Potency of Nuisance Flies as Vector of Coxiella burnetii on Dairy Farms in Ampel and Mojosongo Districts, Boyolali Regency, Middle Java, Indonesia

Abstract

This study aimed to determine the characteristics, distribution and diversity of nuisance flies in dairy farms at Ampel and Mojosongo Districts, Boyolali Regency. The Q fever incidence in dairy cattle and the nuisance flies potency as vectors of Q fever were also examined, considering that previous studies found positive cases of Q fever in 4 beef cattle slaughtered at the Ampel Slaughterhouse, Ampel District, Boyolali Regency. The collection of flies was carried out using the sweep net and the Nzi Trap. A total of 351 nuisance flies were collected, analyzed, and as many as 200 flies were examined by nested-PCR with OMP1, OMP2, OMP3 and OMP4 primer. We also collected and tested milk samples (20 samples) with nested-PCR. The results showed no amplified DNA band (437 bp) in both milk and fly samples. It indicated that there is no evidence of pathogenic infection causing Q fever in dairy cattle at Boyolali Regency and the nuisance flies have not been shown to act as a vector of C. burnetii.