POTENSI ANTIOKSIDAN DARI EKSTRAK KULIT BATANG Bauhinia semibifida Roxb

Fadhli, Haiyul; Nurdin, Ainun Nurain; Octaviani, Melzi

doi:10.36387/jiis.v4i1.257

Cited by 6 publications

(8 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study the antioxidant activity assay showed that the IC50 values for the ethanol and methanol extracts were 370.26 ppm and 318.85 ppm, respectively, classifying their antioxidant activity within the weak category. Conversely, vitamin C demonstrated a remarkably low IC50 value of 42.0, indicating a significantly potent antioxidant activity in the very strong category [28]. The observed weak antioxidant activity of the binahong leaf extracts could potentially be attributed to the extraction methodology used.…”

Section: Antioxidant Activity Testmentioning

confidence: 95%

“…The degree of antioxidant potency is quantitatively expressed as the Inhibitory Concentration (IC50) value, which indicates the concentration of the extract required to inhibit 50% of the DPPH radicals or its ability to reduce free radicals by 50%. A lower IC50 value indicates higher antioxidant activity, suggesting the extract's effectiveness in neutralizing free radicals and, consequently, its potential to mitigate oxidative stress and associated health benefits [27], [28]. Antioxidant activity can be categorized into distinct levels of potency, namely: very strong (IC50 < 50 ppm), strong (IC50 between 50-100 ppm), moderate (IC50 between 100-150 ppm), weak (IC50 between 150-200 ppm), and very weak (IC50 > 200 ppm) [29].…”

Section: Antioxidant Activity Testmentioning

confidence: 99%

See 1 more Smart Citation

Phytochemical Screening, Antioxidant and Antifungal Activity Test of Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis)

Sidhartha,

Yang,

Dimara

et al. 2024

EJCHEM

View full text Add to dashboard Cite

Anredera cordifolia (Ten.) Steenis (Binahong) is a medicinal plant that has been used by the indigenous Indonesian community to treat various ailments including surgical wounds, stomach ulcers, and skin problems such as itching and sores. Candida albicans is the most important species responsible for infections in patients with immunological problems associated with invasive fungal diseases. The resistance of Candida species to antifungal drugs has made scientists pay more attention to traditional herbal medicine. Binahong contains secondary metabolites such as alkaloids, flavonoids, polyphenols, saponins, steroids and triterpenoids which have antioxidative and antimicrobial activity. This research was conducted to determine the compounds contained in binahong leaf extract using the Harborne method, determine the antioxidant power of binahong leaf extract through DPPH assay, and its antifungal activity using the Kirby-Bauer disc diffusion method against Candida albicans. The results of the phytochemical screening confirmed that ethanol extract of binahong leaves contains flavonoids, tannins, saponins and steroid compounds. Meanwhile, methanol extract of binahong leaves contains saponin and steroid compounds. The antioxidant test using the DPPH method showed that ethanol and methanol binahong leaf extracts had weak antioxidant activity, with IC50 values of 370.26 ppm for ethanol extract and 318.85 ppm for methanol extract. Both the ethanol and methanol extracts displayed significant anfungal activity against Candida albicans, with extract concentration as low as 6.25%. With the results obtained, it is suggested that ethanol and methanol extracts can be used as antioxidants and antifungals.

show abstract

Section: Antioxidant Activity Testmentioning

confidence: 95%

Section: Antioxidant Activity Testmentioning

confidence: 99%

Phytochemical Screening, Antioxidant and Antifungal Activity Test of Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis)

Sidhartha,

Yang,

Dimara

et al. 2024

EJCHEM

View full text Add to dashboard Cite

show abstract

“…The DPPH scavenging activity assay was conducted following the method mentioned in Fadhli et al, (2019) with a slight modification, where, 11 concentration of standards were used instead of six standards. The ascorbic acid was used as a positive control in this experiment.…”

Section: Dpph Radical Scavenging Assaymentioning

confidence: 99%

Toxicity of Anacardium occidentale shoots (Pucuk Gajus) Extract on Zebrafish Embryos

Haron,

Tajudin,

Abdul Wahab

et al. 2023

RAS

View full text Add to dashboard Cite

The use of various medicinal plant to remedy specific disease has become more popular and grown intensively due to the preference for using medicinal plant instead of synthetic drugs. However, it is vital to know the safe dose limit of natural products consumption. In this present study, the medicinal plant such as Anacardium occidentale (cashew) shoots which is commonly consumed as ulam or as a salad in some Asian countries such as Malaysia and Indonesia was used. This study aims to evaluate the antioxidant capacity of the cashew shoots by using 70% methanolic:water and to determine its toxicity effect on the Danio rerio (zebrafish) embryos. The antioxidant capacity was calculated in IC50 value where the A. occidentale methanolic extract was found to have higher antioxidant activity (IC50 = 4.02 ± 0.02 ?g/mL) as compared to ascorbic acid (7.44 ± 0.02 ?g/mL) (p<0.05). At higher concentration of A. occidentale (9.802 ?g/mL), it showed the toxicity effects on the mortality, hatchability, and several deformations (spinal curve, heart complication, and the presence of oedema). Therefore, it is recommended to use lower concentration of extract to prevent toxicity.Keywords: A. occidentale, Danio rerio (zebrafish) embryos, antioxidants

show abstract

“…diperoleh senyawa fenolik 6C-7O-dimethylaromadendrin dan phlorizin yang memiliki aktivitas sitotosik terhadap sel Murin Leukimia P-388 dengan IC50 sebesar 72,93 ppm. Selain itu, pada penelitian Fadhli et al (2019) menunjukkan bahwa ekstrak metanol kulit batang B. semibifida Roxb. memiliki aktivitas antioksidan yang kuat dengan nilai IC50 sebesar 16 µg/mL.…”

Section: Pendahuluanunclassified

“…Berdasarkan Tabel 1 dan gambar 1, senyawa murni BR2 dan BR3 memiliki aktivitas sangat kuat sebagai antioksidan dengan nilai IC50 sebesar 2,92 µg/mL dan 4,39 µg/mL dibandingkan pada penelitian sebelumnya yang menunjukkan bahwa ekstrak metanol kulit batang B. semibifida Roxb. memiliki IC50 sebesar 16 µg/mL (Fadhli et al, 2019). Perbedaan aktivitas antioksidan senyawa BR2 dan BR3 yang merupakan senyawa murni dibanding dengan ekstrak metanol, dikarenakan ekstrak metanol mengandung lebih dari satu senyawa aktif yang memungkinkan adanya interaksi pada senyawa ekstrak tersebut, di mana interaksi bersifat antagonis ataupun sinergis (Prieto et al, 2014).…”

Section: Analisis Dataunclassified

Isolasi Senyawa Metabolit Sekunder dari Ekstrak Metanol Kulit Batang Bauhinia semibifida Roxb. dan Uji Aktivitas Antioksidan

Fadhli¹,

Ummah²,

Rahmawati³

2021

ALCHEMY J.Pen.Kim

Self Cite

View full text Add to dashboard Cite

Kulit batang Bauhinia semibifida Roxb. telah lama digunakan sebagai obat tradisional oleh masyarakat Melayu Lingga, Kepulauan Riau. Penelitian ini bertujuan untuk mengisolasi dan menguji aktivitas antioksidan senyawa metabolit sekunder dari ekstrak metanol kulit batang B. semibifida Roxb. Tiga isolat murni berupa satu senyawa alkaloid (BR1) dan dua senyawa flavonoid (BR2 dan BR3) telah berhasil diisolasi dengan cara ekstraksi menggunakan metode maserasi dan dilanjutkan fraksinasi dengan metode kromatografi kolom. Uji aktivitas antioksidan dilakukan terhadap isolat dengan metode 2,2 difenil-1-pikrilhidrazil (DPPH). Senyawa isolat BR1 menunjukan IC50 >1000 µg/mL dikategorikan tidak aktif sebagai antioksidan, sedangkan senyawa isolat BR2 dan BR3 menunjukan IC50 2,92 µg/mL dan 4,39 µg/mL dikategorikan sangat kuat sebagai antioksidan jika dibandingkan dengan vitamin C sebagai kontrol. Berdasarkan evaluasi aktivitas antioksidan senyawa BR2 adalah senyawa pilihan yang dilanjutkan identifikasi dengan spektroskopi Fourier-Transform Infrared Spectroscopy (FTIR) dan Proton Nuclear Magnetic Resonance (1H-NMR) sehingga diduga senyawa isolat memiliki struktur dasar senyawa flavonoid.Isolation of Secondary Metabolite Compounds of Methanolic Extract of Steam Bark Bauhinia semibifida Roxb. and Their Antioxidant Activites. Stem bark of Bauhinia semibifida Roxb. has been long consumed as a medicinal herb by Lingga Malaya Ethnic. Therefore, this research aimed to isolate and examines the antioxidant activity of secondary metabolites from methanol extract of stembark of Bauhinia semibifida Roxb. Three pure compounds which are an alkaloid (BR1) and two flavonoids (BR2 and BR3) have been isolated by extraction using maceration method followed by fractionation using column chromatography method. Antioxidant activity assay was carried out by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) method. Compound BR1 showed IC50 >1000 µg/mL categorized as inactive as an antioxidant, while compounds BR2 and BR3 showed IC50 2.92 µg/mL and 4.394 µg/mL, respectively, categorized as very strong as an antioxidant when compared to vitamin C as a control. Based on the evaluation of the antioxidant activity, the BR2 was selected compound and continued to identification by Fourier-Transform Infrared Spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy so that the isolate has the basic structure of flavonoid compound.

show abstract

POTENSI ANTIOKSIDAN DARI EKSTRAK KULIT BATANG Bauhinia semibifida Roxb

Cited by 6 publications

References 1 publication

Phytochemical Screening, Antioxidant and Antifungal Activity Test of Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis)

Phytochemical Screening, Antioxidant and Antifungal Activity Test of Binahong Leaf Extract (Anredera cordifolia (Ten.) Steenis)

Toxicity of Anacardium occidentale shoots (Pucuk Gajus) Extract on Zebrafish Embryos

Isolasi Senyawa Metabolit Sekunder dari Ekstrak Metanol Kulit Batang Bauhinia semibifida Roxb. dan Uji Aktivitas Antioksidan

Contact Info

Product

Resources

About