Potato plants bearing a vacuolar Na+/H+ antiporter HvNHX2 from barley are characterized by improved salt tolerance

Bayat, Fereshteh; Shiran, Behrouz; Belyaev, D. V.; Yur’eva, N. O.; Sobolkova, G. I.; Alizadeh, Houshang; Khodambashi, M.; Бабаков, А. В.

doi:10.1134/s1021443710050134

Cited by 24 publications

(7 citation statements)

References 22 publications

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“…Similar results were also reported by Zhao et al (2007) and Wei et al (2010). The over-expression of HvNHX2 from barley in transgenic potato (Bayat et al 2010) affected ion accumulation in a similar way to that of the over-expression of TNHXS1 in transgenic tobacco (this study). Interestingly, sequence analysis revealed 99 % similarity between HvNHX2 and TNHXS1.…”

Section: Discussionsupporting

confidence: 90%

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

et al. 2012

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Abiotic stress tolerance of plants is a very complex trait and involves multiple physiological and biochemical processes. Thus, the improvement of plant stress tolerance should involve pyramiding of multiple genes. In the present study, we report the construction and application of a bicistronic system, involving the internal ribosome entry site (IRES) sequence from the 5'UTR of the heat-shock protein of tobacco gene NtHSF-1, to the improvement of salt tolerance in transgenic tobacco plants. Two genes from wheat encoding two important vacuolar ion transporters, Na(+)/H(+) antiporter (TNHXS1) and H(+)-pyrophosphatase (TVP1), were linked via IRES to generate the bicistronic construct TNHXS1-IRES-TVP1. Molecular analysis of transgenic tobacco plants revealed the correct integration of the TNHXS1-IRES-TVP1construct into tobacco genome and the production of the full-length bicistronic mRNA from the 35S promoter. Ion transport analyses with tonoplast vesicles isolated from transgenic lines confirmed that single-transgenic lines TVP1cl19 and TNHXS1cl7 had greater H(+)-PPiase and Na(+)/H(+) antiport activity, respectively, than the WT. Interestingly, the co-expression of TVP1 and TNHXS1 increased both Na(+)/H(+) antiport and H(+)-PPiase activities and induced the H(+) pumping activity of the endogenous V-ATPase. Transgenic tobacco plants expressing TNHXS1-IRES-TVP1 showed a better performance than either of the single gene-transformed lines and the wild type plants when subjected to salt treatment. In addition, the TNHXS1-IRES-TVP1 transgenic plants accumulated less Na(+) and more K(+) in their leaf tissue than did the wild type and the single gene-transformed lines. These results demonstrate that IRES system, described herein, can co-ordinate the expression of two important abiotic stress-tolerance genes and that this expression system is a valuable tool for obtaining transgenic plants with improved salt tolerance.

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Section: Discussionsupporting

confidence: 90%

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

et al. 2012

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“…The control plants grown on uranyl had long and curved roots and petioles. This stretching of plant organs had been reported by us [ 28 ] and elsewhere. The cotyledonous leaves of control plants were small and bent downward (see Figure 5 b).…”

Section: Resultssupporting

confidence: 84%

“…For DNA and RNA isolations, plants were grown from seeds in Magenta boxes on MS medium with 0.5% sucrose and 50 mg/L Km. RNA was isolated as described in [ 28 ]. For Northern hybridizations, RNA was blotted to Amersham Hybond-N+ membrane (GE Healthcare, NY, USA) and probed with XbaI-SacI fragment containing cytc3 coding region that was 32 P-labelled by NEBlot kit (New England Biolabs, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco

Беляев

Терешонок

Lunkova

et al. 2021

IJMS

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Cytochrome c3 (uranyl reductase) from Desulfovibrio vulgaris can reduce uranium in bacterial cells and in cell-free systems. This gene was introduced in tobacco under control of the RbcS promoter, and the resulting transgenic plants accumulated uranium when grown on a uranyl ion containing medium. The uptaken uranium was detected by EM in chloroplasts. In the presence of uranyl ions in sublethal concentration, the transgenic plants grew phenotypically normal while the control plants’ development was impaired. The data on uranium oxidation state in the transgenic plants and the possible uses of uranium hyperaccumulation by plants for environmental cleanup are discussed.

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“…Furthermore, transgenic barley AVP1 genotypes had a higher yield as indicated by a larger number of heads per plant (16-58%), grains per plant (76-85%), average grain weight (29-43%), and grain yield per plant (79-87%) (Schilling et al 2014). A third vacuolar Na + /H + antiporter, HvNHX2, was isolated in barley (Vasekina et al 2005) and increased salinity tolerance was observed when HvNHX2 was overexpressed in arabidopsis (Bayat et al 2011) and potato plants (Bayat et al 2010).…”

Section: Soil Salinitymentioning

confidence: 99%

Genomic and Genetic Studies of Abiotic Stress Tolerance in Barley

Saadé

Negrão

Plett

et al. 2018

Compendium of Plant Genomes

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Barley is a resilient crop plant with higher tolerance than other cereal plants for several types of abiotic stress. In this chapter, we describe the genetic components underlying barley's response to abiotic stresses, including soil acidity, boron toxicity, soil salinity, drought, temperature, and nutrient deficiency. We describe typical symptoms observed in barley in response to these stresses. We enumerate the major qualitative trait loci (QTLs) identified so far, such as FR-H1 and FR-H2 for low-temperature tolerance. We also discuss candidate genes that are the basis for stress tolerance, such as HVP10, which underlies the HvNax3 locus for salinity tolerance. Although knowledge about barley's responses to abiotic stresses is far from complete, the genetic diversity in cultivated barley and its close wild relatives could be further exploited to improve stress tolerance. To this end, the release of the barley high-quality reference genome provides a powerful tool to facilitate identification of new genes underlying barley's relatively high tolerance to several abiotic stresses. Early research on the genetic control of Al tolerance in barley using the Dayton (Al-tolerant) and Smooth Awn 86 (Al-sensitive) genotypes detected a major locus, Alp, on the long arm of chromosome 4H (4HL) (Minella and Sorrells 1992; Reid 1971). Another early study found that a single gene, named Pht, on

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Potato plants bearing a vacuolar Na+/H+ antiporter HvNHX2 from barley are characterized by improved salt tolerance

Cited by 24 publications

References 22 publications

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

Expression of wheat Na+/H+ antiporter TNHXS1 and H+- pyrophosphatase TVP1 genes in tobacco from a bicistronic transcriptional unit improves salt tolerance

Expression of Cytochrome c3 from Desulfovibrio vulgaris in Plant Leaves Enhances Uranium Uptake and Tolerance of Tobacco

Genomic and Genetic Studies of Abiotic Stress Tolerance in Barley

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