Potassium metabisulphite as a potential biocide against <i>Dekkera bruxellensis</i>
 in fuel ethanol fermentations

Bassi, Ana Karolina Zamprônio; Paraluppi, Anna Livia; Reis, Vanda Renata; Ceccato-Antonini, Sandra Regina

doi:10.1111/lam.12363

Cited by 3 publications

(3 citation statements)

References 22 publications

(49 reference statements)

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“…This result may be attributed to the yeast composition of the juice, which is known to vary along the sugarcane maturation. Saccharomyces yeasts are more resistant to PMB than non-Saccharomyces yeasts (Divol et al, 2012;Bassi, Paraluppi, Reis, & Ceccato-Antonini, 2015) and the proportion between Saccharomyces and non-Saccharomyces in the sugarcane juice oscillates according to the sugarcane maturation (Martini, Margarido, & Ceccato-Antonini, 2010).The sugarcane juice has a diversity of bacteria and fungi that can compete with the starter yeast S. cerevisiae and affect the fermentative yield. Genera as Candida, Debaryomyces, Hanseniaspora, Meyerozyma, Wickerhamiella and Zygosaccharomyces were observed to constitute 11.9% of the yeasts present in the sugarcane stalks, and Candida represents the major fraction (Souza et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

High doses of potassium metabisulphite are required to control the growth of native bacteria and yeasts from sugarcane juice

Paraluppi

Ceccato-Antonini

2019

Acta Sci. Technol.

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A major concern of ethanol production plants is the control of native microorganisms mainly derived from the sugarcane juice that may reduce the fermentative yield. In the wine industry, the sulphur dioxide (SO2) is commonly used to control the undesirable populations of yeast and bacteria, however, this substance has not been evaluated yet in the context of the ethanol industry. This study aimed to verify the effect of different concentrations and incubation times of potassium metabisulphite (PMB), as a source of SO2, with raw sugarcane juice in order to control the growth of native bacteria and yeasts. PMB was effective to control the growth of native yeasts and bacteria from the sugarcane juice at the concentration of 800 mg L -1 , reducing almost one log cycle for yeasts and 1.9-2.9 log cycles for bacteria, with incubation times of 3 and 6-9 hours, respectively. Although PMB is effective in the context of wine fermentation, it was not appropriate to the fuel ethanol production especially due to the peculiar characteristics of the substrate and also to the cost in terms of high doses of the antimicrobial and the volumes of sugarcane juice to be treated daily.

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Section: Resultsmentioning

confidence: 99%

High doses of potassium metabisulphite are required to control the growth of native bacteria and yeasts from sugarcane juice

Paraluppi

Ceccato-Antonini

2019

Acta Sci. Technol.

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“…In addition, acid cell washing is occasionally inefficient, and the corrosive acids are a serious safety risk. Strategies to control the growth of D. bruxellensis in the bioethanol industry are scarce (BASSI et al, 2013(BASSI et al, , 2014. Thus, new antimicrobials should be researched and tested.…”

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confidence: 99%

“…Two hydroalcoholic extracts of propolis were selected for the quantitative in vitro assay, which was performed according to Bassi et al (2014) with some modifications. Briefly, 50 mL Falcon tubes containing a final volume of 10 mL were prepared with 7.5 mL of YPD broth, 1 mL of yeast suspension (as described above) and 0.75 or 1.5 mL of each hydroalcoholic extract of propolis (propolis final concentration of 4.5 and 9 mg mL -1 ).…”

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confidence: 99%

Propolis as a potential alternative for the control of Dekkera bruxellensis in bioethanol fermentation

Fernández

Cibanal

Paraluppi

et al. 2019

SCA

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Dekkera bruxellensis is one of the most important contaminant yeasts of alcoholic fermentation. The use of propolis, which can selectively target contaminating yeasts without affecting the starter one, Saccharomyces cerevisiae, could be a useful nonconventional strategy for controlling the growth of contaminant yeasts. The objective of this research was to evaluate four samples of propolis produced by Apis mellifera honeybees from different regions of Argentina as antimicrobial agents against the growth of D. bruxellensis and S. cerevisiae. Hydroalcoholic extracts of propolis were prepared with ethanol:water (70:30 v/v), and the specific absorbance and final concentration of the samples were evaluated. A qualitative in vitro assay in solid medium was performed with different propolis concentrations, and the evaluation of yeast growth was based on a qualitative scale. A quantitative in vitro assay in liquid medium was also performed to assess the yeast cell number, using two different propolis concentrations. The cell number of D. bruxellensis decreased 1.52 and 1.85 log cycles with the two propolis extracts utilised at a concentration of 4.5 mg mL-1 while the cell number of S. cerevisiae decreased 0.48 and 0.76 log cycles with the same samples of propolis. The results from both assays demonstrated the selectivity of propolis use on the yeast species, leading to a higher inhibition of D. bruxellensis growth. This indicates a good potential for using propolis at the concentration of 4.5 mg mL-1, as a nonconventional strategy to control the growth of D. bruxellensis without significantly affecting S. cerevisiae, the yeast starter of ethanol fermentation.

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Native Yeasts and Their Role in Ethanol Fermentation

Ceccato-Antonini

2022

Microbiology of Ethanol Fermentation in Sugarcane Biofuels

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Potassium metabisulphite as a potential biocide against Dekkera bruxellensis in fuel ethanol fermentations

Cited by 3 publications

References 22 publications

High doses of potassium metabisulphite are required to control the growth of native bacteria and yeasts from sugarcane juice

High doses of potassium metabisulphite are required to control the growth of native bacteria and yeasts from sugarcane juice

Propolis as a potential alternative for the control of Dekkera bruxellensis in bioethanol fermentation

Native Yeasts and Their Role in Ethanol Fermentation

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