Potassium monopersulfate and a water-soluble manganese porphyrin complex, [Mn(TMPyP)](OAc)5, as an efficient reagent for the oxidative cleavage of DNA

Abstract

Reported studies indicate that the association of potassium monopersulfate with [Mn(TMPyP)](OAc)5, a water-soluble manganese porphyrin complex, leads to an efficient reagent for the oxidative cleavage of DNA. Single-strand breaks (SSBs) are observed on double-stranded DNA at manganese porphyrin concentrations as low as 0.5 nM with a short incubation time of 1 min. The number of SSBs linearly varies with the concentration of the manganese complex, and potassium monopersulfate is at least 3 orders of magnitude more efficient as oxygen source than hydrogen peroxide. Cleavage efficiency is optimal in the pH range 7.5-9.0 for a NaCl concentration between 80 and 150 mM or for a MgCl2 concentration of 10 mM. At very low manganese porphyrin concentration and by increasing the incubation time a catalytic cleavage activity of the complex is evidenced: up to 5 SSBs per manganese porphyrin are observed. The high cleavage activity of the monopersulfate-manganese porphyrin system makes it a good candidate for DNA-footprinting experiments.

Structures, spectra, and DNA-binding properties of mixed ligand copper(II) complexes of iminodiacetic acid: The novel role of diimine co-ligands on DNA conformation and hydrolytic and oxidative double strand DNA cleavage

“…After electrophoresis the proportion of DNA in each fraction was estimated quantitatively from the intensities of the bands using UVP Bioimaging -GDS-8000 Gel Documentation System using the Labwork software. The fraction of the original supercoiled DNA converted to the nicked and linear form at the end of the reaction was calculated after correcting for the low level of NC present in the original sample and the low affinity of ethidium bromide binding to SC compared to nicked and linear forms of DNA [36]. Inhibition reactions were carried out by prior incubation of the SC pBR322 DNA (10 lM) with ethanol (1 M), sodium azide (100 mM), superoxide dismutase (10 U) and distamycin (100 lM).…”

Electronic Structure and Spectro-Structural Correlations of FeIIIZnII Biomimetics for Purple Acid Phosphatases: Relevance to DNA Cleavage and Cytotoxic Activity

“…The proportion of plasmid DNA in each band was quantified using KODAK Molecular Imaging Software 5.0 (Carestream Health, U.S.A.). The portion of supercoiled DNA (F I) was corrected by a factor of 1.47, 45 since the ability of ethidium bromide to intercalate into this DNA topoisomeric form is decreased relative to open circular and linear DNA. To elucidate whether the mechanism of DNA cleavage performed by complexes 1-4 is hydrolytic or oxidative, different inhibitors of reactive oxygen species (ROS) were added to the DNA treatments prior to the complexes.…”

Crystal structure, supramolecular self-assembly and interaction with DNA of a mixed ligand manganese(II) complex

“…17,18 Some manganese complexes, e.g. [Mn(TMPyP)](OAc) 5 (TMPyP = meso-tetrakis(4-N-methylpyridinumyl) porphyrinato), 19 manganese-salen derivatives, 20,21 [Mn(L) 23 also present intercalative DNA-binding capacity or significant DNA/antitumor activity. Therefore the synthesis and characterization of new manganese complexes are of great importance not only for developing novel chemotherapeutics and highly sensitive diagnostic agents but also for understanding the role and mechanism of manganese complexes in biological system.…”