Potato Stu-miR398b-3p Negatively Regulates Cu/Zn-SOD Response to Drought Tolerance

Abstract

One of the main impacts of drought stress on plants is an excessive buildup of reactive oxygen species (ROS). A large number of ·OH, highly toxic to cells, will be produced if too much ROS is not quickly cleared. At the heart of antioxidant enzymes is superoxide dismutase (SOD), which is the first antioxidant enzyme to function in the active oxygen scavenging system. To shield cells from oxidative injury, SOD dismutation superoxide anion free radicals generate hydrogen peroxide and molecule oxygen. Cu/Zn SOD is a kind of SOD antioxidant enzyme that is mostly found in higher plants’ cytoplasm and chloroplasts. Other studies have demonstrated the significance of the miR398s family of miRNAs in the response of plants to environmental stress. The cleavage location of potato stu-miR398b-3p on Cu/Zn SOD mRNA was verified using RLM-5′RACE. Using the potato variety ‘Desiree’, the stu-miR398b-3p overexpression mutant was created, and transgenic lines were raised. SOD activity in transgenic lines was discovered to be decreased during drought stress, although other antioxidant enzyme activities were mostly unaltered. Transgenic plants will wilt more quickly than wild-type plants without irrigation. Additionally, this demonstrates that the response of Cu/Zn SOD to drought stress is adversely regulated by potato stu-miR398b-3p.

Identification and characterization of the critical genes encoding Cd-induced enhancement of SOD isozymes activities in Zhe-Maidong (Ophiopogon japonicus)

“…On the other hand, it possibly resulted from regulation by metabolites or other regulatory factors (Chu et al, 2005;Du et al, 2023;Zheng et al, 2023). Overall, these results validate that Cu/ ZnSODa and Cu/ZnSODb were the primary components responsible for the rise in total SOD enzyme activity when subjected to Cd stress and played a crucial role in the scavenging of Cd stress-induced ROS damage.…”

“…Here, we supposed that tissue-specific expression of SOD isozymes helps to maintain the ROS balance, ensuring proper cellular function in the leaf or root with distinct metabolic demands ( Che et al., 2020 ). On the other hand, it possibly resulted from regulation by metabolites or other regulatory factors ( Chu et al., 2005 ; Du et al., 2023 ; Zheng et al., 2023 ). Overall, these results validate that Cu/ZnSODa and Cu/ZnSODb were the primary components responsible for the rise in total SOD enzyme activity when subjected to Cd stress and played a crucial role in the scavenging of Cd stress-induced ROS damage.…”

Overexpression of the late embryonic genesis abundant protein MGL3 improves the drought tolerance of maize (Zea mays L.)

“…Nonetheless, plants have evolved effective ROS‐scavenging defense mechanisms (Zandalinas & Mittler, 2018) and use enzymes, such as SOD (Woith et al., 2017), POD (Livanos et al., 2017), and CAT (Huang et al., 2019), to protect themselves against oxidative stress‐induced cell damage (A. Singh et al., 2023). According to previous studies, SOD prevents membrane damage related to oxidative stress in plants (Zheng et al., 2023); hence, it can be reasonably inferred that SOD concentration is positively correlated with membrane damage caused by abiotic stress. Additionally, ROS is also an important signaling molecule that regulates many biological processes (Schneider et al., 2019), and many studies have shown that the scavenging ability of ROS is related to plant tolerance to abiotic stresses (Li et al., 2022; M. Liang et al., 2022).…”