Potato late blight field resistance from QTL dPI09c is conferred by the NB-LRR gene R8

Abstract

Using genetics, genomics, and diagnostic RenSeq, we demonstrate that the major NB-LRR gene R8 explains the field resistance against potato late blight in the QTL dPI09c

Tracking disease resistance deployment in potato breeding by enrichment sequencing

“…PCR-based tests are typically unable to identify such variations without sequencing multiple cloned products (Van Weymers et al, 2016). Indeed, as recently shown for R8, positive PCR amplification does not necessary indicate the presence of functional NLRs as pseudogenised genes can also yield products of similar size (Jiang et al, 2018). Therefore, it is often necessary to Sanger sequence PCR products of NLRs, which typically requires the cloning of PCR products and sequencing of recombinant clones.…”

“…The methodology takes advantage of the focused re-sequencing of NLRs through RenSeq (Jupe et al, 2013). RenSeq has previously been used for improving genome annotations and genetic mapping of plant NLRs (Chen et al, 2018;Jupe et al, 2013), the prioritisation of novel NLRs in wild diploid species (Jiang et al, 2018;Van Weymers et al, 2016), and identification of candidate NLRs when combined with long-read sequencing technology (Giolai et al, 2016;Witek et al, 2016).…”

South American species <i>Solanum alandiae</i> Card. and <i>S. okadae</i> Hawkes et Hjerting as potential sources of genes for potato late blight resistance

“…Following the marker-assisted mapping and cloning of Rpi genes, recent years have seen a considerable progress in this direction. New methodologies, such as effectoromics, allele profiling and diagnostic resistance gene enrichment sequencing (dRenSeq), tremendously facilitated the search for new Rpi genes and new alleles of Rpi genes already characterized in other species of Solanum L. within the section Petota Dumort van Weymers et al, 2016;Chen et al, 2018;Jiang et al, 2018;Armstrong et al, 2019). Currently some of these genes have been introgressed into commercial potato cultivars (Haesaert et al, 2015).…”

Tracking disease resistance deployment in potato breeding by enrichment sequencing

“…The methodology takes advantage of the focused re-sequencing of NLRs through RenSeq (Jupe et al, 2013). RenSeq has previously been used for improving genome annotations and genetic mapping of plant NLRs (Jupe et al, 2013;Chen et al, 2018), the prioritisation of novel NLRs in wild diploid species (Van Weymers et al, 2016;Jiang et al, 2018), and of candidate NLRs in combination with long-read sequencing technology (Giolai et al, 2016;Witek et al, 2016).…”