Potato apyrase reduces granulomatous area and increases presence of multinucleated giant cells in murine schistosomiasis

Gusmão, Michélia A.N.; Júnior, Sérgio Mendanha; Marconato, Danielle Gomes; Emídio, Nayara Braga; Farani, Priscila Silva Grijó; Gollner, Ângela Marial; Araújo, Neusa; Coelho, Paulo Marcos Zech; Macedo, Gilson Costa; Filho, Ademar Alves da Silva; Vasconcelos, Eveline Gomes; Faria-Pinto, Priscila

doi:10.1016/j.parint.2021.102317

Cited by 5 publications

(7 citation statements)

References 30 publications

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“…Taken together, molecular docking studies obtained in this work corroborate the action of cardamonin in binding and inhibiting apyrase and SmNTPDases. Moreover, previous studies also showed a similarity of the active sites between potato apyrase and S. mansoni ATP NTPDases isoforms, as well as that potato apyrase and S. mansoni NTPDases have cross‐immunoreactivity, indicating that these proteins share epitopes, including those that recognize antibody production [18–20,26] . Additionally, the homology of potato apyrase to S. mansoni NTPDases isoforms could make possible to use potato apyrase in the initial screening for searching new schistosomicidal NTPDases inhibitors [19] .…”

Section: Resultsmentioning

confidence: 91%

“…Regarding its mechanism of action, the schistosomicidal activity of cardamonin is related to its ability in inhibiting the SmNTPDases [9] . In this regard, some studies have demonstrated the homology between potato apyrase and SmNTPDases [15,17–20] and that some schistosomicidal compounds were able to promote the inhibition of the enzymatic activity of both potato apyrase and S. mansoni NTPDases [19,26] . Concerning this, we hypothesized if cardamonin may also act on potato apyrase.…”

Section: Resultsmentioning

confidence: 97%

“…In addition, previous studies showed a similarity in the active sites of SmNTPDases and potato ( Solanum tuberosum ) apyrase [15,17,18] . In fact, potato apyrase and SmNTPDases have cross‐immunoreactivity, suggesting that both proteins share epitopes, including those that recognize antibody production [15,17–20] . Moreover, the homology between potato apyrase and SmNTPDases isoforms might make possible to use potato apyrase in the initial screening for searching new schistosomicidal SmNTPDases inhibitors [19] .…”

Section: Introductionmentioning

confidence: 98%

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Cardamonin Presents in Vivo Activity against Schistosoma mansoni and Inhibits Potato Apyrase

Carvalho

Silva

Souza

et al. 2021

Chemistry & Biodiversity

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Schistosomiasis, a neglected tropical disease caused by Schistosoma species, harms over 250 million people in several countries. The treatment is achieved with only one drug, praziquantel. Cardamonin, a natural chalcone with in vitro schistosomicidal activity, has not been in vivo evaluated against Schistosoma. In this work, we evaluated the in vivo schistosomicidal activities of cardamonin against Schistosoma mansoni worms and conducted enzymatic apyrase inhibition assay, as well as molecular docking analysis of cardamonin against potato apyrase, S. mansoni NTPDase 1 and S. mansoni NTPDase 2. In a mouse model of schistosomiasis, the oral treatment with cardamonin (400 mg/kg) showed efficacy against S. mansoni, decreasing the total worm load in 46.8 % and reducing in 54.5 % the number of eggs in mice. Cardamonin achieved a significant inhibition of the apyrase activity and the three‐dimensional structure of the potato apyrase, obtained by homology modeling, showed that cardamonin may interact mainly through hydrogen bonds. Molecular docking studies corroborate with the action of cardamonin in binding and inhibiting both potato apyrase and S. mansoni NTPDases.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

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Cardamonin Presents in Vivo Activity against Schistosoma mansoni and Inhibits Potato Apyrase

Carvalho

Silva

Souza

et al. 2021

Chemistry & Biodiversity

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“…Schistosoma eggs found in the host tissues trigger an inflammatory response known as granulomatous, characterized by the presence of granulomas in the infected tissues ( Farah et al., 2000 ). Gusmão et al. (2021) showed that immunization of C57BL/6 mice with potato apyrase was ineffective in triggering a protective immune response against Schistosoma infection.…”

Section: Development Of E-ntpdases Inhibitorsmentioning

confidence: 99%

“…Differently, immunization using potato apyrase decreased the granulomatous response, accompanied by an enhancement of multinucleated giant cells. In this sense, immunotherapies developed from potato apyrase-immunization to avoid granuloma development seems to be a potential prophylaxis strategy ( Gusmão et al., 2021 ).…”

Section: Development Of E-ntpdases Inhibitorsmentioning

confidence: 99%

E-NTPDases: Possible Roles on Host-Parasite Interactions and Therapeutic Opportunities

Paes-Vieira

Gomes-Vieira

Meyer‐Fernandes

2021

Front. Cell. Infect. Microbiol.

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Belonging to the GDA1/CD39 protein superfamily, nucleoside triphosphate diphosphohydrolases (NTPDases) catalyze the hydrolysis of ATP and ADP to the monophosphate form (AMP) and inorganic phosphate (Pi). Several NTPDase isoforms have been described in different cells, from pathogenic organisms to animals and plants. Biochemical characterization of nucleotidases/NTPDases has revealed the existence of isoforms with different specificities regarding divalent cations (such as calcium and magnesium) and substrates. In mammals, NTPDases have been implicated in the regulation of thrombosis and inflammation. In parasites, such as Trichomonas vaginalis, Trypanosoma spp., Leishmania spp., Schistosoma spp. and Toxoplasma gondii, NTPDases were found on the surface of the cell, and important processes like growth, infectivity, and virulence seem to depend on their activity. For instance, experimental evidence has indicated that parasite NTPDases can regulate the levels of ATP and Adenosine (Ado) of the host cell, leading to the modulation of the host immune response. In this work, we provide a comprehensive review showing the involvement of the nucleotidases/NTPDases in parasites infectivity and virulence, and how inhibition of NTPDases contributes to parasite clearance and the development of new antiparasitic drugs.

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Endophytic Fungi: Potential Source of Allelochemicals for Sustainable Agriculture

Zafar,

Afzal,

Ijaz

et al. 2024

Sustainable Plant Nutrition in a Changing World

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Potato apyrase reduces granulomatous area and increases presence of multinucleated giant cells in murine schistosomiasis

Cited by 5 publications

References 30 publications

Cardamonin Presents in Vivo Activity against Schistosoma mansoni and Inhibits Potato Apyrase

Cardamonin Presents in Vivo Activity against Schistosoma mansoni and Inhibits Potato Apyrase

E-NTPDases: Possible Roles on Host-Parasite Interactions and Therapeutic Opportunities

Endophytic Fungi: Potential Source of Allelochemicals for Sustainable Agriculture

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