Potato cultivar identification using simple sequence repeats markers (SSR)

Moisan-Thiery, M.; Marhadour, Sylvie; Kerlan, M. C.; Dessenne, N.; Perramant, M.; Gokelaere, T.; Hingrat, Yves

doi:10.1007/bf02742376

Cited by 46 publications

(34 citation statements)

References 25 publications

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“…Fresh tender leaves (~100 mg) from the plantlets were used to extract DNA according to the CTAB procedure of Moisan-Thiery et al (2005). DNA quantity and quality were assessed on 0.8% (w/v) agarose gel with lambda DNA as concentration standard.…”

Section: Dna Extractionmentioning

confidence: 99%

“…The origins of the potato cultivars must be taken into consideration, however. The selection of SSR primers ought to be based on their origin, since with the increase in the number of potato cultivar entries there is a risk of identical molecular patterns appearing for distinct cultivars (Moisan-Thiery et al, 2005). In China itself the origin of potato cultivars is debatable due to the lack of extensive records about the introduction of potato in China.…”

Section: The Potential Of Ssr Technique For Construction Of Core Collmentioning

confidence: 99%

“…Other SSR sites have been found in the potato genome since then, including highly informative and user-friendly microsatellites (Ghislain et al, 2004). SSR markers have been used to identify French potato cultivars (Moisan-Thiery et al, 2005), discriminate potato germplasm in the INIA Chile breeding program (Mathias et al, 2007), develop a rapid SSR-based identification method for potato cultivars (Reid and Kerr, 2007), develop an SSR-based genetic identity kit for potato (Ghislain et al, 2009), and discriminate genetic relationships in Spanish potato cultivars (de Galarreta et al, 2011). This work has provided insights useful for germplasm management and discrimination of potato cultivars.…”

Section: Introductionmentioning

confidence: 99%

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Using SSR to Evaluate the Genetic Diversity of Potato Cultivars from Yunnan Province (SW China)

Liao¹,

Guo²

2014

Acta Biologica Cracoviensia S. Botanica

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The genetic diversity of potato cultivars collected from Yunnan Province was evaluated using 24 pairs of SSR markers. SSR analysis of 24 pairs of primers showed varying degrees of polymorphism among the 85 cultivars: 297 of the 304 bands were polymorphic. The primers yielded between 5 (STM2028) and 19 (StI029) bands (mean 12). The ratio of polymorphic bands ranged from 83.33% to 100% (mean 97.75%). Polymorphism information content (PIC) varied from 69.31% to 93.67% (mean 86.47%). Genetic similarity ranged from 0.5987 to 0.7632, indicating relatively low genetic diversity in the potato cultivars from Yunnan Province. Cluster analysis by UPGMA and PCA clearly delineated the genetic relationships of all cultivars; 83 of the 85 cultivars could be discriminated by only two pairs of primers, STM0030 and STM1104. The high polymorphism and good resolution of the primers used in this study make them good tools for discriminating potato cultivars.K Ke ey y w wo or rd ds s: : Solanum tuberosum L., SSR markers, genetic diversity, potato cultivars.

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Section: Dna Extractionmentioning

confidence: 99%

Section: The Potential Of Ssr Technique For Construction Of Core Collmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Using SSR to Evaluate the Genetic Diversity of Potato Cultivars from Yunnan Province (SW China)

Liao¹,

Guo²

2014

Acta Biologica Cracoviensia S. Botanica

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“…Simple sequence repeats (SSRs, or microsatellites) have been used to great advantage in potato for studies of diversity, genetic structure, and classification ); tracing germplasm migrations (Spooner et al 2005a;Rios et al 2007); fingerprinting (Moisan-Thiery et al 2005;Provan et al 1996;Schneider and Douches 1997); genetic linkage mapping (Ghislain et al 2001;Feingold et al 2005); establishment of core collections (Ghislain et al 2006); and investigations of duplicate collections across genebanks (Del Rio et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Robust and highly informative microsatellite-based genetic identity kit for potato

et al. 2008

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The fingerprinting of 742 potato landraces with 51 simple sequence repeat (SSR, or microsatellite) markers resulted in improving a previously constructed potato genetic identity kit. All SSR marker loci were assayed with a collection of highly diverse landraces of all species of cultivated potato with ploidies ranging from diploid to pentaploid. Loci number, amplification reproducibility, and polymorphic information content were recorded. Out of 148 SSR markers of which 30 are new, we identified 58 new SSR marker locations on at least one of three potato genetic linkage maps. These results permitted the selection of a new potato genetic identity kit based on 24 SSR markers with two per chromosome separated by at least 10 cM, single locus, high polymorphic information content, and high quality of amplicons as determined by clarity and reproducibility. The comparison of a similarity matrix of 742 landraces obtained with the 24 SSR markers of the new kit and with the entire dataset of 51 SSR markers showed a high correlation (r = 0.94) by a Mantel test and even higher correlations (r = 0.99) regarding topological comparisons of major branches of a neighbor joining tree. This new potato genetic identity kit is able to discriminate 93.5% of the 742 landraces compared to 98.8% with 51 SSR markers. In addition, we made a marker-specific set of allele size standards that conveniently and unambiguously provide accurate sizing of all alleles of the 24 SSR markers across laboratories and platforms. The new potato genetic identity kit will be of particular utility to standardize the choice and allele sizing of microsatellites in potato and aid in collaborative projects by allowing cumulative analysis of independently generated data.

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“…Важное значение для повышения эффективности се лекции имеют также генотипирование сортов (Feingold et al, 2005;MoisanThiery et al, 2005) и реконструкция родословных селекционных сортов (Braun, Wenzel, 2004). Для генотипирования сортов картофеля сейчас широко применяются ДНКмаркеры, основанные на полимераз ной цепной реакции: RAPD, ISSR, AFLP, SSR (Hosaka, Ogawa, 1994;Brаun, Wenzel, 2004;MoisanThiery et al, 2005; Бирюкова и др., 2006), из которых наиболее эффек тивны микросателлитные или SSRмаркеры.…”

Section: использование генетического разнообразия картофеля в совремеunclassified

Mobilization, conservation and study of cultivated and wild potato genetic resources

Kiru¹,

Рогозина²

2017

Vestn. VOGiS

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Potato cultivar identification using simple sequence repeats markers (SSR)

Cited by 46 publications

References 25 publications

Using SSR to Evaluate the Genetic Diversity of Potato Cultivars from Yunnan Province (SW China)

Using SSR to Evaluate the Genetic Diversity of Potato Cultivars from Yunnan Province (SW China)

Robust and highly informative microsatellite-based genetic identity kit for potato

Mobilization, conservation and study of cultivated and wild potato genetic resources

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