Potassium Channel Conductance: A Mechanism Affecting Hair Growth both In Vitro and In Vivo

Buhl, Allen E.; Waldon, Daniel J.; Conrad, Steven J.; Mulholland, Michael J; Shull, Kathy L.; Kubicek, Marc F.; Johnson, George; Brunden, Marshall N.; Stefanski, Kevin; Stehle, Randall G.; Gadwood, Robert C.; Kamdar, Bharat V.; Thomasco, Lisa M.; Schostarez, Heinrich J.; Schwartz, Theresa; Diani, A. R.

doi:10.1111/1523-1747.ep12499788

Cited by 81 publications

(47 citation statements)

References 24 publications

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“…35 S]-cysteine uptake is correlated to hair follicle growth in vitro (15), these results suggest that adenosine produces a stimulating effect on hair growth.…”

Section: Adenosine Activates and Elongates The Anagen Phase Of Mouse mentioning

confidence: 65%

Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by increasing the cysteine level via fibroblast growth factors 2 and 7 in an organ culture of mouse vibrissae hair follicles

Hwang

Kim²,

Roh³

et al. 2011

Int J Mol Med

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Abstract. Hair regression and balding are distressing concerns for an increasing number of people due to changes in lifestyle and serious nutritional imbalances. Therapies for treatment of hair loss are needed. Among potential therapeutics, adenosine has been suggested as a potent regulator of hair growth. In this study, we investigated the effects of adenosine on hair follicles and dermal papilla (DP) cells, and the mechanism underlying the action of adenosine. Hair follicles are organs, including DP cells, that are responsible for the production of hair fibers by inducing and maintaining the hair growth phase (anagen). In a culture of DP cells in vitro, adenosine stimulated proliferation of DP cells by increasing thymidine uptake. Subsequently, adenosine activated and elongated the anagen phase by increasing the uptake of radiolabeled cysteine in an organ culture of mouse vibrissae hair follicles. We also confirmed that adenosine promoted the expression of several growth factors that are responsible for hair growth, including fibroblast growth factors (FGF)-7, FGF-2, insulin-like growth factor (IGF)-1, and vascular endothelial growth factor (VEGF) in a cDNA microarray with semi-quantitative RT-PCR. Transcriptional activation of β-catenin in DP cells was increased by adenosine in a luciferase assay. β-catenin is a co-activator of Wnt/β-catenin signaling that induces morphogenesis and differentiation of hair follicles and also acts to transactivate downstream signaling pathways, including the ERK pathway. Using Western blotting, we found that adenosine stimulated phosphorylation of ERK, CREB and AKT. These results suggest that adenosine stimulates growth of hair follicles by triggering the expression of growth factors and β-catenin, and by inducing their downstream target signaling pathways.

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“…35 S]-cysteine uptake is correlated to hair follicle growth in vitro (15), these results suggest that adenosine produces a stimulating effect on hair growth.…”

Section: Adenosine Activates and Elongates The Anagen Phase Of Mouse mentioning

confidence: 65%

Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by increasing the cysteine level via fibroblast growth factors 2 and 7 in an organ culture of mouse vibrissae hair follicles

Hwang

Kim²,

Roh³

et al. 2011

Int J Mol Med

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“…Although minoxidil has vasodilator function, when used systemically for hypertension, its mechanism of action in hair loss involves a direct stimulatory effect on dermal papilla or follicular hair matrix cell, increasing m-RNA expres sion for VEGF, and inducing angiogenesis (15). Recently, it was supposed that minoxidil might activate the prostaglandine-endoperossidosynthetase enzyme type 1 which stimulates hair growth (16,17). Minoxidil appears to act by altering the hair cycle, through a combination of two effects, either shortening the telogen or prolonging the anagen phases (11).…”

Section: 'L It I N I' ' ' 'mentioning

confidence: 99%

“…Minoxidil appears to act by altering the hair cycle, through a combination of two effects, either shortening the telogen or prolonging the anagen phases (11). Moreover, this drug, inhibiting the enzyme Iysyl hydroxylase activity in human skin fibroblast cultures , leads to synthesi s of a collagen poor in hydroxylysine (18), developing antifibrotic activity (17). Therefore, this drug is useful in preventing the atrophy that can be observed in this disorder.…”

Section: 'L It I N I' ' ' 'mentioning

confidence: 99%

Use of Topical Minoxidil, 17α-Estradiol and Hydrocortisone Butyrate in Frontal Fibrosing Alopecia

Rossi

Iorio

Scarnò³

et al. 2014

Eur J Inflamm

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A valid approach to treating frontal fibrosing alopecia (FFA), a scarring alopecia, does not yet exist. The aim of this study is to evaluate the efficacy of a topical application composed of minoxidil, hydrocortisone butyrate, 17a-estradiol, ciclosilicone pentamer, and alcohol in women affected by FFA. Forty women affected by FFA were treated with 2 ml of a topical lotion composed of 2% minoxidil base, 0.08% hydrocortisone butyrate, 0.05% 17a-estradiol, 16% ciclosilicone pentamer, 96°alcohol, applied once a day on the scalp. A score index, based on the comparison of the photos, taken at the beginning (TO) and after 36 months (T36), was used. The topical lotion led to an improvement in 20 patients, stabilization in 15 patients and worsening of the clinical condition in 5 women. This topical lotion induces a considerable improvement or stabilization of frontal fibrosing alopecia.

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“…Uptake and conversion of minoxidil to minoxidil sulfate occurs within the HF, suggesting a direct action on the follicle [134]. The most probable site of action of minoxidil is the DP [135], and the mechanism of action has been linked to its effects on the Kir6.1/SUR2B potassium channel expressed by the derma papilla [136][137][138].…”

Section: Treatments Of Hair Growth Disorders: Room For Improvementmentioning

confidence: 99%

Biology of Human Hair: Know Your Hair to Control It

Araújo

Fernandes

Cavaco‐Paulo

et al. 2010

Advances in Biochemical Engineering/Biotechnology

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Hair can be engineered at different levels-its structure and surfacethrough modification of its constituent molecules, in particular proteins, but also the hair follicle (HF) can be genetically altered, in particular with the advent of siRNA-based applications. General aspects of hair biology are reviewed, as well as the most recent contributions to understanding hair pigmentation and the regulation of hair development. Focus will also be placed on the techniques developed specifically for delivering compounds of varying chemical nature to the HF, indicating methods for genetic/biochemical modulation of HF components for the treatment of hair diseases. Finally, hair fiber structure and chemical characteristics will be discussed as targets for keratin surface functionalization.

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Potassium Channel Conductance: A Mechanism Affecting Hair Growth both In Vitro and In Vivo

Cited by 81 publications

References 24 publications

Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by increasing the cysteine level via fibroblast growth factors 2 and 7 in an organ culture of mouse vibrissae hair follicles

Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by increasing the cysteine level via fibroblast growth factors 2 and 7 in an organ culture of mouse vibrissae hair follicles

Use of Topical Minoxidil, 17α-Estradiol and Hydrocortisone Butyrate in Frontal Fibrosing Alopecia

Biology of Human Hair: Know Your Hair to Control It

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