Potassium Phosphite Enhances the Antagonistic Capability of Bacillus amyloliquefaciens to Manage Tomato Bacterial Wilt

Abstract

Bacterial wilt caused by Ralstonia solanacearum is a distributed and worldwide soil-borne disease. The application of biocontrol microbes or agricultural chemicals has been widely used to manage tomato bacterial wilt. However, whether and how agricultural chemicals affect the antagonistic ability of biocontrol microbes is still unknown. Here, we combined potassium phosphite (K-Phite), an environmentally friendly agricultural chemical, and the biocontrol agent Bacillus amyloliquefaciens QPF8 (strain F8) to manage tomato bacterial wilt disease. First, K-Phite at a concentration of 0.05% (w/v) could significantly inhibit the growth of Ralstonia solanacearum. Second, 0.05% K-Phite enhanced the antagonistic capability of B. amyloliquefaciens F8. Third, the greenhouse soil experiments showed that the control efficiency for tomato bacterial wilt in the combined treatment was significantly higher than that of the application of B. amyloliquefaciens F8 or K-Phite alone. Overall, our results highlighted a novel strategy for the control of tomato bacterial wilt disease via application and revealed a new integrated pattern depending on the enhancement of the antagonistic capability of biocontrol microbes by K-Phite.

Changes to the Bacterial Microbiome in the Rhizosphere and Root Endosphere of Persea americana (Avocado) Treated With Organic Mulch and a Silicate-Based Mulch or Phosphite, and Infested With Phytophthora cinnamomi

“…Phosphite is widely used to control P. cinnamomi damage in avocado orchards, and there is little data on how this might affect the soil microbiome. In tomato crops, phosphite has a beneficial effect; it suppresses Ralstonia solanacearum , and, when applied in conjunction with the biocontrol agent Bacillus amyloliquefaciens, it enhances its antagonistic activity (Su et al, 2021b ).…”

Integrated biological and chemical control of Fusarium wilt by Bacillus velezensis EB1 and potassium sorbate

“…Moreover, the widespread use of fungicides has been increasingly limited because of the environmental risk and the emergence of fungicide-resistant strains of fungal pathogens [24]. Therefore, many studies have focused on combinations of fungicides and BCAs to effectively inhibit pathogens [15,[25][26][27]. However, few studies have shown the integrated management of banana Fusarium wilt by treatment with BCAs and fungicides.…”

“…Zakaria et al (2023) showed that the application of CBZ + SS + BS (a combination of the fungicide carbendazim, sodium silicate, and Bacillus subtilis) in farms signi cantly reduced the disease incidence by 16.07% and disease severity by 14.28% and enhanced the morphophysiological growth performance, with an average production yield of approximately 24.72 kg per fruit bunch [14]. The control e ciency of combined application of potassium phosphite with the antagonist bacterium B. amyloliquefaciens against tomato bacterial wilt was signi cantly greater than that of B. amyloliquefaciens F8 or K-Phite alone [15].…”