Potassium but Not Lactate Dehydrogenase Elevation Due To In Vitro Hemolysis Is Higher in Capillary Than in Venous Blood Samples

Oostendorp, M. van; Solinge, Wouter W. van; Kemperman, Hans

doi:10.5858/arpa.2011-0319-oa

Cited by 11 publications

(5 citation statements)

References 20 publications

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“…29 The elevation of potassium due to hemolysis has been reported to be more important in capillary blood samples compared to venous blood samples, which is of special interest to POCT applications. 32 It was also shown that temperature during transport might lead to misdiagnosis and "pseudo hyperkalemia", or so-called "seasonal pseudohyperkalemia". 33 To alleviate this issue, a study showed that the introduction of centrifuges in UK GP surgeries led to a reduction of these cases, thereby proving the benefits of rapid, point-of-care plasma extraction.…”

Section: Influence Of Hemolysis In Blood Samplesmentioning

confidence: 99%

Microfluidic blood plasma separation for medical diagnostics: is it worth it?

et al. 2016

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Circulating biomarkers are on the verge of becoming powerful diagnostic tools for various human diseases. However, the complex sample composition makes it difficult to detect biomarkers directly from blood at the bench or at the point-of-care. Blood cells are often a source of variability of the biomarker signal. While the interference of hemoglobin is a long known source of variability, the release of nucleic acids and other cellular components from hemocytes is a new concern for measurement and detection of circulating extracellular markers. Research into miniaturised blood plasma separation has been thriving in the last 10 years (2006-2016). Most point-of-care systems need microscale blood plasma separation, but developed solutions differ in complexity and sample volume range. But could blood plasma separation be avoided completely? This focused review weights the advantages and limits of miniaturised blood plasma separation and highlights the most interesting advances in direct capture as well as smart blood plasma separation.

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Section: Influence Of Hemolysis In Blood Samplesmentioning

confidence: 99%

Microfluidic blood plasma separation for medical diagnostics: is it worth it?

et al. 2016

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“…Of course, we considered the possibility that high LDH level might be due to difficulty experienced during collection or handling of the sample. Because the other laboratory results were all normal, and careful monitoring was taken, we could rule out the errors [15,16]. …”

Section: Discussionmentioning

confidence: 99%

Elevation of serum lactate dehydrogenase in patients with pectus excavatum

Kim

Park

et al. 2014

J Cardiothorac Surg

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IntroductionPectus excavatum is the most common congenital chest wall deformity and the depression of the anterior chest wall, which compresses the internal organs. The aim of the present study is to investigate the effects of pectus excavatum on blood laboratory findings.Material and MethodsFrom March 2011 to December 2011, 71 patients with pectus excavatum who visited Seoul Saint Mary Hospital for Nuss procedure were reviewed and analyzed. The blood samples were routinely taken at the day before surgery and pectus bar removal was usually performed in 2 to 3 years after Nuss procedure. To investigate the effects on blood laboratory findings, preoperative routine blood laboratory data and postoperative changes of abnormal laboratory data were analyzed.ResultsOnly lactate dehydrogenase (LDH), one of 26 separate routine laboratory tests, was abnormal and significantly elevated than normal value (age <10, p = 0.008; age ≥10, p < 0.001). However, there was no significant correlation between LDH levels and severities of pectus excavatum. The symmetric subgroup had significantly higher LDH level than the asymmetric subgroup (p <0.001) and there was a significant decrease of LDH level after correction of deformity (p = 0.017).ConclusionIn conclusion, only LDH, one of the routine laboratory tests, was significantly elevated than normal value, which was thought to be caused by etiologies of pectus excavatum and the compression of the internal organs. Further studies on LDH including isoenzyme studies in patients with pectus excavatum will be needed, and these studies will provide a deeper and wider comprehension of pectus excavatum.

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“…For instance, differences in the effect of haemolysis on potassium assays have been demonstrated in venous versus capillary samples. 87 The setting of acceptability limits is of great significance in the interpretation of results of interference studies. Manufacturers often use AE10% as their criterion of acceptability for their assay performance claims with regard to interferences (Table 2).…”

Section: Interference Testingmentioning

confidence: 99%

Serum indices: managing assay interference

Farrell¹,

Carter²

2016

Ann Clin Biochem

113

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Clinical laboratories frequently encounter samples showing significant haemolysis, icterus or lipaemia. Technical advances, utilizing spectrophotometric measurements on automated chemistry analysers, allow rapid and accurate identification of such samples. However, accurate quantification of haemolysis, icterus and lipaemia interference is of limited value if laboratories do not set rational alert limits, based on sound interference testing experiments. Furthermore, in the context of increasing consolidation of laboratories and the formation of laboratory networks, there is an increasing requirement for harmonization of the handling of haemolysis, icterus and lipaemia-affected samples across different analytical platforms. Harmonization may be best achieved by considering both the analytical aspects of index measurement and the possible variations in the effects of haemolysis, icterus and lipaemia interferences on assays from different manufacturers. Initial verification studies, followed up with ongoing quality control testing, can help a laboratory ensure the accuracy of haemolysis, icterus and lipaemia index results, as well as assist in managing any biases in index results from analysers from different manufacturers. Similarities, and variations, in the effect of haemolysis, icterus and lipaemia interference in assays from different manufacturers can often be predicted from the mechanism of interference. Nevertheless, interference testing is required to confirm expected similarities or to quantify differences. It is important that laboratories are familiar with a number of interference testing protocols and the particular strengths and weaknesses of each. A rigorous approach to all aspects of haemolysis, icterus and lipaemia interference testing allows the analytical progress in index measurement to be translated into improved patient care.

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Potassium but Not Lactate Dehydrogenase Elevation Due To In Vitro Hemolysis Is Higher in Capillary Than in Venous Blood Samples

Cited by 11 publications

References 20 publications

Microfluidic blood plasma separation for medical diagnostics: is it worth it?

Microfluidic blood plasma separation for medical diagnostics: is it worth it?

Elevation of serum lactate dehydrogenase in patients with pectus excavatum

Serum indices: managing assay interference

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