Potassium sorbate as an AGE activator for human serum albumin in the presence and absence of glucose

Taghavi, Fereshteh; Moosavi-Movahedi, Ali Akbar; Bohlooli, Mousa; Hadi-Alijanvand, Hamid; Salami, Maryam; Maghami, Parvaneh; Saboury, Ali Akbar; Farhadi, Mohammad; Yousefi, Reza; Habibi-Rezaei, Mehran

doi:10.1016/j.ijbiomac.2013.08.045

Cited by 19 publications

(18 citation statements)

References 42 publications

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“…PS is the most applicable material in food and pharmaceutical industries. The conformational changes of HSA due to glucose and PS were reported in our previous studies …”

Section: Introductionsupporting

confidence: 60%

“…We previously reported the grown size of HSA particles in HSA + Glc 120D sample (105.5 nm) without any fibril formation, spherulite structure in HSA + PS 120D, and high density of long amyloid fibrils in HSA + PS + Glc 120D . The direct observation through AFM (Figure , panel c) showed no particle in HSA + Glc + El, two shapes in HSA + PS + El samples: spherulites with changed shape from its control (HSA + PS) with a diameter of 89 nm (Figure , panel a) compared with the spherulite in HSA + PS (1.457 μm × 2.164 μm) and some light aggregation (892.7 nm × 1.277 nm) (Figure , panel b). These results are completely in agreement with the results of the Th T assay (Figure ).…”

Section: Discussionmentioning

confidence: 86%

“…Protein glycooxidation takes place directly by glucose and indirectly by some kind other oxidative agents . In previous research we demonstrated that PS as a highly potent preservative was able to form different HSA intermediates with different conformational shapes (fibril and spherulites) due to 120‐day incubation in the presence and absence of glucose . Phenolic antioxidants have a powerful effect on oxidative stress inhibition and consequent diseases .…”

Section: Discussionmentioning

confidence: 99%

“…This site is located in site I within HSA subdomain IIA. Hydrophobic interaction and hydrogen bonds are involved forces in this connection, while PS interact with HSA through two different location: (1) free lysine residues in the surface of HSA through covalent bonds and (1) a possible binding site between subdomains IA and IB of HSA via hydrogen and hydrophobic interactions . So it seems that there are no competition between PS and EL to interact with HSA.…”

Section: Discussionmentioning

confidence: 99%

“…The conformational changes of HSA due to glucose and PS were reported in our previous studies. 37,38 In this study the amyloid inhibition effect of ellagic acid and its mechanism on HSA fibrillation due to PS and glucose treatment were investigated.…”

Section: Introductionmentioning

confidence: 99%

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Antiamyloidogenic Effects of Ellagic Acid on Human Serum Albumin Fibril Formation Induced by Potassium Sorbate and Glucose

Taghavi

Habibi-Rezaei

Bohlooli

et al. 2016

J of Molecular Recognition

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Oxidative stress has the main role in protein conformational changes and consequent direct involvement in different kind of diseases. Potassium sorbate as a widespread industrial preservative and glucose are two important oxidants that can be involved in oxidative stress. In this study the effect of ellagic acid as a phenolic antioxidant on amyloid fibril formation of human serum albumin upon incubation of potassium sorbate and glucose was studied using thioflavin T assay, surface tension, atomic force microscopy, Amadori product, and carbonyl content assays. The thioflavin T assay and atomic force microscopy micrographs demonstrated the antiamyloidogenic effect of ellagic acid on the human serum albumin fibril formation. This antioxidant also had the repair effect on surface tension of the modified human serum albumin (amyloid intermediates), which was destructed, caused by potassium sorbate and glucose. This mechanism takes place because of potent carbonyl stress suppression effect of ellagic acid, which was strengthening by potassium sorbate in the presence and absence of glucose.

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“…PS is the most applicable material in food and pharmaceutical industries. The conformational changes of HSA due to glucose and PS were reported in our previous studies …”

Section: Introductionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations