Potato leafroll virus contains a double-stranded DNA

Sarkar, S.

doi:10.1016/0042-6822(76)90269-5

Cited by 24 publications

(9 citation statements)

References 17 publications

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“…PLRV has been temporarily excluded from the luteovirus group because its nucleic acid was erroneously reported to be DNA (Sarkar, 1976). However, it has now been established that PLRV has single-stranded RNA of similar mol.…”

Section: Discussionmentioning

confidence: 99%

Luteovirus-like Particles Associated with Subterranean Clover Red Leaf Virus Infection

Jayasena¹,

Hatta

Francki

et al. 1981

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Luteovirus-like Particles Associated with Subterranean Clover Red Leaf Virus Infection

Jayasena¹,

Hatta

Francki

et al. 1981

Journal of General Virology

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“…6 was transferred from an infected potato plant to Physalisfloridana with the help of the aphid vector Myzus persicae Sulz and maintained in an aphid-proof greenhouse. The virus was purified from leaves and stems of infected Physalis/loridana plants as described by Sarkar [35].…”

Section: Preparation Of Virus Particlesmentioning

confidence: 99%

Cloning of the gene for the capsid protein of potato leafroll virus

Tacke¹,

Sarkar

Salamini³

et al. 1989

Archives of Virology

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DNA complementary to the RNA of purified potato leafroll virus (PLRV) was synthesized and cloned into the lambda insertion vector NM1149. Overlapping PLRV-specific cDNA clones were isolated that represent some 80% of the viral genome. Sequences coding for the capsid protein were identified by subcloning size-selected cDNAs into the lambda expression vector gt11 and screening with PLRV-specific antisera. The gene for the viral capsid protein was shown to reside in the 3' terminal half of the genomic RNA. Sequence comparisons with the recently published genomes of the beet western yellows virus (BWYV) and the barely yellow dwarf virus (BYDV) reveal some 65% protein sequence homology between the capsid proteins of BWYV and PLRV and some 45% homology between BYDV and PLRV. Furthermore, it is evident that the structural organization of the PLRV genome in the CP gene region is similar to that of BWYV and BYDV.

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“…PLRV has been purified by Peters (1967a and b), Kojima et al (1969), Murayama and Kojima (1974), and Sarkar (1975Sarkar ( , 1976. Successful antiserum preparation has been reported by Kojima and Murayama (1972) and Murayama and Kojima (1974), Their antiserum has been used by Caspar (1977) in his ELISA experiments with the virus.…”

Section: Introductionmentioning

confidence: 97%

Potato leafroll virus: antiserum preparation and detection in potato leaves and sprouts with the enzyme-linked immunosorbent assay (ELISA)

Maat¹,

Bokx²

1978

Netherlands Journal of Plant Pathology

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Potato leafroll virus (PLRV) was purified from Physalis floridana, applying freezing, low-speed centrifuging, ammonium sulphate precipitation, clarification with chloroform and butanol, ultracentrifuging and sucrose-gradient centrifuging. Three antisera with titers from 64 to 256 were prepared, one of them being sufficiently specific to be used in the enzyme-linked immunosorbent assay (ELISA).With this test PLRV could be detected reliably in the foliage of secondarily infected, glasshousegrown potato plants of six cultivars tested, and in sprouts of four or five of them. The results indicate that ELISA may be used successfully for routine testing of foliage of glasshouse-grown potato plants.

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Potato leafroll virus contains a double-stranded DNA

Cited by 24 publications

References 17 publications

Luteovirus-like Particles Associated with Subterranean Clover Red Leaf Virus Infection

Luteovirus-like Particles Associated with Subterranean Clover Red Leaf Virus Infection

Cloning of the gene for the capsid protein of potato leafroll virus

Potato leafroll virus: antiserum preparation and detection in potato leaves and sprouts with the enzyme-linked immunosorbent assay (ELISA)

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