Potato (Solanum tuberosum L.)

Chetty, Venkateswari J.; Narváez-Vásquez, Javier; Orozco-Cárdenas, Martha L.

doi:10.1007/978-1-4939-1658-0_8

Cited by 13 publications

(4 citation statements)

References 17 publications

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“…Culture preparation, transformation and regeneration of potato plants were performed according to Chetty et al . (). Pathogen‐free nuclear stocks of S. tuberosum cv.…”

Section: Methodsmentioning

confidence: 97%

Cas9‐mediated mutagenesis of potato starch‐branching enzymes generates a range of tuber starch phenotypes

Tuncel

Corbin

Ahn‐Jarvis

et al. 2019

Plant Biotechnology Journal

119

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Summary We investigated whether Cas9‐mediated mutagenesis of starch‐branching enzymes (SBEs) in tetraploid potatoes could generate tuber starches with a range of distinct properties. Constructs containing the Cas9 gene and sgRNAs targeting SBE1, SBE2 or both genes were introduced by Agrobacterium‐mediated transformation or by PEG‐mediated delivery into protoplasts. Outcomes included lines with mutations in all or only some of the homoeoalleles of SBE genes and lines in which homoeoalleles carried several different mutations. DNA delivery into protoplasts resulted in mutants with no detectable Cas9 gene, suggesting the absence of foreign DNA. Selected mutants with starch granule abnormalities had reductions in tuber SBE1 and/or SBE2 protein that were broadly in line with expectations from genotype analysis. Strong reduction in both SBE isoforms created an extreme starch phenotype, as reported previously for low‐SBE potato tubers. HPLC‐SEC and 1H NMR revealed a decrease in short amylopectin chains, an increase in long chains and a large reduction in branching frequency relative to wild‐type starch. Mutants with strong reductions in SBE2 protein alone had near‐normal amylopectin chain‐length distributions and only small reductions in branching frequency. However, starch granule initiation was enormously increased: cells contained many granules of <4 μm and granules with multiple hila. Thus, large reductions in both SBEs reduce amylopectin branching during granule growth, whereas reduction in SBE2 alone primarily affects numbers of starch granule initiations. Our results demonstrate that Cas9‐mediated mutagenesis of SBE genes has the potential to generate new, potentially valuable starch properties without integration of foreign DNA into the genome.

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“…Culture preparation, transformation and regeneration of potato plants were performed according to Chetty et al . (). Pathogen‐free nuclear stocks of S. tuberosum cv.…”

Section: Methodsmentioning

confidence: 97%

Cas9‐mediated mutagenesis of potato starch‐branching enzymes generates a range of tuber starch phenotypes

Tuncel

Corbin

Ahn‐Jarvis

et al. 2019

Plant Biotechnology Journal

119

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“…Other studies indicate that SMEs operating in highly competitive and uncertain domestic environments look to export markets as opportunities to expand their operation base (Dimitratos, Lioukas, and Carter 2004). The role of networks for SMEs pursuing growth in international markets is well-studied (Chetty and Stangl 2010;Johanson and Vahlne 2009). Networks help to reduce risks by reducing liability of newness, establishing legitimacy (Deeds, Mang, and Frandsen 2004;Elfring and Hulsink 2003) and decreasing supply and distribution costs (Li and Zhou 2010;Sheng, Zhou, and Li 2011).…”

Section: Introductionmentioning

confidence: 99%

Influence of Innovativeness, Environmental Competitiveness and Government, Industry and Professional Networks on SME Export Likelihood

Chang

Webster

2018

Journal of Small Business Management

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The purpose of this study is to examine how small to medium‐sized enterprises (SMEs) in different industry segments utilize government, industry, and professional networks and to test the influence of SME innovativeness, environmental competitiveness and network type for SME export likelihood. Previous studies on networks and exports tend to be qualitative and based on small samples. This study adds to previous scholarship by investigating a large database consisting of 2,263 Australian SMEs. Results indicate SME innovativeness contributes positively to export likelihood while contrary to extant studies, no significant relationship is observed between environmental competitiveness and export likelihood. Moreover, a significant, positive relationship exists between government networks and export likelihood, and a positive though weaker relationship between industry networks and export likelihood. On average, the likelihood of SMEs receiving export income increases by 47 percent if all three network types are accessed between one and three times a year, controlling for other factors. Further analyses of industry sub‐segments indicate a stronger relationship between industry networks and export likelihood.

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“…Figure 1: Meristem culture as a source for accelerated early generation seed (EGS) production techniques at Holetta Research Centre (Source: Many scholars perform in vitro manipulation technology in potato varying from the low-input and widely adopted technology of micropropagation to the complex manipulation of multi-gene biosynthetic pathways through transgenic intervention. Potato is considered as one of a model species for methods such as somatic hybridization due to its in vitro regeneration capacity to overcome the sexual barriers between the cultivated (Solanum tuberosum L.) and wild species (Melchers et al, 1978;Tiwari et al, 2018) and Agrobacteriummediated transformation (Conner et al, 1992;Millam, 2007;Cingel et al, 2010;Chetty et al, 2015). Several improvements have been made for more than two decades with respect to tissue culture, transformation, and regeneration of potato (Bruce and Shoup Rupp, 2019).…”

Section: Importance Of Plant Tissue Culturementioning

confidence: 99%

Microbial Contaminants and Their Control Methods: A Review with Reference to Potato Tissue Culture

2021

JNSR

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No cell or tissue culture problem is as universal as that of culture loss due to contamination, so that microbial hazards cause drastic economic losses in the plant tissue culture industries. A wide range of microorganisms (filamentous fungi, yeasts, bacteria, virus and viroid) and micro-arthropods (mites and thrips) have been identified as contaminants in plant tissue culture. With this paramount importance of in-vitro culture contaminants, various research work has been conducted from different corners of the world. This review paper compiles such important information to share experiences within a single amassed document. Different research and review articles, proceedings, protocol notes, scientific notes, conference papers, case reports as well as case studies, research communications and technical reports are included in this paper. Hence, this article aimed to review and provide insights about microbial contaminants and their management techniques and thereby to document the possible attempts made by different scholars to mitigate microbial contamination under plant tissue culture. Moreover, the paper skim through the current advancements made on culture loss due to microbes, their control methods and hostile effects on regeneration capability of culture plants.

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Potato (Solanum tuberosum L.)

Cited by 13 publications

References 17 publications

Cas9‐mediated mutagenesis of potato starch‐branching enzymes generates a range of tuber starch phenotypes

Cas9‐mediated mutagenesis of potato starch‐branching enzymes generates a range of tuber starch phenotypes

Influence of Innovativeness, Environmental Competitiveness and Government, Industry and Professional Networks on SME Export Likelihood

Microbial Contaminants and Their Control Methods: A Review with Reference to Potato Tissue Culture

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