Potency Of Bacteriocin For Animal Health And Food Safety

Chotiah, Siti

doi:10.14334/wartazoa.v23i2.719

Cited by 2 publications

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“…E-mail address: b_jeanne@batan.go.id treated by antibiotics application, which is primarily eliminates bacteria. However, the uncontrolled use of antibiotics in the field may leads to new problems of the resistant germs-caused mastitis to antibiotics and drugs residue in milk [6]. In this situation, difficulty is found in bacteria caused mastitis eradication, and antibiotic residues in milk may appear and can be harmful to human health.…”

Section: Introduction * * * *mentioning

confidence: 99%

Bacterial Protein Characterization of <em>Streptococcus agalactiae</em> by SDS-page Method for Subclinical Mastitis Irradiated Vaccine Materials in Dairy Cattle

et al. 2013

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A study have been conducted to isolate and characterize bacterial protein S. agalactiae, which is antigenic and can be used to test immunogenicity of vaccine in order to manufacture irradiated mastitis (inflammation of the udder) vaccine in ruminant. The study aims to determine the Molecular Weight (MW) bacterial protein S. agalactiae irradiation, which can be used to test the nature of its antigenic caharacteristic. The character of S. agalactiae antigenic stimulates antibody induction of the immune system, in which case is the body's defense system against mastitis disease in cattle. In this study, irradiation of gamma ray is used to attenuate the pathogenicity of bacteria by reducing S. agalactiae antigenic caharacteristic. Previous research, in irradiation dose orientation before antigenic protein isolation of S. agalactiae, indicated that irradiation lethal dose to 50% (LD 50 ) is 17 Gy. The characterization of S. agalactiae bacteria isolate using SDS-page method results in no significance different between irradiated and non-irradiated group, which indicated by MW range 75 -100 kDa base on marker standard which used, or 99 kDa by the linier equation of Y = 11,60 -0.05X (where Y = bands distance; X = MW standard protein); r 2 = 0.99. In conclusion, 17 Gy irradiation dose does not impair antigenic property of S. agalactiae and therefore, can be applied to produce base material of irradiated vaccine for mastitis.

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Section: Introduction * * * *mentioning

confidence: 99%

Bacterial Protein Characterization of <em>Streptococcus agalactiae</em> by SDS-page Method for Subclinical Mastitis Irradiated Vaccine Materials in Dairy Cattle

et al. 2013

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show abstract

“…Most of the mastitis was treated by antibiotics application, which is primarily eliminates bacteria. However, the uncontrolled use of antibiotics in the field may leads to new problems of the resistant pathogenic strains to antibiotics and drugs residue in milk (Chotiah, 1993).…”

Section: Introductionmentioning

confidence: 99%