“…Importantly, several studies in vitro and in vivo have recorded the superior antioxidant capacity of pomegranate leaves by contrast with its non-edible parts, of which leaves are as effective as peels in the anti-bacterial, analgesic, acute and chronic anti-inflammatory effects 37 , 38 , while more potent than flowers, stems, and seeds 31 , 39 – 42 . Authors proved the potency of pomegranate leaf was higher than that of flower in the prevention of ethylene glycol-induced nephrolithiasis, in the inhibition of DPPH and hydroxyl radicals, and in the reduction of ferric iron 39 , 40 . Data 41 highlighted leaves worked more effectively than stems and led to the most loss of MMP (mitochondrial membrane permeability) potential, consequently suggested as an anti-cancer and anti-proliferative agent.…”

Laboratory evidence for the hematopoietic potential of Beta vulgaris leaf and stalk extract in a phenylhydrazine model of anemia

“…Phytochemical detection tests for presence of phenols and flavonoids in foliage of B. vulgaris were carried out as described previously (22). The tests were performed as triplicates and given marks from (-) to (+++) according to the strength of the color or precipitate that appeared.…”