Potato Haploids and Breeding

Rokka, Veli-Matti

doi:10.1007/978-1-4020-8854-4_17

Cited by 33 publications

(23 citation statements)

References 39 publications

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“…At present, refined protocols have been developed to produce DHs from tobacco anther and isolated microspore cultures on a routine basis and with an acceptable efficiency (reviewed in Belogradova et al 2009). Potato cannot be considered as a model system, but methods to induce haploids, from both anther and microspore culture, are already available (reviewed in Rokka 2009). …”

Section: Introductionmentioning

confidence: 99%

Taxonomy and genetic diversity of domesticated Capsicum species in the Andean region

Ibiza

Blanca

Cañizares

et al. 2011

Genet Resour Crop Evol

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 Abstract Tomato, eggplant and pepper are three solanaceous crops of outstanding importance worldwide. Thus, a fast and cheap method to produce pure (homozygous) lines is a priority for breeders. Traditionally, pure lines are produced by classical inbreeding and selection techniques, which are time consuming (several years) and costly. Alternatively, it has become possible to accelerate the production of homozygous lines through a biotechnological approach: the induction of androgenesis to generate doubled haploid (homozygous) plants. This biotechnological in vitro tool reduces the process to only one generation, which implies important time and costs savings. These facts make androgenic doubled haploids the choice in a number of important crops where the methodology is well set up. Unfortunately, recalcitrant solanaceous crops such as tomato, eggplant and pepper are still far from an efficient and reliable technology to be applied to different genotypes in breeding programs on a routine basis. In eggplant and pepper, only anther cultures are known to work relatively well. Unfortunately, a more efficient and promising technique, the culture of isolated microspores, is not sufficiently developed yet. In tomato, none of these methods is available. However, recent advances in the knowledge of zygotic and androgenic embryo development are filling the gaps and opening new ways to achieve the final goal of an efficient protocol in these three recalcitrant species. In this review, we outline the state of the art on androgenic induction in tomato, eggplant and pepper, and postulate new experimental ways in order to overcome current limitations.

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Section: Introductionmentioning

confidence: 99%

Taxonomy and genetic diversity of domesticated Capsicum species in the Andean region

Ibiza

Blanca

Cañizares

et al. 2011

Genet Resour Crop Evol

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“…These haploid clones are of interest for both genetic and genomic studies currently underway in our research group as well as for potato breeding. Haploids plants obtained from Solanum species are useful tools for breeding and genetic research (Rokka et al 2009). …”

Section: Importance Of Anther Culture In Potato Research and Breedingmentioning

confidence: 99%

“…It also has selfincompatibility at the diploid level (Bradshaw 2007). Despite the novel developments in genetic mapping using an inbred line-derived F2 population (Endelman and Jansky 2016), dihaploids and monoploids are still the tools of choice for genetic, cytogenetic and evolutionary studies (Rokka 2009). In the context of the Potato Genome Sequencing Consortium (PGSC 2011), the doubled monoploid clone (DM1-3 516R44) developed by Veilleux et al (1995) allowed a reference assembly, because the DM1-3 clone is a completely homozygous line, which eliminates the complexity brought into the assembly by heterozygosity (Visser et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Gametic Embryogenic Response in Wild Diploid Solanum Species and its Implications for Genome Sequencing Projects and Breeding

Castillo

Gaiero

Carro

et al. 2016

Plant Tissue Cult. & Biotech.

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The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L-cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.

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“…Haploids have a significant role in potato breeding programs of quite a few companies, since they enable interspecific hybridization, which would not otherwise be possible due to differences in ploidy levels and endosperm balance numbers. The gene pool of potato can be broadened and certain valuable traits, such as disease resistance characters from the wild solanaceous species, can be more efficiently introgressed into cultivated potato (Rokka, 2009). …”

Section: Wide Hybridizationmentioning

confidence: 99%