Potent Anti-Inflammatory Activity of Carbohydrate Polymer with Oxide of Zinc

Abstract

Pebisut is a biological adhesive composed of naturally occurring carbohydrates combined with zinc oxide (ZnO) initially used as a coadjutant for healing of anastomoses. Likewise some works demonstrated that carbohydrate complexes exerts anti-inflammatory activity and it is widely known that ZnO modulate inflammation. However, the direct effects of Pebisut on isolated cells and acute inflammatory responses remained to be investigated. The present study evaluated anti-inflammatory effect of Pebisut using lipopolysaccharide (LPS) stimulated human mononuclear cells, chemotaxis, and cell infiltration in vivo in a murine model of peritonitis. Our data show that human cells treated with different dilutions of Pebisut release less IL-6, IL-1β, and IL-8 after LPS stimuli compared with the control treated cells. In addition, Pebisut lacked chemotactic activity in human mononuclear cells but was able to reduce chemotaxis towards CCL2, CCL5, and CXCL12 that are representative mononuclear cells chemoattractants. Finally, in a murine model of peritonitis, we found less number of macrophages (F4/80+) and T lymphocytes (CD3+) in peritoneal lavages from animals treated with Pebisut. Our results suggest that Pebisut has anti-inflammatory activity, which might have a beneficial effect during anastomoses healing or wounds associated with excessive inflammation.

In vitro bactericidal activity of a carbohydrate polymer with zinc oxide for the treatment of chronic wounds

“…Because CPZO it is not an antibiotic, it is unlikely that it will eventually induce resistance. As mentioned above, the bacteriological findings reported here confirm our clinical impressions over several years of use of CPZO to treat CVUs [9,11], which follows the observation that after several days of CPZO application, wounds no longer have heavy secretions with improvement of the surrounding cellulitis [37]. As an additional advantage, it must be mentioned that after extensive clinical experience with chronic wounds, CPZO does not appear to have any toxic or unpleasant side effects.…”

“…We have previously documented the anti-inflammatory activity of CPZO, which decreases the levels of inflammatory cytokines [9]. Moreover, our clinical observations that infected ulcers become clear after local CPZO application led us to hypothesize that it could possess bactericidal activity.…”

“…As already mentioned, it is now preferred that treatments are free of antibiotics to reduce the risk of resistance. The definite anti-biofilm activity of CPZO adds to its known anti-inflammatory and pro-healing properties [8][9][10][11][12][13][14][15].…”

“…Pebisut is the commercial name in Mexico of a biodegradable adhesive carbohydrate polymer with ZnO (CPZO), which enhances healing and possesses anti-inflammatory activity in patients with CVUs [8]. Although preliminary clinical data suggest that this adhesive mixture decreases infection rates in CVUs, its bactericidal activity remains unknown to date [9][10][11][12][13][14][15]. Therefore, we examined the bactericidal activity of CPZO against Gram-positive and Gram-negative bacteria, all of which frequently complicate chronic wounds.…”

In vivo evaluation of injectable calcium phosphate cement composed of Zn‐ and Si‐incorporated β‐tricalcium phosphate and monocalcium phosphate monohydrate for a critical sized defect of the rabbit femoral condyle

“…At 4 and 8 weeks, IBS 1 and 2 ( Figure 8) showed no initial immunogenic response from fibrous encapsulation and fast degradation. This might be attributed to the anti-inflammatory/antibacterial effect of Zn 21 ions from the implanted sample 34 and physiological degradation behavior of amorphous silica. 35 The antibacterial effect of Zn 1 2 induces faster bone healing by preventing bacterial colonization and degradation of pre mature membrane.…”