Potency of Vitamin B1 Preparations

On the ultra-violet absorption of crystalline preparations of vitamin B 1

“…It is therefore somewhat surprising that the preparations recently made at Oxford (Kinnersley, O'Brien andPeters (1932-1933)), which can claim to be the most potent yet produced, have their maximum absorption at a definitely different wave-length, namely 245-9 mjji, while at 260mfji their absorption curves only show a barely perceptible hump, protruding from the steeply descending portion of the main band. An attem pt to connect this discrepancy with a possible effect of hydrion concentration on the wave-length of the jti a.ximnm led to the discovery of a curious phenomenon.…”

“…Fig. 1 shows e/C for :-(a) The preparation 61A (15) described by Kinnersley, O'Brien, and Peters (1933), dissolved in absolute alcohol containing N/200 HC1.…”

“…It is therefore somewhat surprising that the preparations recently made at Oxford (Kinnersley, O'Brien and Peters (1932-1933)), which can claim to be the most potent yet produced, have their maximum absorption at a definitely different wave-length, namely 245-9 mjji, while at 260mfji their absorption on May 10, 2018 http://rspb.royalsocietypublishing.org/ Downloaded from…”

“…Some tests per formed (a) with the actual solutions used, and (6) with some similarly treated support this view. Previous to treatment, the activities were for 64 (19) 2-2 ±0-97y and for 61A (15) l-6±0*46y, curative day dose for pigeon (see Kinnersley, O'Brien and Peters, 1933).…”